| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,178,716 | C→T | 11.8% | intergenic (+15/+79) | guaA → / ← PP_1033 | glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,178,716 | 0 | C | T | 11.8% | 105.5 / 4.0 | 42 | intergenic (+15/+79) | guaA/PP_1033 | glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (19/18); new base T (3/2); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TTGGGCACACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGC > NC_002947/1178575‑1178856 | ttGGGCACACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑c > 1:89953/1‑150 (MQ=255) gggCACACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑Ac < 1:684483/150‑1 (MQ=255) gcgCACACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCTGGGCTGG‑cta < 1:250059/148‑3 (MQ=255) gCACACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑Accc > 2:393684/1‑150 (MQ=255) tGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGGTACTATCGAGTGGGAGTCATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGAc < 2:145155/150‑1 (MQ=255) tGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGAc < 1:423179/150‑1 (MQ=255) aCGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATac < 2:89953/150‑1 (MQ=255) gctgctGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCa > 2:80630/1‑150 (MQ=255) tGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑At < 1:628347/150‑1 (MQ=255) tGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑At > 2:231872/1‑150 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTg < 1:231872/150‑1 (MQ=255) tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTgg > 1:564018/1‑150 (MQ=255) aTCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTgtt < 1:258367/150‑1 (MQ=255) aCGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTgttcgtt > 1:433762/1‑150 (MQ=255) ggTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCa < 1:80630/150‑1 (MQ=255) gTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAg > 2:346135/1‑150 (MQ=255) gcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCtt > 1:541102/1‑150 (MQ=255) gacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCtt > 2:556412/1‑150 (MQ=255) tCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCTGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGc > 2:51869/1‑150 (MQ=255) gccgccTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTgg < 2:80221/101‑1 (MQ=255) gccgccTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTgg > 1:80221/1‑101 (MQ=255) cgccTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCt > 1:83010/1‑150 (MQ=255) ccACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAGGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTgg < 2:330300/150‑1 (MQ=255) ccACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTgg < 2:530925/150‑1 (MQ=255) cACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct < 2:672968/122‑1 (MQ=255) cACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct > 1:672968/1‑122 (MQ=255) tATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGCAATCA‑TGCGGGCTTTCTGGt > 1:629606/1‑90 (MQ=37) tATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGCAATCA‑TGCGGGCTTTCTGGt < 2:629606/90‑1 (MQ=37) tGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTAttc > 1:635548/1‑108 (MQ=255) tGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTAttc < 2:635548/108‑1 (MQ=255) tGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCAGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtata < 2:433762/150‑1 (MQ=255) gAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGa < 1:556412/150‑1 (MQ=255) gTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGaaa < 2:541102/150‑1 (MQ=255) tCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAagcag > 2:341417/1‑150 (MQ=255) gggTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGa > 1:236835/1‑142 (MQ=255) gggTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGa < 2:236835/142‑1 (MQ=255) gTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTg > 2:569471/1‑150 (MQ=255) gTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTg > 2:101179/1‑150 (MQ=255) gtgccagccTGGGCTGGCC‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTg < 2:729208/73‑1 (MQ=255) gtgccagccTGGGCTGGCC‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTg > 1:729208/3‑75 (MQ=38) gtgccagccTGGGCTGG‑CTACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTCGGCCTTATAGGGGAGCAGCCTg > 2:606266/3‑150 (MQ=255) gtgccagccTGGGCTGG‑C‑GTCCGCAGGACGGATACACCAGAACGCCTGC‑GTGATTGCGGGCTTTATGGTTCTCGTTCGCTCAGATTTCTTATTCTTCTTGCTTTCTGGCAAATAGCGCTTTCTAGTAGGCGTTATAGGAAAGCAGCCTGc > 2:105998/3‑150 (MQ=255) | TTGGGCACACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGG‑C‑ACCCGCAGGACGGATACACCAGAAAGCCCGC‑ATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGC > NC_002947/1178575‑1178856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |