Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,522,888 C→T 12.1% R252C (CGT→TGT)  ftsW → lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,522,8880CT12.1% 82.3 / 4.1 33R252C (CGT→TGT) ftsWlipid II flippase
Reads supporting (aligned to +/- strand):  ref base C (15/14);  new base T (2/2);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

TGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTGGGCGTGGTCGGCTCGCT  >  NC_002947/1522742‑1523009
                                                                                                                                                  |                                                                                                                         
tGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTg                                                                                                                        >  1:370077/1‑150 (MQ=255)
 gATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTgg                                                                                                                       <  2:370077/150‑1 (MQ=255)
                  tgctggcggtATTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTg                                                                                                      <  1:657757/150‑1 (MQ=255)
                     tggcggtATTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGcc                                                                                                   >  1:540498/1‑150 (MQ=255)
                        cggtATTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTTGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTgg                                                                                                <  2:170719/150‑1 (MQ=255)
                            aTTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCaa                                                                                            >  1:298668/1‑150 (MQ=255)
                                gTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGc                                                                                        <  1:27875/150‑1 (MQ=255)
                                   cTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTg                                                                                     <  1:184867/150‑1 (MQ=255)
                                                    cTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTAcct                                                                    <  1:85481/150‑1 (MQ=255)
                                                        cGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTAcctgcct                                                                >  1:55663/1‑150 (MQ=255)
                                                                    cTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTggtt                                                                                                              >  2:460005/1‑90 (MQ=255)
                                                                    cTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTggtt                                                                                                              <  1:460005/92‑3 (MQ=255)
                                                                       attaCGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGCGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGAc                                                 <  2:540498/150‑1 (MQ=255)
                                                                        ttaCGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTg                                                                                                                 <  2:695351/85‑1 (MQ=255)
                                                                        ttaCGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTg                                                                                                                 >  1:695351/1‑85 (MQ=255)
                                                                                 ccGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTtct                                       >  2:426710/1‑150 (MQ=255)
                                                                                      cccTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTc                                  >  2:330058/1‑150 (MQ=255)
                                                                                                     ttCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTAcctgc                                                                  <  1:491979/103‑1 (MQ=255)
                                                                                                     ttCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTAcctgc                                                                  >  2:491979/1‑103 (MQ=255)
                                                                                                      tCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTgg                  <  2:55663/150‑1 (MQ=255)
                                                                                                              ggCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTGGGCGTGGTc          >  1:103600/1‑150 (MQ=255)
                                                                                                                 tACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGTAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTGGGCGTGGTCGGc       >  2:244898/1‑150 (MQ=255)
                                                                                                                     aGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTGGGCGTg             <  1:60749/140‑1 (MQ=255)
                                                                                                                     aGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTGGGCGTg             >  2:60749/1‑140 (MQ=255)
                                                                                                                      gCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTGGGCGTGGTCGGCTCgct  >  2:714637/1‑150 (MQ=255)
                                                                                                                         gACCCAGGCACTGATCGCCTTCGGCTGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCgtg                                           <  2:268957/106‑1 (MQ=255)
                                                                                                                         gACCCAGGCACTGATCGCCTTCGGCTGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCgtg                                           <  2:125903/106‑1 (MQ=255)
                                                                                                                         gACCCAGGCACTGATCGCCTTCGGCTGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCgtg                                           >  1:268957/1‑106 (MQ=255)
                                                                                                                         gACCCAGGCACTGATCGCCTTCGGCTGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCgtg                                           >  1:125903/1‑106 (MQ=255)
                                                                                                                          aCCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTc                                              >  2:429722/1‑102 (MQ=255)
                                                                                                                          aCCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTc                                              <  1:429722/102‑1 (MQ=255)
                                                                                                                            ccAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCacac                                                     <  2:279028/93‑1 (MQ=255)
                                                                                                                            ccAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCacac                                                     >  1:279028/1‑93 (MQ=255)
                                                                                                                                                  |                                                                                                                         
TGATGGTGGTGCTGGCGGTGCTGGCGGTATTTGTCCTGGTTCAGGCGCAGCCCTACCGGATGGCGCGCCTTATTACGTTCACCGACCCCTGGGCCGATCAGTTCGGCTCCGGCTACCAGCTGACCCAGGCACTGATCGCCTTCGGCCGTGGCGAGTGGCTGGGCGTTGGCCTGGGCAACAGCGTGCAGAAGCAGTTCTACCTGCCTGAAGCGCACACCGACTTCGTGTTCTCGGTCCTGGCGGAAGAACTGGGCGTGGTCGGCTCGCT  >  NC_002947/1522742‑1523009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑