Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,592,029 T→G 21.9% intergenic (‑94/‑109) PP_1395 ← / → PP_1396 AraC family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,592,0290TG21.9% 70.6 / 19.4 41intergenic (‑94/‑109)PP_1395/PP_1396AraC family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/17);  new base G (4/5);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGC  >  NC_002947/1591880‑1592169
                                                                                                                                                     |                                                                                                                                            
gCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCCGCATGTGGATTGCCTGATCCTGCCGCGCt                                                                                                                                              <  2:268439/150‑1 (MQ=255)
   aggtCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCa                                                                                                                                           >  1:751299/4‑150 (MQ=255)
   aGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCa                                                                                                                                           <  2:751299/150‑1 (MQ=255)
      tCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGa                                                                                                                                        <  2:619784/150‑1 (MQ=255)
           gcgcTGCAGTTCCAGGGTCGGTTCGGTGGACGTAGCGGTGTTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATCGCCTGATCCTGCCGCGCTGCAGGATCAgg                                                                                                                                   <  1:228690/150‑1 (MQ=255)
                   gTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGTATCAGGCAATCGAc                                                                                                                           <  2:742357/150‑1 (MQ=255)
                     tCCCGGGTCGGTTCGGTGGGCGTGGCCGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACAt                                                                                                                         <  1:151288/150‑1 (MQ=255)
                        aGGGTCTGTTCGGTGGACGTGGCGGTGGTCATGATGGCTGTCCTCTGCTGACCGACGCAGCATAGGTTTCCGCAAAGACGGGCGCTAGTCTAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGATCAGGCAATCCACATGCa                                                                                                                      <  1:239356/150‑1 (MQ=37)
                                ttcggtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATATGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGATCAg                                                                                                                                    >  2:649849/1‑128 (MQ=255)
                                ttcggtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATATGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGATCAg                                                                                                                                    <  1:649849/128‑1 (MQ=255)
                                 tcggtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGcc                                                                                                             >  2:292321/1‑150 (MQ=255)
                                    gtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTaa                                                                                                          <  1:654299/150‑1 (MQ=255)
                                                    tCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGa                                                                                          >  1:552622/1‑150 (MQ=255)
                                                    tCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGa                                                                                          <  2:516513/150‑1 (MQ=255)
                                                                   tctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGc                                                                           >  2:661493/1‑150 (MQ=255)
                                                                   tctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCACAGCCTTGGc                                                                           >  1:31940/1‑150 (MQ=255)
                                                                   tctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGGGAGCGCCTAACCTTGGc                                                                           >  2:27007/1‑150 (MQ=255)
                                                                          aCCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTgg                                                                    >  1:211039/1‑150 (MQ=255)
                                                                               cGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCtttt                                                               <  1:292321/150‑1 (MQ=255)
                                                                                         gTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTa                                                                                   <  1:372200/120‑1 (MQ=255)
                                                                                         gTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTa                                                                                   >  2:372200/1‑120 (MQ=255)
                                                                                          tttGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCgg                                                                                              >  2:9043/1‑108 (MQ=255)
                                                                                          tttGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCgg                                                                                              <  1:9043/108‑1 (MQ=255)
                                                                                          tttGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTg                                                    >  1:100163/1‑150 (MQ=255)
                                                                                           ttGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGc                                                   <  1:661493/150‑1 (MQ=255)
                                                                                                          cgcTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGAAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATtgtg                                    <  2:552622/150‑1 (MQ=255)
                                                                                                           gcTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGc                                   <  2:211039/150‑1 (MQ=255)
                                                                                                             tAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAg                                             <  2:524418/138‑1 (MQ=255)
                                                                                                             tAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAg                                             >  1:524418/1‑138 (MQ=255)
                                                                                                                        tGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTaaa                      <  2:100163/150‑1 (MQ=255)
                                                                                                                             gtgGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTCCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAAcc                 >  1:648938/1‑150 (MQ=255)
                                                                                                                              tgGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCg                <  2:599486/150‑1 (MQ=255)
                                                                                                                                     ccTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGc                                                   >  2:404630/1‑108 (MQ=255)
                                                                                                                                     ccTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGc                                                   <  1:404630/108‑1 (MQ=255)
                                                                                                                                      cTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGCCCCGTAAACAACCGGTcaccca        >  1:228172/1‑150 (MQ=255)
                                                                                                                                      cTGATCCTGCAGCGCGGGAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGc                                                       >  2:385143/1‑103 (MQ=255)
                                                                                                                                      cTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGc                                                       <  1:385143/103‑1 (MQ=255)
                                                                                                                                        gATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccacc      >  2:579325/1‑150 (MQ=255)
                                                                                                                                        gATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacccacc      >  2:583011/1‑150 (MQ=255)
                                                                                                                                            cTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGc  <  2:228172/150‑1 (MQ=255)
                                                                                                                                                 gcgcTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGc                                   >  2:142758/1‑112 (MQ=255)
                                                                                                                                                 gcgcTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGc                                   <  1:142758/112‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
GCCAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTGC  >  NC_002947/1591880‑1592169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑