| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,490,773 | C→G | 12.9% | G51G (GGC→GGG) | PP_2185 → | formate dehydrogenase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,490,773 | 0 | C | G | 12.9% | 84.0 / 4.8 | 31 | G51G (GGC→GGG) | PP_2185 | formate dehydrogenase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (12/15); new base G (2/2); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
TTCGACCCCGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGAAGGCATGGTGGTGCGCACCGAAACCCC > NC_002947/2490633‑2490922 | ttcGACCCCGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATc < 1:86707/150‑1 (MQ=255) gACCCCGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATcccc < 1:135104/150‑1 (MQ=255) cccGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGTGCCCAGCAtgg < 1:74817/145‑3 (MQ=255) cccGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGTGCCCAGCAtgg > 2:74817/1‑143 (MQ=255) ccGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGTGCCCAGCAtg < 1:708688/143‑2 (MQ=255) ccGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGTGCCCAGCAtg > 2:708688/1‑142 (MQ=255) cTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACtgt < 2:365497/150‑1 (MQ=255) tCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTgc < 2:412549/150‑1 (MQ=255) aTAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCa > 2:540352/1‑150 (MQ=255) aaaaGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCAcc > 2:80779/1‑150 (MQ=255) cTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGaa > 1:431415/1‑150 (MQ=255) tCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAAc < 1:352892/126‑1 (MQ=255) tCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAAc > 2:352892/1‑126 (MQ=255) cccGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCgg > 1:21419/1‑150 (MQ=255) cAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCAtggtg < 2:15294/150‑1 (MQ=255) cTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTTTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGaa > 2:488726/1‑150 (MQ=255) gACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATgcgc < 1:80779/150‑1 (MQ=255) gACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATgcgc < 2:398179/150‑1 (MQ=255) aCGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGcgcg < 1:201183/150‑1 (MQ=255) tgctgGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGcaccac > 2:722412/1‑150 (MQ=255) ggCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCa < 2:431415/150‑1 (MQ=255) cTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGaa > 1:77152/1‑70 (MQ=255) cTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGaa < 2:77152/70‑1 (MQ=255) tCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGc > 2:527783/1‑150 (MQ=255) gTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGaa < 2:21419/150‑1 (MQ=255) cgcgcCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGAAGGCAtg < 1:540352/150‑1 (MQ=255) gcgcCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGAAGGCAtgg > 1:626172/1‑150 (MQ=255) cgccATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGAAGGCAtggtg > 2:313272/1‑145 (MQ=255) cgccATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGAAGGCAtggtg < 1:313272/145‑1 (MQ=255) tGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGaa > 2:233913/1‑128 (MQ=255) tGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGaa < 1:233913/128‑1 (MQ=255) cACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGAAGGCATGGTGGTGCGCACCGAAAcccc > 2:443027/1‑150 (MQ=255) | TTCGACCCCGCTTCTGATAAAAGCAGCGACCTCGGCACCCCGGCCCGCGAAAGCGACGTGCAGGTCAGCCTGAGCATCGACGGCCGCGCCATCAGCGTGCCTGCTGGCACCTCGGTCATGCGCGCCGCCGCCATGCTGGGCACCAGCATCCCCAAACTGTGCGCCACCGACAGCCTCGAAGCCTTCGGCTCCTGCCGCATGTGCATGGTGGAAATCGAAGGCATGCGCGGCTACCCGGCATCCTGCACCACGCCAGTCAGCGAAGGCATGGTGGTGCGCACCGAAACCCC > NC_002947/2490633‑2490922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |