Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,207,680 G→A 9.3% L443L (CTG→TTG)  PP_1058 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,207,6800GA 9.3% 113.3 / 3.3 44L443L (CTG→TTG) PP_1058hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base A (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

CCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTGCGC  >  NC_002947/1207531‑1207824
                                                                                                                                                     |                                                                                                                                                
ccGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAg                                                                                                                                                  >  1:137489/1‑150 (MQ=255)
ccGCGAGCCATCGGCGGTGCGTTCGAACAAGGGGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCCATCCATGACTTGGATCAGCTCAACCGCTTACTGCGGGTCGGCAAGGTTTTTAAGGTACTGGCTAGCCTGCAg                                                                                                                                                  >  1:464255/1‑150 (MQ=255)
      gCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCACTTGCat                                                                                                                                            <  2:592621/150‑2 (MQ=255)
        cATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGt                                                                                                                                          >  2:402136/1‑150 (MQ=255)
                         aaCAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGATACTGTGTAGCTTTCAGTTGCAGGTCGGCCTTCAAGGTACtg                                                                                                                         >  2:59892/1‑150 (MQ=255)
                          aCAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtgg                                                                                                                        <  1:211372/150‑1 (MQ=255)
                              ggTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGc                                                                                                                    >  1:746714/1‑150 (MQ=255)
                              ggTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGc                                                                                                                    >  1:667048/1‑150 (MQ=255)
                                 gAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGa                                                                                                                 >  1:426228/1‑150 (MQ=255)
                                    gCTGTAGCCCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAg                                                                                                              >  1:316412/1‑150 (MQ=255)
                                      tGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAACTGCAGGtta                                                                                                                                        >  1:2222/1‑120 (MQ=255)
                                      tGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAACTGCAGGtta                                                                                                                                        <  2:2222/122‑3 (MQ=255)
                                               ccTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGt                                                                                                   <  2:426228/150‑1 (MQ=255)
                                                cTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCGGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTc                                                                                                  >  2:79791/1‑150 (MQ=255)
                                                                gCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCggg                                                                                  <  1:744726/150‑1 (MQ=255)
                                                                    tGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAt                                                                              >  1:640969/1‑150 (MQ=255)
                                                                       gcagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGt                                                                                             >  2:715468/1‑132 (MQ=255)
                                                                       gcagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGt                                                                                             <  1:715468/132‑1 (MQ=255)
                                                                        cagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAg                                                                                               <  1:444888/129‑1 (MQ=255)
                                                                        cagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAg                                                                                               >  2:444888/1‑129 (MQ=255)
                                                                          gcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCa                                                                        >  2:241070/1‑150 (MQ=255)
                                                                             gCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtgg                                                                                                                     >  1:499027/1‑102 (MQ=255)
                                                                             gCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtgg                                                                                                                     <  2:499027/102‑1 (MQ=255)
                                                                                    gATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGCCAg                                                              <  1:402136/150‑1 (MQ=255)
                                                                                    gATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAg                                                              <  2:549302/150‑1 (MQ=255)
                                                                                      tCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCGGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGc                                                            <  1:79791/150‑1 (MQ=255)
                                                                                         ggCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGt                                                         <  2:523421/150‑1 (MQ=255)
                                                                                                         aaaCTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGAt                                         <  1:241070/150‑1 (MQ=255)
                                                                                                             tCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGGGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGccc                                     <  2:137489/150‑1 (MQ=255)
                                                                                                                      gCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTTCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACTATCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTa                            <  1:59892/150‑1 (MQ=255)
                                                                                                                            tttttCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATc                      >  2:699278/1‑150 (MQ=255)
                                                                                                                             ttttCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCg                     <  1:699278/150‑1 (MQ=255)
                                                                                                                                   ggTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAtt                                                                             >  1:573952/1‑88 (MQ=255)
                                                                                                                                   ggTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAtt                                                                             <  2:573952/88‑1 (MQ=255)
                                                                                                                                       cTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGt           <  2:640969/150‑1 (MQ=255)
                                                                                                                                          gCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGa                                                                           >  1:674758/1‑83 (MQ=255)
                                                                                                                                          gCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGa                                                                           <  2:674758/83‑1 (MQ=255)
                                                                                                                                           cTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGc       <  2:464255/150‑1 (MQ=255)
                                                                                                                                            cgaCCTGCAACTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgttg                               <  2:116360/123‑1 (MQ=255)
                                                                                                                                            cgaCCTGCAACTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgttg                               >  1:116360/3‑125 (MQ=255)
                                                                                                                                             aaCCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTg     >  2:124790/1‑150 (MQ=255)
                                                                                                                                               ccTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGCGTGTTTTGTACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTgcg   >  1:610413/1‑150 (MQ=255)
                                                                                                                                               ccTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTgcg   <  2:667048/150‑1 (MQ=255)
                                                                                                                                                cTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgt                                 >  1:222812/1‑119 (MQ=255)
                                                                                                                                                cTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgt                                 <  2:222812/119‑1 (MQ=255)
                                                                                                                                                cTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTgcgc  >  2:316717/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCGCGAGCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTGCGC  >  NC_002947/1207531‑1207824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑