Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,330,507 G→A 8.9% intergenic (+32/‑275) PP_5SE → / → PP_1157 5S ribosomal RNA/acetolactate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,330,5070GA 8.9% 120.7 / 3.1 45intergenic (+32/‑275)PP_5SE/PP_11575S ribosomal RNA/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base G (17/24);  new base A (2/2);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTT  >  NC_002947/1330367‑1330656
                                                                                                                                            |                                                                                                                                                     
cagagagCATTAGAACCACCTGAGCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGttt                                                                                                                                              <  2:233444/147‑1 (MQ=0)
                         ccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGAc                                                                                                                     >  2:528851/1‑150 (MQ=34)
                                 aaCTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCga                                                                                                             >  1:365291/1‑150 (MQ=39)
                                      agtagtGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCg                                                                                                        <  2:30689/150‑1 (MQ=255)
                                           tGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGAt                                                                                                   <  2:365291/150‑1 (MQ=255)
                                           tGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGAt                                                                                                   <  2:334309/150‑1 (MQ=255)
                                             aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATgg                                                                                                 >  1:65068/1‑150 (MQ=255)
                                               aCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCa                                                                                               >  2:150495/1‑150 (MQ=255)
                                                    gCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGc                                                                                                                 >  2:619618/1‑127 (MQ=37)
                                                    gCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGc                                                                                                                 <  1:619618/127‑1 (MQ=37)
                                                       tCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCa                                                                                               <  2:648225/142‑1 (MQ=255)
                                                       tCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCa                                                                                               >  1:648225/1‑142 (MQ=255)
                                                         gCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGc                                                                                     <  1:526163/150‑1 (MQ=255)
                                                         gCCGATGGTAGTGTAGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGc                                                                                     <  1:528851/150‑1 (MQ=255)
                                                                     gtgGGGTTTCCCCATGCGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCgg                                                                         <  1:448315/150‑1 (MQ=255)
                                                                     gtgCGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGGCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCgg                                                                         <  1:348915/150‑1 (MQ=255)
                                                                        gggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTa                                                                      >  2:513666/1‑150 (MQ=255)
                                                                             tCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAAccc                                                                 <  1:752149/150‑1 (MQ=255)
                                                                                 cATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTc                                                             <  1:513666/150‑1 (MQ=255)
                                                                                    gtgAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTa                                                          <  2:406698/150‑1 (MQ=255)
                                                                                     tgAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTAc                                                         >  1:406111/1‑150 (MQ=255)
                                                                                        gagTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGAAGGCCTTGTGTCTTTGTGGGCGAATAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGGAAACCCGCTCCTACaga                                                      >  1:91595/1‑150 (MQ=25)
                                                                                             ggTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATg                                                 <  1:607505/150‑1 (MQ=255)
                                                                                              gTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATgg                                                >  2:328078/1‑150 (MQ=255)
                                                                                               tCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGt                                               >  2:660007/1‑150 (MQ=255)
                                                                                                    gTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTg                                                                                                                        <  1:296102/72‑1 (MQ=34)
                                                                                                    gTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTg                                                                                                                        >  2:296102/1‑72 (MQ=34)
                                                                                                    gTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGc                                                                                          >  1:540805/1‑102 (MQ=255)
                                                                                                    gTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGc                                                                                          <  2:540805/102‑1 (MQ=255)
                                                                                                          aTTCATTTCGCAAATCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAgg                                    <  2:91595/150‑1 (MQ=255)
                                                                                                             cATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTa                                 <  2:406111/150‑1 (MQ=255)
                                                                                                             acaaagacAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTa                                 >  2:49277/8‑150 (MQ=37)
                                                                                                                  cGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATg                            <  1:150495/150‑1 (MQ=255)
                                                                                                                   acAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAgg                                    >  1:560984/2‑141 (MQ=38)
                                                                                                                   acAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAgg                                    <  2:560984/140‑1 (MQ=38)
                                                                                                                    cAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGc                          <  2:640273/150‑1 (MQ=255)
                                                                                                                     aaaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGcc                         >  2:533946/1‑150 (MQ=255)
                                                                                                                      aaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCt                        >  2:586346/1‑150 (MQ=255)
                                                                                                                       aaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTc                       <  2:733636/150‑1 (MQ=255)
                                                                                                                        aCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCa                      <  1:533946/150‑1 (MQ=255)
                                                                                                                          cccTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACACGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAAc                    >  1:161758/1‑150 (MQ=255)
                                                                                                                            cTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGt                                               >  2:122280/1‑121 (MQ=255)
                                                                                                                            cTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGt                                               <  1:122280/121‑1 (MQ=255)
                                                                                                                                     cacgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAAc         <  1:660007/150‑1 (MQ=255)
                                                                                                                                       cgcaGATAGGGGTTTTGCCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGt       <  1:49277/150‑1 (MQ=255)
                                                                                                                                            gTAGGGGTTTTGTCTTTGTGGGCAAAANATTGGACCTGCTNGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACtt  >  1:574851/1‑150 (MQ=255)
                                                                                                                                            gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCTGAGATGGCAcc                                                                                             >  2:444081/1‑59 (MQ=255)
                                                                                                                                            gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCTGAGATGGCAcc                                                                                             <  1:444081/59‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
CATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTT  >  NC_002947/1330367‑1330656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑