| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,881,873 | A→T | 16.0% | Q163L (CAG→CTG) | oprN → | multidrug RND transporter outer membrane protein OprN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,881,873 | 0 | A | T | 16.0% | 57.7 / 5.6 | 25 | Q163L (CAG→CTG) | oprN | multidrug RND transporter outer membrane protein OprN |
| Reads supporting (aligned to +/- strand): ref base A (8/13); new base T (2/2); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GATCCCCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGAT > NC_002947/3881724‑3882009 | gATCCCCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACa < 2:374244/150‑1 (MQ=255) tCCCCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGt > 2:682105/1‑150 (MQ=255) cccGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTg < 1:735735/150‑1 (MQ=255) ccGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGc > 1:210509/1‑150 (MQ=255) cGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGAt < 2:604675/150‑1 (MQ=255) ggTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGa > 1:54145/1‑150 (MQ=255) gagcgCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGAt < 2:54145/150‑1 (MQ=255) gagcgCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGAt < 1:518808/150‑1 (MQ=255) cTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTa < 2:210509/150‑1 (MQ=255) cGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACgg < 1:101916/150‑1 (MQ=255) gACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGt < 1:682105/150‑1 (MQ=255) gACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGt < 2:408560/150‑1 (MQ=255) aTCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCTATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAAc > 2:439119/1‑150 (MQ=255) tCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTGCGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAAcc > 2:207952/1‑150 (MQ=255) tCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAAcc < 1:137914/150‑1 (MQ=255) aaGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAAcc > 1:423472/1‑135 (MQ=255) aaGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAAcc < 2:423472/135‑1 (MQ=255) aaGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTgg < 1:69686/111‑1 (MQ=255) aaGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTgg > 2:69686/1‑111 (MQ=255) cGGTTGCGGCCGCCGACCTGCAACAGTTGAAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACTGGCAACTGCGCTTTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAACACCCAGCAGGAATCACGCACCATTACCGaaa < 1:126682/150‑1 (MQ=255) ccgccgccgACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCTATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTgc < 1:439119/150‑1 (MQ=255) ccgccgccgACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTgc > 1:274905/1‑150 (MQ=255) gctgACCTGCAACTGTTGCAGGTCGGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTgc > 2:100494/4‑79 (MQ=38) gctgACCTGCAACTGTTGCAGGTCGGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTgc < 1:100494/76‑1 (MQ=38) tgACCTGCAACTGTTGCAGGTCGGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGAt < 1:406777/147‑1 (MQ=255) tgACCTGCAACTGTTGCAGGTCGGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGAt > 2:406777/2‑148 (MQ=255) | GATCCCCGGCCAGACCACCGAGCGGGTCAATAGCGAGCGCTACGACCTGGGCCTGGACATGGCCTGGGAGCTTGACCTGTTCGGGCGCATCCAGCGCCAGATCGAAGCCAGCGAAGCCCAGGAAGCGGTTGCCGCCGCCGACCTGCAACAGTTGCAGGTCAGCCTGATCGCCGAACTGGTCGATGCCTACGGGCAACTGCGCGGTGCCCAGTTGCGCGAAAAGATCGCCCTGGCCAACCTGAAGACCCAGCAGGAATCACGCACCATTACCGAAACCCTGCGCGAT > NC_002947/3881724‑3882009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |