| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,859,193 | T→C | 14.3% | C127C (TGT→TGC) ‡ | PP_2511 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,859,193 | 0 | T | C | 14.3% | 65.5 / 4.1 | 28 | C127C (TGT→TGC) ‡ | PP_2511 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (11/13); new base C (2/2); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01 | |||||||||||
GATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGCAACGGGTATGC > NC_002947/2859062‑2859341 | gATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCaaa < 2:602455/150‑1 (MQ=255) gggCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGCTTGAGGCCACACTTGTCCGGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTca < 1:467591/150‑1 (MQ=255) cTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTgag > 1:342948/1‑132 (MQ=255) cTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTgag < 2:342948/132‑1 (MQ=255) cgcgCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGcc < 2:590675/150‑1 (MQ=255) gcgcCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCg > 2:119114/1‑150 (MQ=255) cACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTACAACGGTgc < 2:597171/118‑2 (MQ=255) cACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTACAACGGTgc > 1:597171/1‑117 (MQ=255) gCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGt < 2:526320/106‑1 (MQ=255) gCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGt > 1:526320/1‑106 (MQ=255) cGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCt > 1:388174/1‑150 (MQ=255) aCGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTc > 2:426194/1‑150 (MQ=255) cTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTg > 1:489177/1‑150 (MQ=255) tGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAgg > 1:138839/1‑150 (MQ=255) tGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTc < 1:426194/150‑1 (MQ=255) tCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATCAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTg < 2:489177/150‑1 (MQ=255) gATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGa < 2:388174/150‑1 (MQ=255) aGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACAccc > 1:280811/1‑150 (MQ=255) gCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACAccccc > 2:672135/1‑150 (MQ=255) gCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTACAACGGTgcc > 1:677788/1‑53 (MQ=255) gCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTACAACGGTgcc < 2:677788/55‑3 (MQ=38) tACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATcc > 1:464767/1‑83 (MQ=255) tACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATcc < 2:464767/83‑1 (MQ=255) acaTCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATg < 2:37963/150‑1 (MQ=255) ggCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTtgt < 1:672135/150‑1 (MQ=255) cGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGAt < 2:388618/130‑1 (MQ=255) cGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGAt > 1:388618/1‑130 (MQ=255) gTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGCAACGGGTATGc < 1:119114/150‑1 (MQ=255) | GATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCTGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCCGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGAAAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGCAACGGGTATGC > NC_002947/2859062‑2859341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |