| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,155,338 | A→G | 12.9% | F67S (TTC→TCC) | PP_3658 ← | aromatic compound MFS transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,155,338 | 0 | A | G | 12.9% | 70.6 / 3.6 | 31 | F67S (TTC→TCC) | PP_3658 | aromatic compound MFS transporter |
| Reads supporting (aligned to +/- strand): ref base A (18/9); new base G (2/2); total (20/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
ATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCAGAATAGAATAAGTCCATGA > NC_002947/4155194‑4155484 | aTGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAAt > 2:722279/1‑150 (MQ=255) tGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAAAGCGTTGTGCCAATGTGCCTAAAAGAATGGCGCCCAGCATTGTCCCGAACAATGCTgcgc > 1:123263/1‑150 (MQ=255) aCCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCTCAGCATTGTTCGGGACAATGCTgggc > 2:362692/1‑123 (MQ=255) aCCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCTCAGCATTGTTCGGGACAATGCTGggc < 1:362692/126‑1 (MQ=255) ccggCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCg > 1:749469/4‑150 (MQ=255) gAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGc < 2:55022/135‑1 (MQ=255) gAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGc > 1:55022/1‑135 (MQ=255) aaCGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTTCGGGACAATGCTgg < 2:490826/105‑2 (MQ=255) aaCGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTTCGGGACAATGCTgg > 1:490826/1‑104 (MQ=255) aaCGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGc > 1:35738/1‑150 (MQ=255) aaCAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGaa > 2:723324/1‑89 (MQ=255) aaCAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGaa < 1:723324/89‑1 (MQ=255) gATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCccac > 1:561392/1‑150 (MQ=255) cAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGCCGTAGCCATCGATAATCAg > 2:495609/1‑150 (MQ=255) aTGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCTATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGaa < 1:722279/150‑1 (MQ=255) cTAAAAAAATAGCGCCCAGCATTGTCCCGAACACTGCTGAGCCAGCCATTACCCCTGCGCTGGTTGCGTCACTTTGAATATGAAAGAGGCGGCGTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAaca > 1:545465/1‑150 (MQ=255) tAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAacac > 1:319261/1‑150 (MQ=255) aaaaTAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCAc < 2:634584/150‑1 (MQ=255) aGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCagaa < 1:495609/150‑1 (MQ=255) gcgcCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCagaat > 2:494177/1‑150 (MQ=255) ccAGCATTGTCCCGACCAATGCGGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGa < 1:269732/128‑1 (MQ=255) ccAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGAtgg > 2:302603/1‑78 (MQ=255) ccAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGAtgg < 1:302603/78‑1 (MQ=255) ccAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGa > 2:269732/1‑128 (MQ=255) cAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCagaatagaat < 2:35738/150‑1 (MQ=255) cAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCagaatagaat > 1:31649/1‑150 (MQ=255) gCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCAGAATAGAATaa > 2:547802/1‑150 (MQ=255) tCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCAGAATAGAATAAGTCCAt > 1:107446/1‑150 (MQ=255) ccGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCagaa < 2:360967/134‑1 (MQ=255) ccGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCagaa > 1:360967/1‑134 (MQ=255) cagAACAATGATGAGCAAGCCATTAACCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCGCACCACTGCCAAGTAGTAGCCATCGATAATCAGAATAAACACGCACCAGAATAGAATAAGTACATGa > 2:219381/3‑150 (MQ=255) gAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTa < 2:42414/99‑1 (MQ=255) gAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTa > 1:42414/1‑99 (MQ=255) | ATGATACTGAAGGTCAGCGGGCTGTCAGTGAACCCTGCCGCAGCGGTGAAAACGCTGAACAGCGCTACACAGATCGCGATCATTTTCGGCCGGCCAATTCGATCTGCCAATGTGCCTAAAAAAATAGCGCCCAGCATTGTCCCGAACAATGCTGAGCCAGCCATTACCCCGGCGCTGGTTGCGTCAATTTGCATATCGGCCATGATGGCTGGCAGGGCAGCCCCCACCACTGCCAAGTCGTAGCCATCGATAATCAGAATCAACACGCACCAGAATAGAATAAGTCCATGA > NC_002947/4155194‑4155484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |