| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,120,082 | G→A | 19.0% | A152T (GCG→ACG) | adhB → | alcohol dehydrogenase cytochrome c subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,120,082 | 0 | G | A | 19.0% | 38.5 / 4.6 | 21 | A152T (GCG→ACG) | adhB | alcohol dehydrogenase cytochrome c subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/11); new base A (2/2); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01 | |||||||||||
ACTTCGACCGCGCCGTGCGCCAGGGTGTCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATAT > NC_002947/4119942‑4120227 | aCTTCGACCGCGCCGTGCGCCAGGGTGTCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAgg < 2:425590/150‑1 (MQ=255) ccGCGCCGTGCTCCAGGGTGTCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCt < 1:148454/150‑1 (MQ=255) cgcgCCGTGCGCCAGGGTGTCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTg < 2:229499/150‑1 (MQ=255) gcCGTGCGCCAGGGTGTCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGc > 2:657182/1‑150 (MQ=255) gtgtCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCt < 1:398792/150‑1 (MQ=255) tCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGc < 1:171624/150‑1 (MQ=255) gcCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCt < 2:563353/150‑1 (MQ=255) aCGGCTGTACCCGGCGCTGGACTCCCTGTCGTACGCCAATCTCAGCGATGATGATGTCAAGTCTTTTTATCCTTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTg < 1:203193/150‑1 (MQ=255) tGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCa > 2:414103/1‑150 (MQ=255) ccGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCACGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGcc < 2:527233/150‑1 (MQ=255) ccGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTg < 1:211733/150‑1 (MQ=255) ccGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTg > 1:18946/1‑150 (MQ=255) ccAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCa > 1:120441/1‑150 (MQ=255) gTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGACGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAg > 1:301756/1‑150 (MQ=255) gTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGACGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAg > 1:301712/1‑150 (MQ=255) tGTATGCGTTCTTCATGCACGGCGTGCAACCGACGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAgg < 2:301712/150‑1 (MQ=255) tGTATGCGTTCTTCATGCACGGCGTGCAACCGACGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAgg < 2:301756/150‑1 (MQ=255) cATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGaa < 1:414103/150‑1 (MQ=255) tGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAAcc > 1:267596/1‑150 (MQ=255) cACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTCGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGt < 1:657182/150‑1 (MQ=255) aCCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTatat > 1:630554/1‑150 (MQ=255) | ACTTCGACCGCGCCGTGCGCCAGGGTGTCGCGCCGGGTGGCCGACGGCTGTACCCGGCGATGCCTTACCCGTCCTACGCCAAGCTCAGCGATGATGATGTCAAGGCGCTGTATGCGTTCTTCATGCACGGCGTGCAACCGGCGCGGCAGGCCAACCTGGGCAGCGACATCCCCTGGCCGCTGAACCTGCGCTGGCCCATCGCCTTGTGGAACGGCCTGTTCGCCGCCACTACGCCTTACACCGACAAGGCCGGGCAGGATGCACAGTGGAACCGTGGCGCCTATAT > NC_002947/4119942‑4120227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |