| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,633,500 | T→G | 17.4% | intergenic (+100/‑82) | hupB → / → PP_2304 | DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,633,500 | 0 | T | G | 17.4% | 40.3 / 5.9 | 23 | intergenic (+100/‑82) | hupB/PP_2304 | DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D |
| Reads supporting (aligned to +/- strand): ref base T (9/10); new base G (2/2); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
AGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATC > NC_002947/2633358‑2633635 | aGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATgc < 2:678915/150‑1 (MQ=255) tGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTa < 1:379805/150‑1 (MQ=255) aaCTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCgg > 1:582062/1‑150 (MQ=255) tcgtcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGa > 2:286776/1‑150 (MQ=255) gtcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATc < 1:292033/118‑1 (MQ=255) gtcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATc > 2:292033/1‑118 (MQ=255) cAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTgg < 2:582062/150‑1 (MQ=255) ctcGGACCAGGCCAGGCCGGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCa > 1:217267/3‑111 (MQ=255) ctcGGACCAGGCCAGGCCGGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCa < 2:217267/109‑1 (MQ=255) ggcctggcctGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGtgt > 2:610924/1‑148 (MQ=255) ctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATgcgc > 1:467436/1‑81 (MQ=255) ctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATgcgc < 2:467436/81‑1 (MQ=255) gcAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCa < 2:118596/142‑1 (MQ=255) gcAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCa > 1:118596/1‑142 (MQ=255) aaaCGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGGCTCAGTGGTACAGCCGTTCCTGGGGGACGCATGCTGCAAAATATCAGGGACAAttt > 1:384544/1‑149 (MQ=255) aaCGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACTCTCCGCGGTTGTCGCTGCACTGGTACCGCCGTCTTTGGGCGACGCACTCTGCTAAATATCAGGGACAATTca < 2:650069/150‑1 (MQ=255) ttCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAg > 2:280827/1‑150 (MQ=255) caggtgtGCTAAGAAAAGGCGCATCCTCGGATGGGCCTTTCTTTTATCAGGATTCTACCCGCGCTCCGCGGTTGTCCACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGcc < 2:299891/144‑1 (MQ=255) gAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTc < 2:142525/64‑1 (MQ=255) gAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTc > 1:142525/1‑64 (MQ=255) gAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCatcatc < 1:280827/150‑1 (MQ=255) gcgcATCCGAGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgg > 2:262157/1‑83 (MQ=255) gcgcATCCGAGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgg < 1:262157/83‑1 (MQ=255) | AGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATC > NC_002947/2633358‑2633635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |