| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,788,121 | T→A | 16.0% | V134D (GTC→GAC) | ahpF → | alkyl hydroperoxide reductase subunit F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,788,121 | 0 | T | A | 16.0% | 58.6 / 5.7 | 25 | V134D (GTC→GAC) | ahpF | alkyl hydroperoxide reductase subunit F |
| Reads supporting (aligned to +/- strand): ref base T (10/11); new base A (2/2); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
GTTCACATCGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTA > NC_002947/2787975‑2788241 | gTTCACATCGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAATGCAGTTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGAcgtcgt > 1:7727/1‑150 (MQ=255) ttGGTGCGGGCACTGCTGCAAGTGGGTGGCCACCCGGCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTg < 2:41293/150‑1 (MQ=255) tGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGa < 2:562245/150‑1 (MQ=255) ctgcAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACt > 2:507413/1‑134 (MQ=255) ctgcAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACt < 1:507413/134‑1 (MQ=255) gtgggtggCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAAc < 1:722989/150‑1 (MQ=255) ccACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCTGGACGACGTCCGGGCagttc < 2:85889/122‑5 (MQ=255) ccACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCTGGACGACGTCCGGGCagttc > 1:85889/1‑118 (MQ=255) cACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCTGGACGACGTCCGGGCagt < 1:192578/119‑3 (MQ=255) cACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCTGGACGACGTCCGGGCagt > 2:192578/1‑117 (MQ=255) aCCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTgc > 1:120978/1‑150 (MQ=255) gAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGt > 1:715647/1‑150 (MQ=255) aGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCACGGAAGTCGTACAGGCACTACACCTGATGGCGGTCCTCCTCCCCAACTGGCTCCATATGGCCATCGACGGTGCGCTGTTcc < 2:7727/150‑1 (MQ=255) gCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCa < 2:715647/150‑1 (MQ=255) gATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGa < 2:120978/150‑1 (MQ=255) aGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAg > 2:424304/1‑150 (MQ=255) ggAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATc < 1:412648/150‑1 (MQ=255) aaTTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGa > 1:82348/1‑150 (MQ=255) ttCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATc > 2:676486/1‑150 (MQ=255) tCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTAc > 1:670648/1‑78 (MQ=255) tCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTAc < 2:670648/78‑1 (MQ=255) cTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGc > 1:37998/1‑150 (MQ=255) aCCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCg > 1:208183/1‑150 (MQ=255) ctcGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTa < 2:208183/150‑1 (MQ=255) aaCTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTg > 2:116613/1‑99 (MQ=255) aaCTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTg < 1:116613/99‑1 (MQ=255) | GTTCACATCGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTA > NC_002947/2787975‑2788241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |