| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,265,530 | C→A | 12.9% | intergenic (‑105/+22) | PP_1106 ← / ← quiP | hypothetical protein/acyl‑homoserine lactone acylase QuiP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,265,530 | 0 | C | A | 12.9% | 80.4 / 3.3 | 31 | intergenic (‑105/+22) | PP_1106/quiP | hypothetical protein/acyl‑homoserine lactone acylase QuiP |
| Reads supporting (aligned to +/- strand): ref base C (13/14); new base A (2/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
AGTACAGGCCTTCGGGGCGGGCGATGACGAGGTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAGCGCCCTTTGAACCAGGCATCCAACCCAT > NC_002947/1265389‑1265661 | aGTACAGGCCTTCGGGGCGGGCGATGACGAGGTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGccc < 1:67881/150‑1 (MQ=255) tACAGGCCTTCGGGGCGGGCGATGACGAGGTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTAATGCGCCCCt > 1:551642/1‑150 (MQ=255) tACAGGCCTTCGGGGCGGGCGATGACGAGGTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTAATGCGCCCCt > 1:551593/1‑150 (MQ=255) gCCTTCGGGGCGGGCGATGACGAGGTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTgaaga > 2:610812/1‑150 (MQ=255) cTTCGGGGCGGGCGATGACGAGGTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAAt < 1:310695/150‑1 (MQ=255) gTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCaa < 2:369745/150‑1 (MQ=255) cATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGt > 1:9210/1‑150 (MQ=255) aTGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTa > 2:418139/1‑150 (MQ=255) tGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTAATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACg < 2:551642/150‑1 (MQ=255) tGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTAATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACg < 2:551593/150‑1 (MQ=255) ctgTTAAGAGTCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGa < 2:358174/148‑1 (MQ=255) ccGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGa > 2:36003/1‑150 (MQ=255) ccGTTAAGAGGCATGGTGCTGGACGATATATCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGa > 1:137076/1‑150 (MQ=255) ttAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGt > 2:101227/1‑150 (MQ=255) aaGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGTGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGTGCCCCTGAAGAATGCCGTTACCGcccc < 1:137257/105‑1 (MQ=255) aaGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGcccc > 2:137257/1‑105 (MQ=255) aaGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTc < 2:137076/150‑1 (MQ=255) aTGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCg < 2:244495/88‑1 (MQ=255) aTGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCg > 1:244495/1‑88 (MQ=255) gCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGt < 2:330395/150‑1 (MQ=255) aCGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAg < 1:371766/150‑1 (MQ=255) cGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAgg < 1:101227/150‑1 (MQ=255) ttCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATg > 2:266370/1‑150 (MQ=255) ttCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATg < 1:610812/150‑1 (MQ=255) ggatggatTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAg > 2:113678/1‑150 (MQ=255) ggatTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAGCGcc < 2:9210/150‑1 (MQ=255) gAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAGCGCCCTTTGAACCAGGCATCCaa > 2:448410/1‑149 (MQ=255) gAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAGCGCCCTTTGAACCAGGCATCCaa < 1:448410/149‑1 (MQ=255) aaaaaCTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTATTGCAGTGGCAGGCTCATGAAGCGCCCTTTGAACCAGGCATCCAAcc > 2:442764/1‑150 (MQ=255) aaCTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAGCGCCCTTTGAACCAGGCATCCAACCCAt < 1:418139/150‑1 (MQ=255) aaCTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAGCGCCCTTTGAACCAGGCATCCAACCCAt > 1:17974/1‑150 (MQ=255) | AGTACAGGCCTTCGGGGCGGGCGATGACGAGGTCCATGGCACGGGCACTGGTTCTGGGGTTACCCGTTAAGAGGCATGGTGCTGGACGTTAGTTCGGATGGATTTGGGAGGGAGCTGCTGAAAAACTTCATCAAGCCCCCTCATGCGCCCCTGAAGAATGCCGTTACCGCCCCGGCACCAATGTAAGCCGCTGGTTGCCATACGCCCTGCCGAAGTTCTGCTGTTGCAGTGGCAGGCTCATGAAGCGCCCTTTGAACCAGGCATCCAACCCAT > NC_002947/1265389‑1265661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |