Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,281,134 T→C 18.9% V176A (GTC→GCC)  PP_5742 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,281,1340TC18.9% 63.3 / 7.6 31V176A (GTC→GCC) PP_5742membrane protein
Reads supporting (aligned to +/- strand):  ref base T (11/14);  new base C (3/3);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACAGCTACAGGTTTGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCC  >  NC_002947/1281002‑1281280
                                                                                                                                    |                                                                                                                                                  
acacaGCTACAGGTTTGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTg                                                                                                                                                <  2:102576/137‑1 (MQ=255)
acacaGCTACAGGTTTGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTg                                                                                                                                                >  1:102576/1‑137 (MQ=255)
         cAGGTTTGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTACTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTAt                                                                                                                          <  2:512464/150‑1 (MQ=255)
          aGGTTTGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATg                                                                                                                         >  2:718246/1‑150 (MQ=255)
           ggTTTGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATgg                                                                                                                        <  2:350643/150‑1 (MQ=255)
               tGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAg                                                                                                                    <  1:718246/150‑1 (MQ=255)
                 tCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGCCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCt                                                                                                                  >  1:245118/1‑150 (MQ=255)
                 tCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGCCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCt                                                                                                                  >  1:245188/1‑150 (MQ=255)
                  cGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGCCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTa                                                                                                                 <  2:245188/150‑1 (MQ=255)
                  cGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGCCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTa                                                                                                                 <  2:245118/150‑1 (MQ=255)
                   gagaATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAg                                                                                                                >  1:60497/1‑150 (MQ=255)
                   gagaATAGTATGCCAAAGAGCCGTAGATGTATTAATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGCAGCCGCTTCTATGGGAAGCTAg                                                                                                                >  1:332492/1‑150 (MQ=255)
                                                tATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGCCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAAt                                                                                                    <  1:305117/133‑1 (MQ=255)
                                                tATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGCCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAAt                                                                                                    >  2:305117/1‑133 (MQ=255)
                                                    gATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGc                                                                               >  1:648866/1‑150 (MQ=255)
                                                      tGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCgg                                                                             <  2:386436/150‑1 (MQ=255)
                                                      tGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGAAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCgg                                                                             >  1:253884/1‑150 (MQ=255)
                                                           tATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCtt                                                                                                                              <  2:284235/96‑1 (MQ=255)
                                                           tATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCtt                                                                                                                              >  1:284235/1‑96 (MQ=255)
                                                             tGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGGAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCtatt                                                                      >  1:42890/1‑150 (MQ=255)
                                                                   gATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGt                                                                           >  1:602044/1‑139 (MQ=255)
                                                                   gATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGt                                                                           <  2:602044/139‑1 (MQ=255)
                                                                          gATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCAACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTa                                                         <  2:60497/150‑1 (MQ=255)
                                                                                tCTGTTGTTGAAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGat                                                   <  2:253884/150‑1 (MQ=255)
                                                                                                      tgtTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAgg                                                                                <  1:646621/99‑1 (MQ=255)
                                                                                                      tgtTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAgg                                                                                >  2:646621/1‑99 (MQ=255)
                                                                                                        tttttGACAGAGATGGGGCGGGAGTATGTCTTTCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAATGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGc                           <  2:332492/150‑1 (MQ=255)
                                                                                                             gACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTCTGCGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCa                      <  1:539231/150‑1 (MQ=255)
                                                                                                                agagATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGc                                                                               >  2:349424/1‑90 (MQ=255)
                                                                                                                agagATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGc                                                                               <  1:349424/90‑1 (MQ=255)
                                                                                                                                 aTGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACAGAATTAAGAGAGCCCGCCATAAGGCGGTTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTcc  <  1:645947/150‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                                  
ACACAGCTACAGGTTTGTCGAGAATAGTATGCCAAAGAGCCGTAGATGTATTGATGGGGTATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCC  >  NC_002947/1281002‑1281280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑