| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,790,201 | G→T | 20.2% | S30R (AGC→AGA) | PP_2442 ← | DUF480 domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,790,201 | 0 | G | T | 20.2% | 31.7 / 5.1 | 20 | S30R (AGC→AGA) | PP_2442 | DUF480 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (8/8); new base T (2/2); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01 | |||||||||||
GGTCATGCCCTGACCTTCGAGGGCGCGCAAGGCCTGGCCGACCTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTGGGGAAAGGCCACTAGGGTAGGTAATGCCATCGCGT > NC_002947/2790066‑2790343 | ggTCATGCCCTGACCTTCGAGGGCGCGCAAGGCCTGGCCGACCTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCtt < 2:229423/150‑1 (MQ=255) gcAAGGCCTGGCCGACCTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCCTTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCCATCCGCGAGCCGAGAACGCGGa > 1:243891/1‑150 (MQ=255) cGACCTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGTCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGt > 1:457837/1‑150 (MQ=255) cGACCTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGATTTTAAAACAACGAGCCGAGAACGCGGATCTCGATGCTGt > 2:248937/1‑150 (MQ=255) ccTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCggg > 2:173885/1‑95 (MQ=255) ccTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCggg < 1:173885/95‑1 (MQ=255) cTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGaa > 1:458541/1‑150 (MQ=255) cTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGc > 2:466787/1‑150 (MQ=255) aGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCAccc < 1:248937/150‑1 (MQ=255) ggCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGCCGCTGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCgtgt < 1:39325/150‑1 (MQ=255) tGGTCTTCTGGTTGCCGGCCCGGTAACGTCGATTGGGGTTCAGCTGTTATCTTTCCGTGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATgg < 2:457837/150‑1 (MQ=255) ggTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGc > 2:41472/1‑150 (MQ=255) tGCCGGCCAGGGCCAGGGCAGGGAGGGTCAGCGGGGAGCTTTGCGGGCGGGTGGCCTGCTTTTCGATCAGCGGGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTCACATGGCCCTGTGCCGTg < 2:243891/150‑1 (MQ=255) tGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTg < 1:41472/150‑1 (MQ=255) gCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTgg > 2:202507/1‑150 (MQ=255) gCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGTCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTgg > 1:50179/1‑150 (MQ=255) gCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGTCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTgg > 1:50146/1‑150 (MQ=255) gggCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTGGGGAAAGGCCa < 1:513848/150‑1 (MQ=255) tGAGGGTCAGCGGGTAGCTTTCCGGTCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTGGGGAAAGGCCACTAggg < 2:50146/150‑1 (MQ=255) tGAGGGTCAGCGGGTAGCTTTCCGGTCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTGGGGAAAGGCCACTAggg < 2:50179/150‑1 (MQ=255) cAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGCACGGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTGGGGAAAGGCCACTAGGGTAGGTaa < 1:466787/150‑1 (MQ=255) tttCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTATCGTGGCTGGGGAAAGGCCACTAGGGTAGGTAATGCCATCGCGt > 1:36851/1‑150 (MQ=255) | GGTCATGCCCTGACCTTCGAGGGCGCGCAAGGCCTGGCCGACCTGGCCTTGGGTGAGGTTCATCACCGGCTCGCGGCTGGTCTTCTGGTTGCAGGCCAGGACCAGGGCATTGAGGGTCAGCGGGTAGCTTTCCGGGCTGGTGGCCTGCTTTTCGATCAGCGAGCCGAGAACGCGGATCTCGATGCTGTTGAAGCGGCCTTCACCCGCGGTTTCGTGTTCTGACATGGCCCTGTCTCGTGGCTGGGGAAAGGCCACTAGGGTAGGTAATGCCATCGCGT > NC_002947/2790066‑2790343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |