| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 92,066 | G→T | 10.0% | P105H (CCT→CAT) | trpA ← | tryptophan synthase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 92,066 | 0 | G | T | 10.0% | 113.3 / 2.9 | 40 | P105H (CCT→CAT) | trpA | tryptophan synthase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (20/16); new base T (2/2); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
GTCAGGCGGATGAAGTCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCAT > NC_002947/91919‑92208 | gtCAGGCGGATGAAGTCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAggg > 1:534087/1‑150 (MQ=255) aGGCGGATGAAGTCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTg < 2:498082/150‑1 (MQ=255) gCGGATGAAGTCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGCTCAGGCCCTAGACGCCCTCTTCCTTTTCTTGAGGCACTAACGTGTCCACGCCAAAGCGGTGGATAGGGTTgta < 1:14265/150‑1 (MQ=255) gCGGATGAAGTCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTgta < 2:199664/150‑1 (MQ=255) gATGAAGTCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTgtagta < 2:534087/150‑1 (MQ=255) tCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCCGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTAACGAACGTGTCAACCCCAAAGCGGTGGCTAGGGTTGTAGTAGCCCATc > 1:176291/1‑150 (MQ=255) cTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGa > 2:548277/1‑150 (MQ=255) gtggCCTGGGCCGCGGTCCCCACACCACCGTCGTGCGATGGCAACAGGTCGACGATGATCACCCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGACCACGCCAAAGCGGTGGATAGGGTTCTAGTAGCCCATCAGCACGATc < 2:39198/148‑1 (MQ=255) ggCCTGGGCAGGCGTCGCCACTTCGGCGTCGACATTGCGCGCGAGGACCGCCAGGTTCAGGCCATTGACGCCCGCTTCCTTGGCTGGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCgg > 2:7912/1‑150 (MQ=255) tGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTg > 2:494647/1‑150 (MQ=255) tGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTg > 2:494580/1‑150 (MQ=255) ggggTCGCCAGTTCGGCTTCGGGCTCGGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTgg < 2:233161/150‑1 (MQ=255) ggTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATATGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGtt > 1:110492/1‑150 (MQ=255) aGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATc < 2:241225/150‑1 (MQ=255) gCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATATGGTTGTAGTAGCCCATCa < 2:243310/124‑1 (MQ=255) gCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATATGGTTGTAGTAGCCCATCa > 1:243310/1‑124 (MQ=255) ccAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCca < 1:276158/150‑1 (MQ=255) aGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCcaca > 1:636329/1‑150 (MQ=255) gCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAAt > 1:309664/1‑150 (MQ=255) gtGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTcgcg > 2:597711/1‑150 (MQ=255) tctGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCg > 1:303812/1‑115 (MQ=255) tctGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCg < 2:303812/115‑1 (MQ=255) tGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCAt > 1:720802/1‑150 (MQ=255) gCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCt < 1:51231/150‑1 (MQ=255) ggAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGTTCGCGCGGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGc > 1:610435/1‑150 (MQ=255) gacgaTGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTGATCCACACGGAATTCGCGAACCATCTGCAGGGTCtt > 2:550949/1‑150 (MQ=255) gatgatCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTgtagta < 2:245778/81‑1 (MQ=255) gatgatCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTgtagta > 1:245778/1‑81 (MQ=255) atCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAg < 2:636329/150‑1 (MQ=255) cATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGc > 2:619853/1‑150 (MQ=255) gACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAggg > 2:155242/1‑58 (MQ=255) gACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAggg < 1:155242/58‑1 (MQ=255) ccGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCaa > 2:50176/1‑150 (MQ=255) ttCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGTGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAg > 1:524122/1‑117 (MQ=255) ttCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGTGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAg < 2:524122/117‑1 (MQ=255) ccTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGgcgc > 1:720955/1‑150 (MQ=255) ttGAGTCACGAACGTGTCTACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGCCCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGt > 2:276776/1‑150 (MQ=255) cACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCAc < 2:176291/150‑1 (MQ=255) cACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCAc > 2:143960/1‑150 (MQ=255) cAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGc < 1:167365/109‑1 (MQ=255) cAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGc > 2:167365/1‑109 (MQ=255) aaaGCGGTGGATATGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCTACgccatc < 2:110492/150‑1 (MQ=255) gTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccat < 1:597711/150‑1 (MQ=255) | GTCAGGCGGATGAAGTCGATGCCTGCGGCCTGGGCCGGGGTAGCCAGTTCGGCGTCGTGCTCTGGCGGAAGGTCGACGATGATCAGGCCATCGACGCCCGCTTCCTTGGCTTGAGTCACGAACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCAT > NC_002947/91919‑92208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |