Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,726,458	T→G	14.5%	F356L (TTT→TTG)	PP_3291 →	metallo‑beta‑lactamase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,726,458	0	T	G	14.5%	56.6 / 6.3	29	F356L (TTT→TTG)	PP_3291	metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (13/11);  new base G (2/2);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

CGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATG  >  NC_002947/3726317‑3726601                                                                                                                                              |                                                                                                                                                cGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCgg                                                                                                                                         <  1:77053/150‑1 (MQ=255)          cAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGAcgcg                                                                                                                                >  1:739532/1‑150 (MQ=255)             gCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCaa                                                                                                                                              <  2:547475/133‑1 (MQ=255)             gCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCaa                                                                                                                                              >  1:547475/1‑133 (MQ=255)             gCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCgggg                                                                                                                             >  1:557276/1‑150 (MQ=255)                     aCCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGata                                                                                                                     <  1:667003/150‑1 (MQ=255)                       cAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATAtc                                                                                                                   >  2:305361/1‑150 (MQ=255)                          cTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCCCCGGCTTGAAAGCCggagaa                                                                                                                                     >  1:297169/1‑125 (MQ=255)                          cTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCCCCGGCTTGAAAGCCggagaa                                                                                                                                     <  2:297169/129‑5 (MQ=255)                                gAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggg                                                                                                                                        <  1:416268/120‑1 (MQ=255)                                gAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCggg                                                                                                                                        >  2:416268/1‑120 (MQ=255)                                  gCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGATTCACCCACTTGAGGCACTGACCCGAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGgcgc                                                                                                        >  1:467710/1‑150 (MQ=255)                                     cGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTCCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCca                                                                                                     <  1:394638/150‑1 (MQ=255)                                                     gccAGCCGTATGGCGACCGGCGCACGGGTGCTTCACCGCCTGAAGGCACTGGCCCCAAACCCGCGCAAAAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGGGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCAc                                                                                     <  2:557276/150‑1 (MQ=255)                                                        aGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGc                                                                                  >  2:662475/1‑150 (MQ=255)                                                                                  gCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGt                                                        <  2:116708/150‑1 (MQ=255)                                                                                                       ggCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTTCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTc                                   <  2:102865/150‑1 (MQ=255)                                                                                                           ccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGc                                                                                             <  2:17504/88‑1 (MQ=255)                                                                                                           ccAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGc                                                                                             >  1:17504/1‑88 (MQ=255)                                                                                                                              ttCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGaaa            >  1:405955/1‑150 (MQ=255)                                                                                                                                cTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATc          >  2:421203/1‑150 (MQ=255)                                                                                                                                 tGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAgtgt                                                                                               >  1:641961/1‑64 (MQ=255)                                                                                                                                 tGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAgtgt                                                                                               <  2:641961/64‑1 (MQ=255)                                                                                                                                   ttCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATg       >  1:692213/1‑150 (MQ=255)                                                                                                                                    cccccGGCTTGAAAGCCGGAGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTTTGCGTATGCCGATGCTAATGAAAGCATgg      >  2:282933/4‑150 (MQ=255)                                                                                                                                      tcCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGc                          >  1:561057/1‑128 (MQ=255)                                                                                                                                      tcCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGc                          <  2:561057/128‑1 (MQ=255)                                                                                                                                       ccGGCTTGAAAGCCGGAGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAAt   <  1:282933/150‑1 (MQ=255)                                                                                                                                        cGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCTCACGCCGATGCTGATGAAATCATGGAATg  <  2:262628/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                CGATGAGTCCAGGCACCTGGACCAGCTGCGCGAGCCCGCAGTGATCATCGCCGCCAGCGGCATGGCGACCGGCGGACGGGTGCTTCACCACCTGAAGGCACTGGCCCCAAACCCGCGCAACAGCATTCTGTTCTCCGGCTTTCAAGCCGGGGGGACGCGGGGTGCCGATATCGTCGCGGGCGCCCACAGTGTGCGGTTGCACGGCGAGGATGTGCCTATCCGTGCACAGGTGCATGCGATGGAGAATCTTTCTGCGCACGCCGATGCTGATGAAATCATGGAATG  >  NC_002947/3726317‑3726601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑