Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,154,439 C→A 9.7% G126W (GGG→TGG)  PP_1909 ← septum formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,154,4390CA 9.7% 121.1 / 2.6 41G126W (GGG→TGG) PP_1909septum formation protein
Reads supporting (aligned to +/- strand):  ref base C (21/16);  new base A (2/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

TCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTAT  >  NC_002947/2154292‑2154583
                                                                                                                                                   |                                                                                                                                                
tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                                >  2:100165/1‑150 (MQ=255)
tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                                >  2:238318/1‑150 (MQ=255)
 ctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGt                                                                                                                                               <  2:175638/150‑1 (MQ=255)
            tCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGt                                                                                                                                               >  2:668820/1‑139 (MQ=255)
            tCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGt                                                                                                                                               <  1:668820/139‑1 (MQ=255)
                ccccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagcag                                                                                                                                >  2:582219/1‑150 (MQ=255)
                 cccAGCCCCTCCGCCTTTAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                               <  1:1997/150‑1 (MQ=255)
                                ttGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGa                                                                                                                >  1:329551/1‑150 (MQ=255)
                                  gAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                              <  2:517175/150‑1 (MQ=255)
                                         ccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACATGGCATTGCCCGGTGGCGCTGTTAAGCAGTGCCAGCCCGGTAAGAAAGCTCAcc                                                                                                       <  2:246321/150‑1 (MQ=255)
                                          cTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACct                                                                                                      >  1:683199/1‑150 (MQ=255)
                                          cTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACct                                                                                                      >  2:233519/1‑150 (MQ=255)
                                           tGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgg                                                                                                                                             >  2:45681/1‑110 (MQ=255)
                                           tGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgg                                                                                                                                             <  1:45681/110‑1 (MQ=255)
                                                       cGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCa                                                                                         <  1:100165/150‑1 (MQ=255)
                                                        ggCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                              >  2:686901/1‑128 (MQ=255)
                                                        ggCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                              <  1:686901/128‑1 (MQ=255)
                                                                              gCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgc                                                                  >  2:12407/1‑150 (MQ=255)
                                                                                   aCCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAACTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCg                                                             >  2:504842/1‑150 (MQ=255)
                                                                                        ctccctgctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGcc                                                                                                                            <  2:79625/82‑1 (MQ=255)
                                                                                        ctccctgctcAGGTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGcc                                                                                                                            >  1:79625/1‑82 (MQ=255)
                                                                                           cctgctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATg                                                     >  2:74032/1‑150 (MQ=255)
                                                                                           cctgctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATg                                                     <  2:416889/150‑1 (MQ=255)
                                                                                             tgctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCaa                                                                      >  1:652039/1‑131 (MQ=255)
                                                                                             tgctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCGCGCaa                                                                      <  2:652039/131‑1 (MQ=255)
                                                                                                       aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                            >  2:430853/1‑147 (MQ=255)
                                                                                                       aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                            <  1:430853/147‑1 (MQ=255)
                                                                                                        cGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGc                                        <  1:628203/150‑1 (MQ=255)
                                                                                                         gCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCa                                       <  1:582219/150‑1 (MQ=255)
                                                                                                              gtCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGc                                  >  1:15537/1‑150 (MQ=255)
                                                                                                               tCACGGTAAAAGGCACGCAATCAACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                                    <  1:728326/115‑1 (MQ=255)
                                                                                                               tCACGGTAAAAGGCACGCAATCAACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                                    >  2:728326/1‑115 (MQ=255)
                                                                                                                 aCGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCg                               >  1:109034/1‑150 (MQ=255)
                                                                                                                         aGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAc                       >  2:345358/1‑150 (MQ=255)
                                                                                                                               gCAATCCACCTGGCATTGCACGGTAGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAAc                 >  1:16927/1‑150 (MQ=255)
                                                                                                                                 aaTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCt               >  1:667401/1‑150 (MQ=255)
                                                                                                                                    ccACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGc                    <  2:579758/142‑1 (MQ=255)
                                                                                                                                    ccACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGc                    >  1:579758/1‑142 (MQ=255)
                                                                                                                                     cACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTc           <  1:233519/150‑1 (MQ=255)
                                                                                                                                          ggCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGc      <  2:683199/150‑1 (MQ=255)
                                                                                                                                              ttGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTAt  >  1:484530/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTAT  >  NC_002947/2154292‑2154583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑