Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,207,681 T→C 9.1% Q442Q (CAA→CAG)  PP_1058 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,207,6810TC 9.1% 119.0 / 3.3 44Q442Q (CAA→CAG) PP_1058hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/21);  new base C (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

GCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTGCGC  >  NC_002947/1207537‑1207824
                                                                                                                                                |                                                                                                                                               
gCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCACTTGCat                                                                                                                                            <  2:592621/150‑2 (MQ=255)
  cATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGt                                                                                                                                          >  2:402136/1‑150 (MQ=255)
                   aaCAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGATACTGTGTAGCTTTCAGTTGCAGGTCGGCCTTCAAGGTACtg                                                                                                                         >  2:59892/1‑150 (MQ=255)
                    aCAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtgg                                                                                                                        <  1:211372/150‑1 (MQ=255)
                        ggTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGc                                                                                                                    >  1:746714/1‑150 (MQ=255)
                        ggTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGc                                                                                                                    >  1:667048/1‑150 (MQ=255)
                           gAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGa                                                                                                                 >  1:426228/1‑150 (MQ=255)
                              gCTGTAGCCCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAg                                                                                                              >  1:316412/1‑150 (MQ=255)
                                tGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAACTGCAGGtta                                                                                                                                        >  1:2222/1‑120 (MQ=255)
                                tGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAACTGCAGGtta                                                                                                                                        <  2:2222/122‑3 (MQ=255)
                                         ccTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGt                                                                                                   <  2:426228/150‑1 (MQ=255)
                                          cTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCGGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTc                                                                                                  >  2:79791/1‑150 (MQ=255)
                                                          gCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCggg                                                                                  <  1:744726/150‑1 (MQ=255)
                                                              tGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAt                                                                              >  1:640969/1‑150 (MQ=255)
                                                                 gcagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGt                                                                                             >  2:715468/1‑132 (MQ=255)
                                                                 gcagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGt                                                                                             <  1:715468/132‑1 (MQ=255)
                                                                  cagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAg                                                                                               <  1:444888/129‑1 (MQ=255)
                                                                  cagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAg                                                                                               >  2:444888/1‑129 (MQ=255)
                                                                    gcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCa                                                                        >  2:241070/1‑150 (MQ=255)
                                                                       gCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtgg                                                                                                                     >  1:499027/1‑102 (MQ=255)
                                                                       gCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtggtgg                                                                                                                     <  2:499027/102‑1 (MQ=255)
                                                                              gATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGCCAg                                                              <  1:402136/150‑1 (MQ=255)
                                                                              gATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAg                                                              <  2:549302/150‑1 (MQ=255)
                                                                                tCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCGGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGc                                                            <  1:79791/150‑1 (MQ=255)
                                                                                   ggCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGt                                                         <  2:523421/150‑1 (MQ=255)
                                                                                                   aaaCTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGAt                                         <  1:241070/150‑1 (MQ=255)
                                                                                                       tCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGGGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGccc                                     <  2:137489/150‑1 (MQ=255)
                                                                                                                gCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTTCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACTATCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTa                            <  1:59892/150‑1 (MQ=255)
                                                                                                                      tttttCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATc                      >  2:699278/1‑150 (MQ=255)
                                                                                                                       ttttCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCg                     <  1:699278/150‑1 (MQ=255)
                                                                                                                             ggTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAtt                                                                             >  1:573952/1‑88 (MQ=255)
                                                                                                                             ggTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAtt                                                                             <  2:573952/88‑1 (MQ=255)
                                                                                                                                 cTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGt           <  2:640969/150‑1 (MQ=255)
                                                                                                                                    gCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGa                                                                           >  1:674758/1‑83 (MQ=255)
                                                                                                                                    gCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGa                                                                           <  2:674758/83‑1 (MQ=255)
                                                                                                                                     cTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGc       <  2:464255/150‑1 (MQ=255)
                                                                                                                                      cgaCCTGCAACTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgttg                               <  2:116360/123‑1 (MQ=255)
                                                                                                                                      cgaCCTGCAACTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgttg                               >  1:116360/3‑125 (MQ=255)
                                                                                                                                       aaCCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTg     >  2:124790/1‑150 (MQ=255)
                                                                                                                                         ccTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGCGTGTTTTGTACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTgcg   >  1:610413/1‑150 (MQ=255)
                                                                                                                                         ccTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTgcg   <  2:667048/150‑1 (MQ=255)
                                                                                                                                          cTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgt                                 >  1:222812/1‑119 (MQ=255)
                                                                                                                                          cTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgt                                 <  2:222812/119‑1 (MQ=255)
                                                                                                                                          cTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTgcgc  >  2:316717/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTGGTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTGCGC  >  NC_002947/1207537‑1207824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑