| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,252,246 | G→C | 28.5% | intergenic (‑170/+70) | PP_3726 ← / ← rocE | enoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,252,246 | 0 | G | C | 28.5% | 35.2 / 10.5 | 21 | intergenic (‑170/+70) | PP_3726/rocE | enoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE |
| Reads supporting (aligned to +/- strand): ref base G (10/5); new base C (3/3); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGGAGGACTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGG > NC_002947/4252104‑4252392 | gatgtaggcCTTGAGGTGGGAGATTCCAAGCGCGTGGTCAGCAACGCGGGCTTGGCTGTTACAACTTCGCTCAACTCGGTGTCTGGCGTTACACAAGTGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAggcgg < 2:143245/145‑1 (MQ=255) ttGGAGGACTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTAcgcggt > 2:176893/1‑149 (MQ=255) ggaggaCTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGgcgc < 2:257675/150‑1 (MQ=255) aggaCTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTAcgcggtgcgc > 1:606528/1‑145 (MQ=255) gaGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAg < 1:176893/150‑1 (MQ=37) gggAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGgc < 2:606528/150‑1 (MQ=37) gCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGCCCTCACAGGCGCTGAAAATGCAGCGACCAacac > 1:350971/1‑150 (MQ=255) gCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAacac > 2:735268/1‑150 (MQ=255) gCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAg > 2:355256/1‑150 (MQ=255) aaCTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGgtgc > 1:355034/1‑91 (MQ=25) aaCTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGgtgc < 2:355034/91‑1 (MQ=25) acaACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAAccc > 1:90168/1‑150 (MQ=255) tCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTg > 2:32176/1‑150 (MQ=255) cTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTgg < 1:735268/150‑1 (MQ=255) cGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGc < 2:90168/150‑1 (MQ=255) cacaAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAgg > 2:8849/1‑150 (MQ=255) aCGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCCAACCCCTCGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcggcggct > 2:129795/1‑148 (MQ=255) aCGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCCACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGtt < 2:350971/150‑1 (MQ=255) aCGGCTTTATGGCCTGTGAGATCGCGCACCGCGGAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAGCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGtt > 2:216809/1‑150 (MQ=255) tttATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACga > 2:47938/1‑150 (MQ=255) aTCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCAt > 1:229108/1‑150 (MQ=255) cgcgTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAggg > 2:233495/1‑150 (MQ=255) | GTTGGAGGACTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGG > NC_002947/4252104‑4252392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |