| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,996,944 | A→C | 19.3% | M88R (ATG→AGG) | PP_3525 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,996,944 | 0 | A | C | 19.3% | 50.7 / 13.2 | 32 | M88R (ATG→AGG) | PP_3525 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base C (3/3); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 | |||||||||||
CACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTAGGCCT > NC_002947/3996799‑3997091 | cACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGCCGGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgcg > 1:383864/1‑150 (MQ=255) tgctgcAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATg > 1:338867/1‑150 (MQ=255) gTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg > 1:735890/1‑146 (MQ=255) gTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg < 2:735890/146‑1 (MQ=255) cAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGaa > 1:29148/1‑150 (MQ=255) aCTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATAACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAg > 1:429515/1‑150 (MQ=255) cccTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCt < 1:257359/150‑1 (MQ=255) cctcctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAgcggc > 2:255541/1‑142 (MQ=255) cctcctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAgcggc < 1:255541/142‑1 (MQ=255) cctcctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCtt > 2:633608/1‑150 (MQ=255) cccGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTGCGCTGat < 1:289051/113‑2 (MQ=255) cccGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTGCGCTGat > 2:289051/1‑112 (MQ=255) cACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCa > 2:79619/1‑128 (MQ=255) cACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCa < 1:79619/128‑1 (MQ=255) cGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGt < 2:460478/150‑1 (MQ=255) gCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAg > 2:592709/1‑128 (MQ=255) gCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAg < 1:592709/128‑1 (MQ=255) gAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGTTGATCAGCGCATGCCTGCGCTGatc > 2:142300/1‑76 (MQ=38) gAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGTTGATCAGCGCATGCCTGCGCTGatc < 1:142300/78‑3 (MQ=38) gggCCGGTACCGCGAACAGTTTGATGCTCGGCGCAGGCGTGCGTGGAATGTCGTCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGccacca < 2:383864/149‑1 (MQ=255) gCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGt > 2:191150/1‑150 (MQ=255) ccGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTa < 1:191150/150‑1 (MQ=255) cgcgAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTg > 2:479176/1‑150 (MQ=255) gAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCAACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCgt > 1:207719/1‑150 (MQ=255) tGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCAc < 2:429515/150‑1 (MQ=255) tGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCaa > 2:651383/1‑150 (MQ=255) gATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAg < 2:612785/150‑1 (MQ=255) tCAGCGCATGCCTGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAg > 2:268198/1‑59 (MQ=38) tCAGCGCATGCCTGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAg < 1:268198/59‑1 (MQ=38) cAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTATAATCTGCGTGTAGGCACCAAGGCt < 2:29148/150‑1 (MQ=255) gcgcAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGt < 1:633608/150‑1 (MQ=255) gCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTAGGCCt > 1:575902/1‑150 (MQ=255) | CACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTAGGCCT > NC_002947/3996799‑3997091 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |