| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,458,114 | C→G | 16.7% | intergenic (+143/‑62) | PP_2150 → / → sthA | thiamine biosynthesis lipoprotein/pyridine nucleotide transhydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,458,114 | 0 | C | G | 16.7% | 59.3 / 6.9 | 24 | intergenic (+143/‑62) | PP_2150/sthA | thiamine biosynthesis lipoprotein/pyridine nucleotide transhydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (12/8); new base G (2/2); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GAATGGGTGAAGCATATGTAGTGCGGGCAAAATTGGCCTACGACGCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTCCGGCCCGGCCGGTGAAGGCGCGGCAATGAAC > NC_002947/2458001‑2458244 | gAATGGGTGAAGCATATGTAGTGCGGGCAAAATTGGCCTACGACGCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGCCGCCGGGGCGCCATGag > 2:558281/1‑124 (MQ=255)gAATGGGTGAAGCATATGTAGTGCGGGCAAAATTGGCCTACGACGCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGCCGCCGGGGCGCCATGag < 1:558281/125‑2 (MQ=255) tGAAGCATATGTAGTGCGGGCAAAATTGGCCTACGACGCGACCAAGGGTTAATGTGGGCGGCGGTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGcc > 2:255631/1‑150 (MQ=255) atGTAGTGCGGGCAAAATTGGCCTACGACGCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGa > 1:202158/1‑150 (MQ=255) ggCCTACGACGCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTc < 1:291176/150‑1 (MQ=255) acgacgCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGCCGCCGGGGCGCCATGaga < 1:196761/87‑3 (MQ=38) acgacgCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGCCGCCGGGGCGCCATGaga > 2:196761/1‑85 (MQ=38) gACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTacgac > 2:574924/1‑150 (MQ=255) gTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCgcga < 1:466425/83‑1 (MQ=255) gTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCgcga > 2:466425/1‑83 (MQ=255) tgtgCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCa < 2:625950/117‑1 (MQ=255) tgtgCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCa > 1:625950/1‑117 (MQ=255) ttCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCg < 1:297516/74‑1 (MQ=255) ttCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCg > 2:297516/1‑74 (MQ=255) cAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTccggcccggc > 2:526564/1‑150 (MQ=255) ggTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTCCGGCccggccgg > 2:695690/1‑150 (MQ=255) ttAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTCCGGCccggccg < 2:259464/143‑1 (MQ=255) ttAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTCCGGCccggccg > 1:259464/1‑143 (MQ=255) tAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTccggccc < 2:309199/137‑1 (MQ=255) tAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTccggccc > 1:309199/1‑137 (MQ=255) gACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTCCGGCCCGGCCGGTGAAGgcgc < 1:526564/150‑1 (MQ=255) aCCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCt < 1:146747/95‑1 (MQ=255) aCCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCt > 2:146747/1‑95 (MQ=255) gAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTCCGGCCCGGCCGGTGAAGGCGCGGCAATGAAc > 2:602917/1‑150 (MQ=255) | GAATGGGTGAAGCATATGTAGTGCGGGCAAAATTGGCCTACGACGCGACCAAGGGTTAATGTGCGCGGCGTTCAGGGTTGATTAGACTGCACCCGAATTTTCTCATGGCGCCCCGGCGGCATGATCGTGCCCCGCGAGCGACCGTGGCTTGCGGGCCAGTTCTGAAGGAGTACGCATGGCTGTCTACAACTACGACGTAGTGGTGCTGGGTTCCGGCCCGGCCGGTGAAGGCGCGGCAATGAAC > NC_002947/2458001‑2458244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |