| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,785,536 | G→T | 13.9% | A230S (GCT→TCT) | PP_3348 → | GGDEF domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,785,536 | 0 | G | T | 13.9% | 66.5 / 2.0 | 27 | A230S (GCT→TCT) | PP_3348 | GGDEF domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (11/12); new base T (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
AAAGCCTGTCCTCGGCATTGACGACGGCCTGGCGCCTGAAACACGAAATGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAGGTGCTGCAGCGCATCGGTGCAGCGATCA > NC_002947/3785402‑3785680 | aaaGCCTGTCCTCGGCATTGACGACGGCCTGGCGCCTGAAACACGAAATGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACg > 2:328539/1‑150 (MQ=255) ccTGTCCTCGGCATTGACGACGGCCTGGCGCCTGAAACACGAAATGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCg < 2:726307/150‑1 (MQ=255) tCGGCATTGACGACGGCCTGGCGCCTGAAACACGAAATGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAgg < 1:683742/150‑1 (MQ=255) tGACGACGGCCTGGCGCCTGAAACACGAAATGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCgg < 2:671180/150‑1 (MQ=255) acacGAAATGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTAGATTAAAGGCCGTGTGGag > 2:417562/1‑149 (MQ=255) aaaTGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTgg > 1:389101/1‑150 (MQ=255) gCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGt < 2:478305/150‑1 (MQ=255) gcgcATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCAt > 2:251081/1‑150 (MQ=255) cATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCaa < 2:75592/150‑1 (MQ=255) ccTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATg < 2:412609/101‑1 (MQ=255) ccTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATg > 1:412609/1‑101 (MQ=255) ggACGAACTGACCGGCCTGAAAAACCGACGTGCTTTTTTCGACAATGCTTCGGCTCAGAACGTCGGGTACAGGGATGCGGGTACATTCATGGCTGTGTTGATGCTGGACGTTGACCATTTCAAACAGATCCACGGCCCATTCGGGCATga > 2:322915/1‑149 (MQ=255) aCCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGAc > 2:40625/1‑150 (MQ=255) cGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCa > 1:169095/1‑150 (MQ=255) ggCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAg < 1:40625/150‑1 (MQ=255) cTGAACAACCGACGTGCTTTTTTCGACAATTCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGAc < 2:21488/144‑1 (MQ=255) cTGAACAACCGACGTGCTTTTTTCGACAATTCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGAc < 2:21605/144‑1 (MQ=255) cTGAACAACCGACGTGCTTTTTTCGACAATTCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGAc > 1:21605/1‑144 (MQ=255) cTGAACAACCGACGTGCTTTTTTCGACAATTCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGAc > 1:21488/1‑144 (MQ=255) aacaacCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATg > 1:42260/1‑84 (MQ=255) aacaacCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATg < 2:42260/84‑1 (MQ=255) aacaacCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAGGtgctg < 1:328539/150‑1 (MQ=255) acaacCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAGGtgctgc > 1:182381/1‑150 (MQ=255) caacCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAGGTGCTGCa < 1:251081/150‑1 (MQ=255) ttttttCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAgg > 2:315585/1‑131 (MQ=255) ttttttCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAgg < 1:315585/131‑1 (MQ=255) aCAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAGGTGCTGCAGCGCATCGGTGCAGCGATCa < 1:417562/150‑1 (MQ=255) | AAAGCCTGTCCTCGGCATTGACGACGGCCTGGCGCCTGAAACACGAAATGCAACGCGCGCATCGGCTGGCCATGGTGGCCTCGTGCACGGACGAACTGACCGGCCTGAACAACCGACGTGCTTTTTTCGACAATGCTTCGGCGCAGCACGTCCGGTGCAGGGATGCGGGTACATTCATGGCCGTGTTGATGCTGGACGTCGACCATTTCAAACAGATCAACGACACGTTCGGGCATGCCGCAGGCGACCAGGTGCTGCAGCGCATCGGTGCAGCGATCA > NC_002947/3785402‑3785680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |