| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,330,680 | C→T | 11.1% | R28C (CGC→TGC) | PP_3802 → | cation ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,330,680 | 0 | C | T | 11.1% | 88.3 / 3.6 | 36 | R28C (CGC→TGC) | PP_3802 | cation ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (17/15); new base T (2/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
AGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGACCTTGCATTG > NC_002947/4330535‑4330819 | aGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCAt < 1:529891/150‑1 (MQ=255) ggAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAg > 1:212582/1‑150 (MQ=255) aaTGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTc > 2:2048/1‑150 (MQ=255) gcgcAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTc > 2:728892/1‑150 (MQ=255) cTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTgg > 1:506445/1‑150 (MQ=255) tCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATTGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGgc > 2:667218/1‑150 (MQ=255) cacGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGgcagca < 2:393324/150‑1 (MQ=255) ggAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCAtcgtc > 1:409287/1‑150 (MQ=255) aTGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTAGGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAAcggc > 1:354071/1‑150 (MQ=255) ccgccAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAaga < 2:217032/150‑1 (MQ=255) gccAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGTGCATcc > 2:687724/1‑76 (MQ=255) gccAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGTGCATcc > 2:687694/1‑76 (MQ=255) gccAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGTGCATcc < 1:687694/76‑1 (MQ=255) gccAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGTGCATcc < 1:687724/76‑1 (MQ=255) gCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGtt < 2:409287/150‑1 (MQ=255) gACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCaa < 1:176131/150‑1 (MQ=255) gACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCaa > 2:176131/1‑150 (MQ=255) gggCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGAGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTc > 1:644315/1‑150 (MQ=255) ggCCGCGCATCGATTTCACCGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCg < 2:494324/150‑1 (MQ=255) gcATCGATTTCACTGGCATTGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGa < 1:667218/150‑1 (MQ=255) gcATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGcagcag < 1:601837/78‑1 (MQ=255) gcATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGcagcag > 2:601837/1‑78 (MQ=255) gcATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGa < 1:2048/150‑1 (MQ=255) gATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGt < 1:423088/121‑1 (MQ=255) gATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGt > 2:423088/1‑121 (MQ=255) gATTTCACTGGCATCGACCCGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCca < 2:212582/150‑1 (MQ=255) tCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATTGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACAcc > 2:423493/1‑150 (MQ=255) aTCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAg > 1:533236/1‑150 (MQ=255) acctgacctTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGt > 1:724404/1‑64 (MQ=255) acctgacctTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGt < 2:724404/64‑1 (MQ=255) acctgacctTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGa > 2:305486/1‑150 (MQ=255) cctgacctTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTCCAGCTTGGCAGCAGGCAGCGTGCACGCCCTCGTCCGCCCCAACGGCGGCGGCCAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGAc > 2:222259/1‑150 (MQ=255) ctgacctTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGAcc > 2:473312/1‑150 (MQ=255) tGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATTGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGACCTTGCAtt < 1:423493/150‑1 (MQ=255) ggggCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGACCTTGCATTg < 2:506445/150‑1 (MQ=255) ggggCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGACCTTGCATTg < 1:728892/150‑1 (MQ=255) | AGTACGAGGTGGAAATGAAGCGCAACCTCGACACGGTGGTCCGCGCCATTCAGGAGAACCGCGCATGACCGCTGCCGCCAAGCTGGCGACAGCCTGCGGGCCGCGCATCGATTTCACTGGCATCGACCTGACCTTGGGGCGTACGCGCATCCTCGAACAGGTCAGTTTCAGCGTGGCAGCAGGCAGCGTGCACGCCATCGTCGGCCCCAACGGCGGCGGCAAGAGCTCGTTGATCAAGACCCTGCTCGGGCAGATGCCACACCAGGGCCAGCTGACCTTGCATTG > NC_002947/4330535‑4330819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |