Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,192,686 T→C 8.1% K324E (AAA→GAA)  upxB ← phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,192,6860TC 8.1% 137.3 / 2.4 49K324E (AAA→GAA) upxBphosphatase
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base C (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

AGGCCGGGGGCGCAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGCCC  >  NC_002947/1192541‑1192831
                                                                                                                                                 |                                                                                                                                                 
aGGCCGGGGGCGCAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACg                                                                                                                                               >  2:730465/1‑150 (MQ=255)
aGGCCGGGGGCGCAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACg                                                                                                                                               <  1:437843/150‑1 (MQ=255)
           gcAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTcaaa                                                                                                                                    >  1:233898/1‑147 (MQ=255)
           gcAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTCaaa                                                                                                                                    >  1:233434/1‑150 (MQ=255)
             aGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTCAAAgg                                                                                                                                  <  2:233434/150‑2 (MQ=255)
             aGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTCAAAgg                                                                                                                                  <  2:233898/150‑2 (MQ=255)
                    gTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGaa                                                                                                                           >  2:168534/1‑150 (MQ=255)
                    gTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGaa                                                                                                                           <  1:304637/150‑1 (MQ=255)
                               ggCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCa                                                                                                                <  2:484293/150‑1 (MQ=255)
                                 cccAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACt                                                                                                              <  1:483825/150‑1 (MQ=255)
                                  ccAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTc                                                                                                                                       >  1:679371/1‑124 (MQ=255)
                                  ccAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTc                                                                                                                                       <  2:679371/124‑1 (MQ=255)
                                     gcgcGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGc                                                                                                          <  2:396372/150‑1 (MQ=255)
                                       gcgGGGGGTTTGGCGGCCAGGGTGCTGCTGTTGTACGGGTCGGTCTCGAGGATGTAGCGGTGGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGGATTGTTCGTCCGCTTCGGTg                                                                                                        <  2:224836/150‑1 (MQ=255)
                                              atctggtggCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGAAAATGCGTCGTCCACTTCGCTGGCGTCAc                                                                                                 <  1:395872/147‑1 (MQ=255)
                                               tttGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCAcc                                                                                                <  2:721650/150‑1 (MQ=255)
                                                   gtggCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGCAGCATACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCAccagat                                                                                            >  2:395872/1‑146 (MQ=255)
                                                                tgctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGAc                                                                                                                                                <  1:219062/85‑1 (MQ=255)
                                                                tgctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGAc                                                                                                                                                >  2:219062/1‑85 (MQ=255)
                                                                tgctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACt                                                                               >  2:701583/1‑150 (MQ=255)
                                                                 gctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATtcg                                                                                                                       >  2:8452/1‑109 (MQ=255)
                                                                 gctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATtcg                                                                                                                       <  1:8452/109‑1 (MQ=255)
                                                                         aCGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGGCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGc                                                                      <  1:168534/150‑1 (MQ=255)
                                                                           gggTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGACCCGTTGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCg                                                                    >  1:13273/1‑150 (MQ=255)
                                                                                gATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGa                                                               >  2:132894/1‑150 (MQ=255)
                                                                                    gcgatgaTGTAGCCGTCCGTGCTCGCTTCGTTGCGATGGTCGTCGGTGGCGCTGGCGCCGTTGACGGTAACGTCGAAGCGGGCGCATTCGTCGTCCACCTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTg                                                           <  2:467308/149‑1 (MQ=255)
                                                                                      gacgaTGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCggg                                                                                                                               >  2:588899/1‑80 (MQ=255)
                                                                                      gacgaTGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCggg                                                                                                                               <  1:588899/80‑1 (MQ=255)
                                                                                            gTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGcc                                                   >  2:466972/1‑150 (MQ=255)
                                                                                             tAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCa                                                  >  1:76853/1‑150 (MQ=255)
                                                                                                  gTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATgcg                                             >  2:236452/1‑150 (MQ=255)
                                                                                                            gCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGc                                                                                                          >  2:68814/1‑79 (MQ=255)
                                                                                                            gCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGc                                                                                                          <  1:68814/79‑1 (MQ=255)
                                                                                                            gCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTcc                                   >  2:717271/1‑150 (MQ=255)
                                                                                                              ttCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTNGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGACCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTc                                 >  1:193299/1‑150 (MQ=255)
                                                                                                                cGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCgg                               <  2:13273/150‑1 (MQ=255)
                                                                                                                 gTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTg                                                                                                        <  1:628516/76‑1 (MQ=255)
                                                                                                                 gTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTg                                                                                                        >  2:628516/1‑76 (MQ=255)
                                                                                                                 gTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCAGCTGAAAGTGCCCACGCCAATGCGGCGCTGGTCCTCggg                              >  2:364171/1‑150 (MQ=255)
                                                                                                                      gATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGTGGGCGAATTCGTCGTCCACTTCGCTTGCTTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCggg                         >  1:14577/1‑150 (MQ=255)
                                                                                                                      gATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCggg                         >  1:65791/1‑150 (MQ=255)
                                                                                                                       aTAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGt                        <  2:491319/150‑1 (MQ=255)
                                                                                                                        tAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATgcgg                                            <  1:495047/129‑1 (MQ=255)
                                                                                                                        tAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATgcgg                                            >  2:495047/1‑129 (MQ=255)
                                                                                                                             gtcgGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCttt                  <  1:132894/150‑1 (MQ=255)
                                                                                                                             gtcgGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTAGTCGTCCAAATCGCTGACGTCAACAGCAGCGCTTAGGGAAAGAGAGTTGCGGCTGGAGTTGCTCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCttt                  >  2:460460/1‑150 (MQ=255)
                                                                                                                                ggTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTttgtt               <  1:234343/150‑1 (MQ=255)
                                                                                                                                           cgcCTTTGACGGTAACGTCGAAGCTGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCTCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGc    <  1:236452/150‑1 (MQ=255)
                                                                                                                                             ccTTTGACGGTAACGTCGAAGCGGGCGCATTCGTTCTACCCCGCGCTGGCGGCACCGGCAGCGCTTTCCCACTTCTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGccc  <  1:730465/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGGCCGGGGGCGCAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGCCC  >  NC_002947/1192541‑1192831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑