| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,146,657 | G→A | 30.1% | intergenic (+50/+16) | PP_2761 → / ← sfnA | ribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,146,657 | 0 | G | A | 30.1% | 14.9 / 11.7 | 20 | intergenic (+50/+16) | PP_2761/sfnA | ribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein |
| Reads supporting (aligned to +/- strand): ref base G (8/6); new base A (3/3); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01 | |||||||||||
TCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGT > NC_002947/3146525‑3146797 | tctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTc < 1:644822/150‑1 (MQ=255) ctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCa < 1:568331/150‑1 (MQ=255) ggTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCtt < 2:703400/150‑1 (MQ=255) tgctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGggcg > 2:13621/1‑150 (MQ=255) ctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACCCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGc > 1:446714/1‑114 (MQ=255) ctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACCCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGc < 2:446714/114‑1 (MQ=255) ccAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGGCATTGa > 2:294275/1‑150 (MQ=255) gcgcTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCga > 1:281839/1‑150 (MQ=255) tAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGt < 2:281839/150‑1 (MQ=255) cTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGTCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCtt > 1:364782/1‑150 (MQ=255) tGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAg > 2:532217/1‑150 (MQ=255) aCGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTa > 2:32317/1‑150 (MQ=255) gCCTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa < 1:292980/117‑1 (MQ=255) gCCTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa > 2:292980/1‑117 (MQ=255) ccTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCt > 2:148916/1‑77 (MQ=255) ccTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCt > 2:523773/1‑77 (MQ=255) ccTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCt < 1:523773/77‑1 (MQ=255) ccTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCt < 1:148916/77‑1 (MQ=255) cTGGGGTATGCACCAGGCCATACGTTCTGAACTTGTGTGCACCGCCACAGATCTGCCCTGCAT‑TGCCTGTTCTGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGt < 1:13621/150‑1 (MQ=255) aTGTCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCcatacat > 2:614732/1‑56 (MQ=255) | TCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTC‑GCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGT > NC_002947/3146525‑3146797 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |