Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,192,691 G→T 8.8% T322N (ACC→AAC)  upxB ← phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,192,6910GT 8.8% 134.4 / 2.4 46T322N (ACC→AAC) upxBphosphatase
Reads supporting (aligned to +/- strand):  ref base G (19/22);  new base T (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

GCAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGCCCGGCCAGTT  >  NC_002947/1192552‑1192839
                                                                                                                                           |                                                                                                                                                    
gcAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTcaaa                                                                                                                                            >  1:233898/1‑147 (MQ=255)
gcAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTCaaa                                                                                                                                            >  1:233434/1‑150 (MQ=255)
  aGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTCAAAgg                                                                                                                                          <  2:233434/150‑2 (MQ=255)
  aGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTCGACGTTACCGTCAAAgg                                                                                                                                          <  2:233898/150‑2 (MQ=255)
         gTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGaa                                                                                                                                   >  2:168534/1‑150 (MQ=255)
         gTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGaa                                                                                                                                   <  1:304637/150‑1 (MQ=255)
                    ggCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCa                                                                                                                        <  2:484293/150‑1 (MQ=255)
                      cccAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACt                                                                                                                      <  1:483825/150‑1 (MQ=255)
                       ccAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTc                                                                                                                                               <  2:679371/124‑1 (MQ=255)
                       ccAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTc                                                                                                                                               >  1:679371/1‑124 (MQ=255)
                          gcgcGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGc                                                                                                                  <  2:396372/150‑1 (MQ=255)
                            gcgGGGGGTTTGGCGGCCAGGGTGCTGCTGTTGTACGGGTCGGTCTCGAGGATGTAGCGGTGGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGGATTGTTCGTCCGCTTCGGTg                                                                                                                <  2:224836/150‑1 (MQ=255)
                                   atctggtggCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGAAAATGCGTCGTCCACTTCGCTGGCGTCAc                                                                                                         <  1:395872/147‑1 (MQ=255)
                                    tttGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCAcc                                                                                                        <  2:721650/150‑1 (MQ=255)
                                        gtggCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGCAGCATACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCAccagat                                                                                                    >  2:395872/1‑146 (MQ=255)
                                                     tgctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACt                                                                                       >  2:701583/1‑150 (MQ=255)
                                                      gctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATtcg                                                                                                                               <  1:8452/109‑1 (MQ=255)
                                                      gctgTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATtcg                                                                                                                               >  2:8452/1‑109 (MQ=255)
                                                              aCGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGGCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGc                                                                              <  1:168534/150‑1 (MQ=255)
                                                                gggTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGACCCGTTGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCg                                                                            >  1:13273/1‑150 (MQ=255)
                                                                     gATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGa                                                                       >  2:132894/1‑150 (MQ=255)
                                                                         gcgatgaTGTAGCCGTCCGTGCTCGCTTCGTTGCGATGGTCGTCGGTGGCGCTGGCGCCGTTGACGGTAACGTCGAAGCGGGCGCATTCGTCGTCCACCTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTg                                                                   <  2:467308/149‑1 (MQ=255)
                                                                           gacgaTGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCggg                                                                                                                                       >  2:588899/1‑80 (MQ=255)
                                                                           gacgaTGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCggg                                                                                                                                       <  1:588899/80‑1 (MQ=255)
                                                                                 gTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGcc                                                           >  2:466972/1‑150 (MQ=255)
                                                                                  tAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCa                                                          >  1:76853/1‑150 (MQ=255)
                                                                                       gTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATgcg                                                     >  2:236452/1‑150 (MQ=255)
                                                                                                 gCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGc                                                                                                                  <  1:68814/79‑1 (MQ=255)
                                                                                                 gCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGc                                                                                                                  >  2:68814/1‑79 (MQ=255)
                                                                                                 gCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTcc                                           >  2:717271/1‑150 (MQ=255)
                                                                                                   ttCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTNGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGACCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTc                                         >  1:193299/1‑150 (MQ=255)
                                                                                                     cGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCgg                                       <  2:13273/150‑1 (MQ=255)
                                                                                                      gTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTg                                                                                                                <  1:628516/76‑1 (MQ=255)
                                                                                                      gTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTg                                                                                                                >  2:628516/1‑76 (MQ=255)
                                                                                                      gTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCAGCTGAAAGTGCCCACGCCAATGCGGCGCTGGTCCTCggg                                      >  2:364171/1‑150 (MQ=255)
                                                                                                           gATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGTGGGCGAATTCGTCGTCCACTTCGCTTGCTTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCggg                                 >  1:14577/1‑150 (MQ=255)
                                                                                                           gATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCggg                                 >  1:65791/1‑150 (MQ=255)
                                                                                                            aTAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGt                                <  2:491319/150‑1 (MQ=255)
                                                                                                             tAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATgcgg                                                    <  1:495047/129‑1 (MQ=255)
                                                                                                             tAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATgcgg                                                    >  2:495047/1‑129 (MQ=255)
                                                                                                                  gtcgGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCttt                          <  1:132894/150‑1 (MQ=255)
                                                                                                                  gtcgGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTAGTCGTCCAAATCGCTGACGTCAACAGCAGCGCTTAGGGAAAGAGAGTTGCGGCTGGAGTTGCTCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCttt                          >  2:460460/1‑150 (MQ=255)
                                                                                                                     ggTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTttgtt                       <  1:234343/150‑1 (MQ=255)
                                                                                                                                cgcCTTTGACGGTAACGTCGAAGCTGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCTCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGc            <  1:236452/150‑1 (MQ=255)
                                                                                                                                  ccTTTGACGGTAACGTCGAAGCGGGCGCATTCGTTCTACCCCGCGCTGGCGGCACCGGCAGCGCTTTCCCACTTCTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGccc          <  1:730465/150‑1 (MQ=255)
                                                                                                                                       gACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGCCCGGCCa     <  1:701583/150‑1 (MQ=255)
                                                                                                                                          ggTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGTGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGCCCGGCCAGtt  <  1:717271/150‑1 (MQ=255)
                                                                                                                                          ggTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGCCCGGCCAGtt  <  2:76853/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
GCAGCCCTCGTGACGGAAGCGGCCCAGCGCGGTGCGTTTGGTGGCCAGGGTGCTGCTGTTGTACGGGTCGATCTCGACGATGTAGCCGTAGGTGCTGGCTTCGTTGCGATAGTCGTCGGTGGCGCTGGCGCCTTTGACGGTAACGTCGAAGCGGGCGAATTCGTCGTCCACTTCGCTGGCGTCACCGGCAGCGCTTTCCCACTTGTACTGGCCGCTGGAAGTGCCCACGCCAATGCGGCGCTGGTCCTCGGGGCGGGTGCCTTTGTTGACGAAAATGCCCGGCCAGTT  >  NC_002947/1192552‑1192839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑