Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	86,245	A→C	20.7%	M158R (ATG→AGG)	PP_0075 ←	choline sulfate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	86,245	0	A	C	20.7%	50.5 / 13.3	29	M158R (ATG→AGG)	PP_0075	choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base A (15/8);  new base C (3/3);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

CGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGTC  >  NC_002947/86107‑86394                                                                                                                                           |                                                                                                                                                      cGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAgg                                                                                                                                            <  1:157659/150‑1 (MQ=255)        gcCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTa                                                                                                                                     >  2:331152/1‑150 (MQ=255)          cAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCca                                                                                                                                                 <  2:687378/136‑1 (MQ=255)          cAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCca                                                                                                                                                 >  1:687378/1‑136 (MQ=255)               tttGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcagc                                                                                                                                               <  1:338767/133‑1 (MQ=255)               tttGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcagc                                                                                                                                               >  2:338767/1‑133 (MQ=255)                                            ccAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCg                                                                                                 >  1:180784/1‑150 (MQ=255)                                                                 gtaggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGAAAGGACTAGCACCACGGAAt                                                                            >  2:511778/1‑150 (MQ=255)                                                                         aCCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACg                                                                    >  1:57212/1‑150 (MQ=255)                                                                           cTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGaa                                                                  >  1:176687/1‑150 (MQ=255)                                                                            tCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAg                                                                 <  2:57212/150‑1 (MQ=255)                                                                               ccTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGa                                                                              <  1:211447/134‑1 (MQ=255)                                                                               ccTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGa                                                                              >  2:211447/1‑134 (MQ=255)                                                                                cTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGa                                                             >  2:449711/1‑150 (MQ=255)                                                                                                   cTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCaa                                          >  2:170256/1‑150 (MQ=255)                                                                                                       aCGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGc                                      <  1:84250/150‑1 (MQ=255)                                                                                                       aCGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGc                                      <  1:331152/150‑1 (MQ=255)                                                                                                           tcatcaGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGa                                  >  2:217347/1‑150 (MQ=255)                                                                                                                          acctgctggGCATGGCCCTGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGcacc                                                                                   <  1:724458/82‑1 (MQ=38)                                                                                                                          acctgctggGCATGGCCCTGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGcacc                                                                                   >  2:724458/5‑86 (MQ=38)                                                                                                                            tggctggGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATg                 >  2:591899/1‑150 (MQ=255)                                                                                                                            tggctggGCAGAGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAACAGCAGCTGGagcgtg                 >  2:3747/1‑145 (MQ=255)                                                                                                                                    cAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAg         >  2:533432/1‑150 (MQ=255)                                                                                                                                     aGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGa                                                                                        >  1:732973/1‑70 (MQ=255)                                                                                                                                     aGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGa                                                                                        <  2:732973/70‑1 (MQ=255)                                                                                                                                         ccATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAg                                                                 >  1:943/1‑89 (MQ=255)                                                                                                                                         ccATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAg                                                                 <  2:943/89‑1 (MQ=255)                                                                                                                                          cATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATgg                                                                          <  2:178134/79‑1 (MQ=255)                                                                                                                                          cATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATgg                                                                          >  1:178134/1‑79 (MQ=255)                                                                                                                                           aTGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGTGAGACGAACTTCACAACCGAACCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGTc  >  1:168357/1‑150 (MQ=255)                                                                                                                                           aTGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGTc  >  2:547045/1‑150 (MQ=255)                                                                                                                                           |                                                                                                                                                      CGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGTC  >  NC_002947/86107‑86394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑