Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 195,559 C→A 16.7% T355T (ACC→ACA)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,5590CA16.7% 107.2 / 11.9 42T355T (ACC→ACA) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (19/16);  new base A (3/4);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAG  >  NC_002947/195420‑195702
                                                                                                                                           |                                                                                                                                               
cGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAAtt                                                                                                                                       >  1:322831/1‑150 (MQ=35)
     accgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg                                                                                                                                  >  1:327192/1‑150 (MQ=35)
      ccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGc                                                                                                                                 >  1:706895/1‑150 (MQ=37)
             ccTGTCCAATGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGAc                                                                                                                          >  1:649253/1‑150 (MQ=255)
             ccTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGa                                                                                                                           >  2:446874/1‑149 (MQ=255)
             ccTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGa                                                                                                                           <  1:446874/149‑1 (MQ=255)
                    aaCGGTCAGGTCATAACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGATCCCGGCCAATGACGTCTACAACAACTGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGCTAATTTCGAGCAACTTACCCCGAAt                                                                                                                   <  2:86262/150‑1 (MQ=14)
                           aGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGa                                                                                                                                   <  2:334253/127‑1 (MQ=37)
                           aGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGa                                                                                                                                   >  1:334253/1‑127 (MQ=35)
                            ggTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGAcccc                                                                                                           <  2:322831/150‑1 (MQ=255)
                                       ttGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCa                                                                                                >  1:465608/1‑150 (MQ=38)
                                                      cccAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCgg                                                                                 >  2:296436/1‑150 (MQ=35)
                                                      cccAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGGTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCgg                                                                                 <  1:462330/150‑1 (MQ=25)
                                                            gCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGaca                                                                           <  1:135748/150‑1 (MQ=38)
                                                                          cAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACAACGAACGACACGGTCGACACCACCACGGCGAcc                                                             >  2:22049/1‑150 (MQ=14)
                                                                           aGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAccc                                                            <  2:545580/150‑1 (MQ=255)
                                                                               cccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGAc                                                        >  2:47854/1‑150 (MQ=40)
                                                                                 cGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACgg                                                      >  2:133824/1‑150 (MQ=255)
                                                                                  ggCCAATGACTTCTACAACAACGGTCCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGc                                                     <  1:22049/150‑1 (MQ=255)
                                                                                    cgaaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGAGACCCTGACGGCGa                                                   <  2:218565/148‑1 (MQ=2)
                                                                                    ccAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACTCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCCCCGCGACCCTGACGGCGa                                                   <  2:639679/150‑1 (MQ=255)
                                                                                      aaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGc                                                 >  1:247334/1‑150 (MQ=39)
                                                                                      aaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGc                                                 <  1:133824/150‑1 (MQ=38)
                                                                                                 caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAc                                      >  2:624579/1‑150 (MQ=38)
                                                                                                 caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAc                                      >  2:650287/1‑150 (MQ=12)
                                                                                                  aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAcc                                     >  1:735757/1‑150 (MQ=12)
                                                                                                   acaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCg                                    >  2:338510/1‑150 (MQ=38)
                                                                                                   acaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCg                                    <  1:673583/150‑1 (MQ=12)
                                                                                                    caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGa                                   >  2:578454/1‑150 (MQ=39)
                                                                                                     aacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGCGCAACTGACACCGAATCCGACCCCGGCTCAGACCACGATCAACTTCTCGGTCGATACCACCACCTCGACCCTGACGGCGAGCCCGTCGGTCACCGaa                                  <  2:649253/150‑1 (MQ=11)
                                                                                                     aacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGaa                                  <  2:327192/150‑1 (MQ=37)
                                                                                                              aCCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg                         <  2:247334/150‑1 (MQ=40)
                                                                                                                cGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCGGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGAt                       >  1:603824/1‑150 (MQ=255)
                                                                                                                cGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGAt                       <  1:650287/150‑1 (MQ=16)
                                                                                                                 gTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGGCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc                      >  2:512173/1‑150 (MQ=255)
                                                                                                                            cATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGt           >  1:410906/1‑150 (MQ=255)
                                                                                                                            cATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACGCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGt           >  2:401592/1‑150 (MQ=255)
                                                                                                                            cATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAAACCGACCCCGGCTCAGACCACGATCAACGAATCGGTCGACACCACCACCGCGCACAGTACTGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGt           <  1:583667/150‑1 (MQ=11)
                                                                                                                             aTCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                          >  1:290802/1‑134 (MQ=40)
                                                                                                                             aTCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                          <  2:290802/134‑1 (MQ=40)
                                                                                                                                    aCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa   <  2:735757/150‑1 (MQ=12)
                                                                                                                                     cGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAg  <  1:296436/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAG  >  NC_002947/195420‑195702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑