Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,046,230 C→G 7.9% M717I (ATG→ATC)  PP_0906 ← RND family multidrug transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,046,2300CG 7.9% 153.9 / 5.5 51M717I (ATG→ATC) PP_0906RND family multidrug transporter
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base G (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

TTCACGTAGTAGTTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCCAGCAGTGCCGG  >  NC_002947/1046084‑1046379
                                                                                                                                                  |                                                                                                                                                     
ttcACGTAGTAGTTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATc                                                                                                                                                    <  2:208172/150‑1 (MQ=255)
ttcACGTAGTAGTTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATc                                                                                                                                                    <  1:634527/150‑1 (MQ=255)
     gtagtaGTTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGacac                                                                                                                                               >  1:131813/1‑150 (MQ=255)
        gtagtTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAACGGTCCTACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTCCACCCGAGGGTGTCACTCGACAcccc                                                                                                                                            >  1:46529/1‑150 (MQ=255)
                  agccagccGGGGTTGTCGCGGGAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCCAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGcgcc                                                                                                                                  <  2:338934/150‑1 (MQ=255)
                    ccagccGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGcgccgc                                                                                                                                >  2:307797/1‑150 (MQ=255)
                      agccGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCt                                                                                                                              >  1:714058/1‑150 (MQ=255)
                               tGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGt                                                                                                                     <  2:360684/150‑1 (MQ=255)
                                           gCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCAAGACGAGTTGGCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTccacca                                                                                                         >  2:436304/1‑150 (MQ=255)
                                                     cTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTccacca                                                                                                         >  1:18565/1‑140 (MQ=255)
                                                     cTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTccacca                                                                                                         <  2:18565/140‑1 (MQ=255)
                                                     cTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCggg                                                                                               <  2:67755/150‑1 (MQ=255)
                                                        ggCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGttt                                                                                            <  2:208443/150‑1 (MQ=255)
                                                               aCCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTc                                                                                                              <  2:511628/125‑1 (MQ=255)
                                                               aCCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTc                                                                                                              >  1:511628/1‑125 (MQ=255)
                                                                 cTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGt                                                                                                               >  2:266079/1‑122 (MQ=255)
                                                                 cTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGt                                                                                                               <  1:266079/122‑1 (MQ=255)
                                                                  ttGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGcgccgc                                                                                                                                <  2:407378/104‑1 (MQ=255)
                                                                  ttGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGcgccgc                                                                                                                                >  1:407378/1‑104 (MQ=255)
                                                                             gCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGGGGGTGTCGATGGACAccc                                                                                                                                             >  2:299959/1‑80 (MQ=255)
                                                                             gCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGGGGGTGTCGATGGACAccc                                                                                                                                             <  1:299959/80‑1 (MQ=255)
                                                                             gCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGaa                                                                                      >  2:238093/1‑135 (MQ=255)
                                                                             gCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGaa                                                                                      <  1:238093/135‑1 (MQ=255)
                                                                               cTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGa                                                                     >  1:570142/1‑150 (MQ=255)
                                                                                ttCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTgc                                                                                                                                     >  2:323075/1‑85 (MQ=255)
                                                                                ttCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTgc                                                                                                                                     <  1:323075/85‑1 (MQ=255)
                                                                                    aGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGCCATTCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAg                                                                <  1:236658/150‑1 (MQ=255)
                                                                                    aGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAg                                                                <  2:131813/150‑1 (MQ=255)
                                                                                      ggTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTGTCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGc                                                              <  1:569155/150‑1 (MQ=255)
                                                                                        tGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGa                                                            >  2:550287/1‑150 (MQ=255)
                                                                                          aaaCGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTgcgc                                                                                                                                   <  2:114190/77‑1 (MQ=255)
                                                                                          aaaCGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTgcgc                                                                                                                                   >  1:114190/1‑77 (MQ=255)
                                                                                               gTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGCTGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGcc                                                     >  1:453842/1‑150 (MQ=255)
                                                                                                    tttCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGaa                                                           <  2:193361/139‑1 (MQ=255)
                                                                                                    tttCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGaa                                                           >  1:193361/1‑139 (MQ=255)
                                                                                                            tCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAgcg                                        >  1:123854/1‑150 (MQ=255)
                                                                                                              gCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAgcgcg                                      >  2:94294/1‑150 (MQ=255)
                                                                                                               cccAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAgcgcgc                                     <  2:714058/150‑1 (MQ=255)
                                                                                                                   gcagGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATc                                                                             >  2:244900/1‑106 (MQ=255)
                                                                                                                   gcagGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATc                                                                             <  1:244900/106‑1 (MQ=255)
                                                                                                                       ggTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTccaccacca                                                                                                      >  1:526041/1‑77 (MQ=255)
                                                                                                                       ggTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTccaccacca                                                                                                      <  2:526041/77‑1 (MQ=255)
                                                                                                                         tCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACtt                                                        <  1:547651/121‑1 (MQ=255)
                                                                                                                         tCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACtt                                                        >  2:547651/1‑121 (MQ=255)
                                                                                                                               aGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTggg                     <  1:550287/150‑1 (MQ=255)
                                                                                                                                 gTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGcc                   <  2:570142/150‑1 (MQ=255)
                                                                                                                                      gatgggggTGTCGATGGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCtt                                <  2:332077/127‑1 (MQ=255)
                                                                                                                                      gatgggggTGTCGATGGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCtt                                >  1:332077/6‑132 (MQ=255)
                                                                                                                                       ccgAAGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGcc                                                     >  2:214738/1‑110 (MQ=255)
                                                                                                                                       ccgAAGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGcc                                                     <  1:214738/110‑1 (MQ=255)
                                                                                                                                         gAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATCCGTTGGGCCACGTCcag           <  1:436304/150‑1 (MQ=255)
                                                                                                                                                  cATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGaa                                                           <  1:736528/93‑1 (MQ=255)
                                                                                                                                                  cATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGaa                                                           >  2:736528/1‑93 (MQ=255)
                                                                                                                                                  cATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCCAGCAGTGCCgg  >  1:134962/1‑150 (MQ=255)
                                                                                                                                                  cATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCCAGCAGTGCCgg  <  1:307797/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TTCACGTAGTAGTTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCCAGCAGTGCCGG  >  NC_002947/1046084‑1046379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑