| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,509,444 | C→A | 14.8% | Q106H (CAG→CAT) | PP_1324 ← | endonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,509,444 | 0 | C | A | 14.8% | 64.1 / 3.8 | 27 | Q106H (CAG→CAT) | PP_1324 | endonuclease |
| Reads supporting (aligned to +/- strand): ref base C (12/11); new base A (2/2); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 | |||||||||||
GGTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCC > NC_002947/1509303‑1509576 | ggTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGc < 2:745927/150‑1 (MQ=255) gTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGAGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCg > 2:20259/1‑150 (MQ=255) gACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTTCATGGTGGCTACCCTGCAGGGCGAc < 1:20259/150‑1 (MQ=255) ggCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAggg < 1:617661/150‑1 (MQ=255) cgcgCAACAACGACCAGCCCGCGATACACAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGcccc < 1:565355/150‑1 (MQ=255) cGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGcctc > 1:744425/1‑150 (MQ=255) ccAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGAAGTAGTTGCCCCAGTGGGCCTCCtt < 1:212389/150‑1 (MQ=255) cAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTc > 1:190602/1‑113 (MQ=255) cAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTc < 2:190602/113‑1 (MQ=255) gATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGTGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGACTCCTTCTGCAggca > 2:162410/1‑148 (MQ=255) cATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAg > 1:189909/1‑89 (MQ=255) cATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAg < 2:189909/89‑1 (MQ=255) gagCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTGTCCTGCAGGGCGACCACGTCGAAGAGGCAGGGGCAGTTGCCCCATGGGGCCTCCTTCTGCAGGAACAGGGTgg > 1:730583/1‑150 (MQ=255) aaaCGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCAGGGTAGCCACCATGCAg < 1:436115/76‑1 (MQ=37) aaaCGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCAGGGTAGCCACCATGCAg > 2:436115/1‑76 (MQ=37) ttCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCTGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTggc < 2:747089/130‑1 (MQ=255) ttCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTggc > 1:747089/1‑130 (MQ=255) gCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCg > 1:113431/1‑150 (MQ=255) gCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCga < 2:744425/150‑1 (MQ=255) gTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGATCAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTc < 1:302247/136‑1 (MQ=255) gTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTc > 2:302247/1‑136 (MQ=255) tgcctgcATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCTCCCGTCGAGACTGTAGAGAGAGCCTcagtaga > 2:498479/1‑144 (MQ=255) cctgcATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGCCGAGAGCGCCTCCGAAGccc > 2:210462/1‑150 (MQ=255) ctgcATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGt > 1:560015/1‑104 (MQ=255) ctgcATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGt < 2:560015/104‑1 (MQ=255) ctgcAGGGTAGCCACCATGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCAcc < 1:575539/98‑1 (MQ=255) ctgcAGGGTAGCCACCATGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCAcc > 2:575539/1‑98 (MQ=255) | GGTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCC > NC_002947/1509303‑1509576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |