| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,372,499 | A→G | 14.9% | intergenic (‑1040/‑532) | PP_5636 ← / → PP_3849 | hypothetical protein/hemolysin‑type calcium‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,372,499 | 0 | A | G | 14.9% | 53.2 / 4.2 | 27 | intergenic (‑1040/‑532) | PP_5636/PP_3849 | hypothetical protein/hemolysin‑type calcium‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (12/11); new base G (2/2); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01 | |||||||||||
TCGTAAATGGCAGGTGAGGTCTGATTACCTCAGAGAATTACCGTCCTGAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCT > NC_002947/4372351‑4372636 | tCGTAAATGGCAGGTGAGGTCTGATTACCTCAGAGAATTACCGTCCTGAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTAt > 1:686919/1‑150 (MQ=255)tCGTAAATGGCAGGTGAGGTCTGATTACCTCAGAGAATTACCGTCCTGAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTAt < 1:48596/150‑1 (MQ=255) cTCAGAGAATTACCGTCCTGAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCa > 1:597495/1‑150 (MQ=255) ttACCGTCCTGAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTAcc > 2:738754/1‑150 (MQ=255) tCCTGAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCAccc > 2:174401/1‑150 (MQ=255) gAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGa < 2:440100/120‑1 (MQ=255) gAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGa > 1:440100/1‑120 (MQ=255) ccATTATGTTTAAAATCGACATGAGCCCGAGACCTTCTGCGTCGCATATGTTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGtcatca < 2:686919/150‑1 (MQ=255) aaTCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCt < 2:597495/150‑1 (MQ=255) cccGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATc > 2:76875/1‑150 (MQ=255) cgcCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTc < 2:329591/150‑1 (MQ=255) ttCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTc < 1:516976/126‑1 (MQ=255) ttCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTc > 2:516976/1‑126 (MQ=255) acaAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGt > 1:378664/1‑150 (MQ=255) acaAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGt > 1:282474/1‑150 (MQ=255) aaaCTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTGTGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCt > 2:266621/1‑150 (MQ=255) aaaCTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTGTGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCt > 2:266693/1‑150 (MQ=255) tGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAAt < 2:282474/150‑1 (MQ=255) gCGAGCATCAGTCCAGCGTATACGCTCTAGATTTATGGATGGATAGATCATCATTGACCCACGCTCCAGACACTACCGCACCCAAACTGTCATACCTAACAATTCCTTCAATAGTAAATCTTTCGTCAGGCCGGTCTGGTCTTACAATAg > 1:581292/1‑150 (MQ=255) gCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACgg < 1:76875/150‑1 (MQ=255) taCGATCTAGATTAATTTGTGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGAt < 1:266693/150‑1 (MQ=255) taCGATCTAGATTAATTTGTGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGAt < 1:266621/150‑1 (MQ=255) taCGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGAt > 2:636307/1‑150 (MQ=255) cTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCt < 1:738754/150‑1 (MQ=255) cTAGATTAATTTATGGATAAATCATCAATGAGACACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCTGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCt < 1:175133/150‑1 (MQ=255) aTTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAgggcgggc > 2:435786/1‑106 (MQ=255) aTTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAgggcgggc < 1:435786/106‑1 (MQ=255) | TCGTAAATGGCAGGTGAGGTCTGATTACCTCAGAGAATTACCGTCCTGAACTGCTCCAGTATGTTGAAAATCGACATGAGCCCGCGCCATTCTGAGTAGCACAAACTTTGGCGAGCATCAGTCCAGCGTATACGATCTAGATTAATTTATGGATAAATCATCAATGAGCCACGCTCCAGACACTACCGCACCCAAACTGTCATCACTAACAATTCCCTCGATAGTAAATCTTTCGTCAGGGCGGGCTGGTCTTACAATAGACGGCCAAGCCACGGACGATTTATCT > NC_002947/4372351‑4372636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |