| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,295,024 | T→A | 20.7% | intergenic (+34/+36) | slyB → / ← nhaA‑I | outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,295,024 | 0 | T | A | 20.7% | 56.0 / 13.5 | 29 | intergenic (+34/+36) | slyB/nhaA‑I | outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA |
| Reads supporting (aligned to +/- strand): ref base T (9/14); new base A (3/3); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01 | |||||||||||
TGATGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCAC > NC_002947/1294879‑1295169 | tgattGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAATCGCGTTTCGGACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGctgctg < 1:694918/150‑1 (MQ=255) gatgGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCtgctgc > 2:553085/1‑150 (MQ=255) caTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCggg < 1:252038/150‑1 (MQ=255) caTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTAGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGAC‑TGGCAGCAGCCATGTCggg < 1:344022/150‑1 (MQ=255) cgcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGttt < 1:3027/150‑1 (MQ=255) gcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGAC‑TGGCAGCAGCCATGTCggg < 1:353855/145‑1 (MQ=255) gcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGAC‑TGGCAGCAGCCATGTCggg > 2:353855/1‑145 (MQ=255) cgcCTATGTGCAGGCCGTGCCGGCGACTGAAATTTTACGTGTTTGGGACCGTGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGttttt < 2:7376/150‑1 (MQ=255) gagaACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGAC‑TGGCAGCAGCCATGTCGGGGtt < 1:357770/125‑1 (MQ=38) gagaACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGAC‑TGGCAGCAGCCATGTCGGGGtt > 2:357770/1‑125 (MQ=38) aaCGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAAt > 1:448639/1‑150 (MQ=255) ttCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGtttt > 1:268041/1‑150 (MQ=255) ccGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGc > 1:158966/1‑150 (MQ=255) aCCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCAtt > 2:577853/1‑150 (MQ=255) gAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCg < 1:18287/150‑1 (MQ=255) cgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑c < 2:562796/150‑1 (MQ=255) tcatGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGcc > 1:703203/1‑104 (MQ=255) tcatGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGcc < 2:703203/104‑1 (MQ=255) gACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCGGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGc < 1:577853/150‑1 (MQ=255) aCCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAACCCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTGTTGCGGCTGGCCATTGCGGTGATCGGCTACGCCG‑TCACCgcaat < 1:460979/150‑3 (MQ=255) gTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAgaacga < 2:158966/150‑1 (MQ=255) cGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACAGGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCa < 1:299968/150‑1 (MQ=255) aCTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTa > 1:416996/1‑150 (MQ=255) tGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCAt < 1:553085/150‑1 (MQ=255) tGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCAt > 1:272434/1‑150 (MQ=255) aataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTc < 2:448639/150‑1 (MQ=255) aCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCgg > 2:137798/1‑150 (MQ=255) cccGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCg > 2:410040/1‑150 (MQ=255) tGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCAc < 2:268041/150‑1 (MQ=255) ggCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGACAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGc > 1:431045/1‑81 (MQ=255) ggCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGACAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGc < 2:431045/81‑1 (MQ=255) | TGATGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGA‑CGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCAC > NC_002947/1294879‑1295169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |