| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,135,562 | G→A | 10.0% | A411V (GCG→GTG) | fadE ← | medium‑long chain acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,135,562 | 0 | G | A | 10.0% | 101.4 / 3.6 | 40 | A411V (GCG→GTG) | fadE | medium‑long chain acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (21/15); new base A (2/2); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01 | |||||||||||
GGTGGTACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGTTCATCAACATCATCCAGCCCTTGCCGA > NC_002947/2135422‑2135709 | ggtggtACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCa < 2:666523/150‑1 (MQ=255) tggtACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCACCAATGGCACg > 1:361768/1‑150 (MQ=255) gtACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGCAGGCCGCCAATGGCACGtt < 1:436268/150‑1 (MQ=255) ttGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAAc > 2:326939/1‑150 (MQ=255) ggATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCTGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCaa < 2:552859/133‑1 (MQ=255) ggATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCaa > 1:552859/1‑133 (MQ=255) cGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAAGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTa > 1:530970/1‑150 (MQ=255) gAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACCGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCAGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTAc > 2:480261/1‑150 (MQ=255) gCTTTTCGCCCATGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGc < 2:354683/150‑1 (MQ=255) ttttCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCg > 2:637836/1‑150 (MQ=255) ttttCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCACCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCg < 2:361768/150‑1 (MQ=255) ttttCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCAGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCg > 2:637758/1‑150 (MQ=255) cccAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCAcc > 2:141694/1‑150 (MQ=255) ccAGGTCCACGGCCTTGGCGGTCCGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCa < 2:488450/150‑1 (MQ=255) ccAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCa > 2:146204/1‑150 (MQ=255) cACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCACCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGgt > 2:430925/1‑150 (MQ=255) gCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTggcg > 1:435686/1‑150 (MQ=255) cagGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGt > 1:475365/1‑150 (MQ=255) gggCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGa < 1:601107/150‑1 (MQ=255) tGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGGCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGAccgccg > 1:646023/1‑150 (MQ=255) tGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGAccgccg > 1:251042/1‑150 (MQ=255) aTCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGt < 1:146204/150‑1 (MQ=255) cAGCCAGGCGTTGCCGCAAATGCGTTCCAGCGATTCCTGTATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTgc < 1:401931/150‑2 (MQ=255) gCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAAGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAg < 2:530970/150‑1 (MQ=255) ccgccAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGAGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGccc > 1:12428/1‑150 (MQ=255) cGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCACCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAgg < 1:430925/150‑1 (MQ=255) cAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGAccgcc > 2:264673/1‑115 (MQ=255) cAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGAccgcc < 1:264673/115‑1 (MQ=255) cAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCAGCCCGCCGGAAGTGAGATGGAGCGGCCCACCGACAGGCAGtt > 2:528869/1‑150 (MQ=255) ttCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGTTCATCaa < 1:141694/150‑1 (MQ=255) cTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTg > 1:78216/1‑76 (MQ=255) cTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTg < 2:78216/76‑1 (MQ=255) tGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGTTCATCAAcatc > 2:392795/1‑150 (MQ=255) cTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGt < 2:254499/132‑1 (MQ=255) cTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGt > 1:254499/1‑132 (MQ=255) cTTCGAAGGCCGCCAATGGCACGTTGAACAGATAGCGGATAGTTGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGCGCGGCCCACCGACAGGCAGTTCGTCAACATCATCCAGc < 2:435686/150‑1 (MQ=255) ttCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAg < 1:504080/112‑1 (MQ=255) ttCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGTTCATCAACATCATCCAGcc > 2:701505/1‑150 (MQ=255) ttCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCACTGAGATGGAg > 2:504080/1‑112 (MQ=255) gAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGTTCATCAACATCATCCAGCCCtt > 1:306762/1‑150 (MQ=255) ccgccAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGACACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGTTCATCAACATCATCCAGCCCTTGCCGa > 2:244461/1‑150 (MQ=255) | GGTGGTACTTGAGGATCGCCGACAGCACCGAGGGCTTTTCGCCCAGGTCCACGGCCTTGGCGGTCAGCAGGCGGGCACTGTCCATCAGCCAGGCGTTGCCGCCAATGCGTGCCAGCGATTCCTGGATGCCTTCGAAGGCCGCCAATGGCACGTTGAACTGCTCGCGGATATTGGCGTACTGGCCGGTCACCAGGCTGGTGTACTTGGCGGCACCGGTGCCGACCGCCGGCAGTGAGATGGAGCGGCCCACCGACAGGCAGTTCATCAACATCATCCAGCCCTTGCCGA > NC_002947/2135422‑2135709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |