Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,382,332 A→T 14.9% intergenic (+12/+15) dinB → / ← PP_1204 DNA polymerase IV/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,382,3320AT14.9% 124.5 / 15.0 47intergenic (+12/+15)dinB/PP_1204DNA polymerase IV/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (19/21);  new base T (4/3);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

TGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTC  >  NC_002947/1382187‑1382472
                                                                                                                                                 |                                                                                                                                            
tGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCa                                                                                                                                          <  2:456241/150‑1 (MQ=255)
tGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCAGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCa                                                                                                                                          <  2:170399/150‑1 (MQ=255)
  gagaGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGc                                                                                                                                        >  2:454305/1‑150 (MQ=255)
       ttATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCtt                                                                                                                                   >  2:213317/1‑150 (MQ=255)
        tATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTc                                                                                                                                  <  2:253985/150‑1 (MQ=255)
          tCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAAt                                                                                                                                             <  2:397427/137‑1 (MQ=255)
          tCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAAt                                                                                                                                             >  1:397427/1‑137 (MQ=255)
          tCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTa                                                                                                                                <  1:213317/150‑1 (MQ=255)
          tCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTa                                                                                                                                >  2:424173/1‑150 (MQ=255)
          tCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTGCTa                                                                                                                                <  2:233348/150‑1 (MQ=255)
             gCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCtttcattt                                                                                                                             <  1:454305/150‑7 (MQ=255)
                     tGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGc                                                                                                                     <  1:424173/150‑1 (MQ=255)
                                    cccGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGcccc                                                                                                                                     <  1:405461/119‑1 (MQ=255)
                                    cccGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGcccc                                                                                                                                     >  2:405461/1‑119 (MQ=255)
                                        tGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCg                                                                                                  <  1:538750/150‑1 (MQ=255)
                                                          cTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAg                                                                                >  2:135985/1‑150 (MQ=255)
                                                                    gtaggggtGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAgg                                                                      >  2:361069/1‑150 (MQ=255)
                                                                          gtGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCCACTGCAGGAACTcc                                                                >  2:206124/1‑150 (MQ=255)
                                                                           tGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTg                                                                                     >  1:413824/1‑128 (MQ=255)
                                                                           tGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTg                                                                                     <  2:413824/128‑1 (MQ=255)
                                                                                   cGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCctgctg                                                           <  2:241612/146‑1 (MQ=255)
                                                                                   cGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCctgctg                                                           >  1:241612/1‑146 (MQ=255)
                                                                                    gTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCt                                                      >  2:506802/1‑150 (MQ=255)
                                                                                      gACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTcc                                                    <  1:135985/150‑1 (MQ=255)
                                                                                         ttGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGa                                                 >  2:345533/1‑150 (MQ=255)
                                                                                         ttGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGa                                                 <  1:345533/150‑1 (MQ=255)
                                                                                             ggggCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGACTTGGTAAGAGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGt                                             >  2:244392/1‑150 (MQ=255)
                                                                                              gggCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGtt                                            <  2:482329/150‑1 (MQ=255)
                                                                                                  gcgcATGAACAGTTGGAACTGTTTCCGCCCCAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGACCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCgg                                        <  1:226314/150‑1 (MQ=255)
                                                                                                        gAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGc                                  >  1:747797/1‑150 (MQ=255)
                                                                                                         aaCAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTg                                                                                                                  >  2:316017/1‑69 (MQ=255)
                                                                                                         aaCAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTg                                                                                                                  <  1:316017/69‑1 (MQ=255)
                                                                                                          aCAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTc                                >  1:410450/1‑150 (MQ=255)
                                                                                                             gTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGAt                             >  2:213276/1‑150 (MQ=255)
                                                                                                                 gAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCt                                 >  1:681145/1‑142 (MQ=255)
                                                                                                                 gAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCt                                 <  2:681145/142‑1 (MQ=255)
                                                                                                                  aGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGc                        >  1:120363/1‑150 (MQ=255)
                                                                                                                   gCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGct                       <  2:120363/150‑1 (MQ=255)
                                                                                                                           cGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCa               <  2:412193/150‑1 (MQ=255)
                                                                                                                                   tGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTg       <  2:747797/150‑1 (MQ=255)
                                                                                                                                   tGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTg       <  1:361069/150‑1 (MQ=255)
                                                                                                                                     aaaGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGc     >  1:438256/1‑150 (MQ=255)
                                                                                                                                        ggggCTGCATTGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTc  >  1:157995/1‑150 (MQ=255)
                                                                                                                                        ggggCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCAt              >  1:283783/1‑138 (MQ=255)
                                                                                                                                        ggggCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCAt              <  2:283783/138‑1 (MQ=255)
                                                                                                                                        ggggCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCt    <  2:649136/148‑1 (MQ=255)
                                                                                                                                        ggggCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCt    >  1:649136/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTC  >  NC_002947/1382187‑1382472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑