Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 87,493 T→A 10.5% N98I (AAT→ATT)  PP_0076 ← choline betaine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294787,4930TA10.5% 132.6 / 5.7 46N98I (AAT→ATT) PP_0076choline betaine‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base A (2/3);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACAGG  >  NC_002947/87350‑87642
                                                                                                                                               |                                                                                                                                                     
ttCCGTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGa                                                                                                                                                 <  2:88584/150‑1 (MQ=255)
  ccGTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAATGGCGATg                                                                                                                                               <  2:533541/150‑1 (MQ=255)
     tACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAAc                                                                                                                                            <  2:581170/150‑1 (MQ=255)
                 tCGAACTGCCCGGCGTGTTTTTTTTTTTTTTTGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAg                                                                                                                                >  2:663284/1‑150 (MQ=255)
                   gAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGAt                                                                                                                                                <  2:314882/132‑1 (MQ=255)
                   gAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGAt                                                                                                                                                >  1:314882/1‑132 (MQ=255)
                   gAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGcc                                                                                                                              >  1:8363/1‑150 (MQ=255)
                      cTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTtg                                                                                                                           <  1:257943/150‑1 (MQ=255)
                       tGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAAGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTtgc                                                                                                                          <  1:236773/150‑1 (MQ=255)
                        gCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTtgct                                                                                                                         <  2:327521/150‑1 (MQ=255)
                             gCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGcc                                                                                                                    <  2:499361/150‑1 (MQ=255)
                              cGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCg                                                                                                                   >  2:472289/1‑150 (MQ=255)
                                  tttttGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAtt                                                                                                                                                      >  1:742956/1‑111 (MQ=255)
                                  tttttGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAtt                                                                                                                                                      <  2:742956/111‑1 (MQ=255)
                                  tttttGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAATGGCGATgtgca                                                                                                                                           <  1:432102/122‑5 (MQ=255)
                                  tttttGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCAATGGCGATgtgca                                                                                                                                           >  2:432102/1‑118 (MQ=255)
                                          ggTAAGCGAAGTCCTTCACCCCGGCGTTCCACACATAGTCGGGAACTGCGAGGGTGAACTTGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGAAGAACTTGTCATTGAAGCCTTTCTGCGCCGGCATCCAGtttt                                                                                                       >  2:133262/1‑149 (MQ=255)
                                           gTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTcc                                                                                                      <  2:337429/150‑1 (MQ=255)
                                             cAGCGAAGTCCTTCACCCCGGCATTCCAAACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGa                                                                                                    <  2:543725/150‑1 (MQ=255)
                                              aGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAg                                                                                                   >  2:580240/1‑150 (MQ=255)
                                                       cTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTc                                                                                          >  2:440848/1‑150 (MQ=255)
                                                       cTTCACCCCGGCACTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTc                                                                                          >  2:429812/1‑150 (MQ=255)
                                                        ttCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCg                                                                                         >  1:17459/1‑150 (MQ=255)
                                                          cACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGAc                                                                                       <  2:8363/150‑1 (MQ=255)
                                                              ccGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTgg                                                                                   >  2:317643/1‑150 (MQ=255)
                                                               cGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCCTGGCAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGc                                                                                  >  1:352108/1‑150 (MQ=255)
                                                                      ccACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCtt                                                                           <  1:472289/150‑1 (MQ=255)
                                                                        acacaTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGCAAGGCGTCGACCTGGCCGTCCTTGa                                                                         >  2:360616/1‑150 (MQ=255)
                                                                        acacaTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAACCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGa                                                                         <  1:133262/150‑1 (MQ=255)
                                                                         cacaTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAg                                                                                                   <  1:368928/123‑1 (MQ=255)
                                                                         cacaTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAg                                                                                                   >  2:368928/1‑123 (MQ=255)
                                                                         cacaTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAg                                                                        <  2:688864/150‑1 (MQ=255)
                                                                           caTAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGgc                                                                      >  1:664223/1‑150 (MQ=255)
                                                                                    gCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTAATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAga                                                             >  2:550482/1‑150 (MQ=255)
                                                                                                       ggTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCt                                          >  1:510376/1‑150 (MQ=255)
                                                                                                              tCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTc                                       <  2:18121/146‑1 (MQ=255)
                                                                                                              tCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTc                                       >  1:18121/1‑146 (MQ=255)
                                                                                                                      gcgcGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTg                           <  2:303708/150‑1 (MQ=255)
                                                                                                                      gcgcGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTAATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTg                           <  1:550482/150‑1 (MQ=255)
                                                                                                                              gctgctGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCa                   <  2:510376/150‑1 (MQ=255)
                                                                                                                                    gttcaTCGCCAATGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAg                  <  2:567035/142‑1 (MQ=255)
                                                                                                                                    gttcaTCGCCAATGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAg                  >  1:567035/4‑145 (MQ=255)
                                                                                                                                    gcACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTg                           >  2:669832/1‑136 (MQ=255)
                                                                                                                                    gcACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTg                           <  1:669832/136‑1 (MQ=255)
                                                                                                                                    gcACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCAGTATATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCttt             >  1:727432/1‑150 (MQ=255)
                                                                                                                                           gCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTaa      <  2:17459/150‑1 (MQ=255)
                                                                                                                                             cATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACa    >  1:181202/1‑150 (MQ=255)
                                                                                                                                             cATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACa    >  1:544447/1‑150 (MQ=255)
                                                                                                                                               ttGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACAgg  <  2:664223/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
TTCCGTACAGTTTTTTGTCGAACTGCCCGGCGTGTTTTTGCAGGTCAGCGAAGTCCTTCACCCCGGCATTCCACACATAGTCGGGCACTGCGAGGGTGAACTCGGTTCCCTCCAGGTTGCGCGACAGCTGCTGCACATCGCCATTGGCGATGAACTTGTCATGGAAGCCTTGCTGCGCCGGCATCCAGTTTCCGAGGAAGGCGTCGACCTGGCCGTCCTTGAGGCCGCCGTAGATGATCGGTACGGCCAGGCTGTCGATCTTCACCTGGTAACCCAGGCTTTCCAGTAACAGG  >  NC_002947/87350‑87642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑