Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,103,203	C→G	15.6%	noncoding (35/76 nt)	PP_t24 →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,103,203	0	C	G	15.6%	80.0 / 8.0	32	noncoding (35/76 nt)	PP_t24	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (13/14);  new base G (3/2);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

CGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCC  >  NC_002947/2103063‑2103349                                                                                                                                             |                                                                                                                                                   cGAGTCTCGTTTCCCGCTGCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCGTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                                                           <  2:391064/150‑1 (MQ=255)    gTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACTCTGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                                                                        <  1:51200/150‑1 (MQ=255)     tctcGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                                                                       >  2:628900/1‑150 (MQ=255)                            aTACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                                                                          >  2:341906/1‑124 (MQ=255)                            aTACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                                                                          <  1:341906/124‑1 (MQ=255)                             tACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGc                                                                                                               >  1:725762/1‑150 (MQ=255)                                       tCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCaa                                                                                                     >  1:623246/1‑150 (MQ=255)                                               gTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACa                                                                                             >  1:254763/1‑150 (MQ=255)                                               gTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACa                                                                                             >  1:331696/1‑150 (MQ=255)                                                tGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAg                                                                                            <  2:331696/150‑1 (MQ=255)                                                  cAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgt                                                                                                                                           <  1:392877/101‑2 (MQ=255)                                                  cAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgt                                                                                                                                           >  2:392877/1‑100 (MQ=255)                                                                                                  cTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGa                                                                                                   <  1:492349/93‑1 (MQ=255)                                                                                                  cTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGa                                                                                                   >  2:492349/1‑93 (MQ=255)                                                                                                  cTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGTCGTCttt                                          >  1:616433/1‑150 (MQ=255)                                                                                                  cTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCCTCGGCGTCttt                                          >  2:306994/1‑150 (MQ=255)                                                                                                        aatGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTGGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTtttcgt                                    <  1:49471/148‑1 (MQ=255)                                                                                                         ttGCGGGAGCAGTTCAGTTGGTAGAGCACGCACTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGGTTCCCGCGCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTtttcgtt                                   <  2:272798/150‑1 (MQ=25)                                                                                                              ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGt                              <  2:360053/150‑1 (MQ=255)                                                                                                                      tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCa                      >  2:674884/1‑150 (MQ=255)                                                                                                                         gTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCCTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCg                   <  1:306994/150‑1 (MQ=255)                                                                                                                             gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGt                                                                              <  1:697674/87‑1 (MQ=255)                                                                                                                             gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGt                                                                              >  2:697674/1‑87 (MQ=255)                                                                                                                                 accccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCAt           >  2:411232/5‑150 (MQ=255)                                                                                                                                  gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATg          <  2:277169/150‑1 (MQ=255)                                                                                                                                   cccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTtttc                                      <  2:343183/119‑1 (MQ=255)                                                                                                                                   cccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTtttc                                      >  1:343183/3‑121 (MQ=255)                                                                                                                                   cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATgg         <  2:725762/150‑1 (MQ=255)                                                                                                                                      gACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGc                                                      >  2:519828/1‑102 (MQ=255)                                                                                                                                      gACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGc                                                      <  1:519828/102‑1 (MQ=255)                                                                                                                                          ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATccc  <  2:254763/150‑1 (MQ=255)                                                                                                                                          ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATccc  >  1:658396/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                                   CGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCC  >  NC_002947/2103063‑2103349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑