Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,970,236 G→A 8.7% L188L (CTG→CTA)  mtnA → methylthioribose‑1‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,970,2360GA 8.7% 123.1 / 3.5 46L188L (CTG→CTA) mtnAmethylthioribose‑1‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

GCCAACCTGACCATGGCCCAGCAGGGTATCGAGCTGATCCGTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGCCGACTCGGCGCTGGCCCACCTCATGAAGACCAAGGGCA  >  NC_002947/1970090‑1970384
                                                                                                                                                  |                                                                                                                                                    
cccAACCTGACCATGGCCCAGCAGGGTATCGAGCTGATCCGTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGaa                                                                                                                                                   <  2:220309/149‑1 (MQ=255)
              ggCCCAGCAGGGTATCGAGCTGATCCGTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGATCg                                                                                                                                     <  1:751827/150‑1 (MQ=255)
                    gcagGGTATCGAGCTGATCCGTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTa                                                                                                                               <  1:69688/150‑1 (MQ=255)
                           aTCGAGCTGATCCGTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCgct                                                                                                                        <  2:735844/150‑3 (MQ=255)
                                   gATCCGTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCATACCGGGGACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCg                                                                                                                <  1:511674/150‑1 (MQ=255)
                                        gTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTAGAAGGCATGGTCGa                                                                                                                                        <  1:78330/121‑1 (MQ=255)
                                        gTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTAGAAGGCATGGTCGa                                                                                                                                        >  2:78330/1‑121 (MQ=255)
                                        gTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTAGAAGGCATGGTCGa                                                                                                                                        >  2:243794/1‑121 (MQ=255)
                                        gTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTAGAAGGCATGGTCGa                                                                                                                                        <  1:243794/121‑1 (MQ=255)
                                         tCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGccc                                                                                                             >  2:147361/1‑147 (MQ=255)
                                         tCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGccc                                                                                                             <  1:147361/147‑1 (MQ=255)
                                              aTCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCgg                                                                                                                         >  1:311649/1‑130 (MQ=255)
                                              aTCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCgg                                                                                                                         <  2:311649/130‑1 (MQ=255)
                                                 aGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAgg                                                                                                  >  1:626687/1‑150 (MQ=255)
                                                   ggCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATg                                                                                                                                             >  2:155105/1‑105 (MQ=255)
                                                   ggCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATg                                                                                                                                             <  1:155105/105‑1 (MQ=255)
                                                   ggCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTgg                                                                                                         <  1:149979/141‑1 (MQ=255)
                                                   ggCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTgg                                                                                                         >  2:149979/1‑141 (MQ=255)
                                                         gCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCgcgc                                                                                          <  2:1978/150‑1 (MQ=255)
                                                            cAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTCGGCTGCGTGGCTCGCGCCTg                                                                                       >  2:173304/1‑150 (MQ=255)
                                                                   tGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCt                                                                                >  1:315563/1‑150 (MQ=255)
                                                                        aCCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAg                                                                           >  1:358718/1‑150 (MQ=255)
                                                                           ttCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTggg                                                                             >  2:188168/1‑145 (MQ=255)
                                                                           ttCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTggg                                                                             <  1:187974/145‑1 (MQ=255)
                                                                           ttCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTggg                                                                             >  2:187974/1‑145 (MQ=255)
                                                                           ttCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTggg                                                                             <  1:188168/145‑1 (MQ=255)
                                                                                          gcCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATc                                                         >  1:403774/1‑150 (MQ=255)
                                                                                                     tggtggCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATTGGCGAGCGGGTTTATGCAGCAGATACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCt                                              <  1:355372/150‑1 (MQ=255)
                                                                                                         ggCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTtgt                                          <  1:224253/150‑1 (MQ=255)
                                                                                                           cTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGTCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAgg                                                              <  2:481597/128‑1 (MQ=255)
                                                                                                           cTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGTCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAgg                                                              >  1:481597/1‑128 (MQ=255)
                                                                                                            tttGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGcc                                       >  2:193699/1‑150 (MQ=255)
                                                                                                                       aCTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCaa                                                                   >  1:463376/1‑111 (MQ=255)
                                                                                                                       aCTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCaa                                                                   <  2:463376/111‑1 (MQ=255)
                                                                                                                       aCTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGCCGACTCGGCGCt                            >  2:152279/1‑150 (MQ=255)
                                                                                                                           ggggTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGa                                                                                      <  1:178683/88‑1 (MQ=255)
                                                                                                                           ggggTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGa                                                                                      >  2:178683/1‑88 (MQ=255)
                                                                                                                              gTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGCCGACTCGGCGCTGGCCCAc                     <  2:739725/150‑1 (MQ=255)
                                                                                                                                 aTCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCTCGCCTGACCGGCTGGGAGCt                                                                         <  1:75965/95‑1 (MQ=255)
                                                                                                                                 aTCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCTCGCCTGACCGGCTGGGAGCt                                                                         >  2:75965/1‑95 (MQ=255)
                                                                                                                                             tACCTGGAAGGCATGGTCGAGCGGGGTTATGCCGGTGAAACTCGCCCTTGGCTGCAGGGCTCGCGCCTAACCGGCTGGGCGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGCCGACTCGGCGCTGGCCCCCCTCATGAAGACCAAg      >  1:428584/1‑150 (MQ=255)
                                                                                                                                               gcTGGAAGGCATGGTCGCGCTGGTTTATGCGGGTGAGACTCGCCCTTGTCTGCAGGGCTCGGGCCTGACCGGCTGTGAGCTGGCCAACGAGGGCATCCCGGTGAGCCTTTGTGCCGACTCGGCGCTGGCTAACCTCATAAGGACCAAggg    <  2:366079/149‑1 (MQ=255)
                                                                                                                                                cTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCg                                                      >  2:294429/1‑99 (MQ=255)
                                                                                                                                                cTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCg                                                      <  1:294429/99‑1 (MQ=255)
                                                                                                                                                 tGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGCCGACTCGGCGCTGGCCCACCTCATGAAGACCAAGGGCa  >  1:72754/1‑150 (MQ=255)
                                                                                                                                                 tGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGCCGACTCGGCGCTGGCCCACCTCATGAAGACCAAGGGCa  >  2:549836/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCAACCTGACCATGGCCCAGCAGGGTATCGAGCTGATCCGTCGCCATCAGGGCAATGCCCAGGCCCTGCTCACCTTCGGCAACGCCGGCGCCCTTGCCAGTGGTGGCTTTGGCACCGCACTGGGGGTGATCCGTGCCGGCTACCTGGAAGGCATGGTCGAGCGGGTTTATGCCGGTGAGACTCGCCCTTGGCTGCAGGGCTCGCGCCTGACCGGCTGGGAGCTGGCCAACGAGGGCATCCCGGTGACCCTTTGTGCCGACTCGGCGCTGGCCCACCTCATGAAGACCAAGGGCA  >  NC_002947/1970090‑1970384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑