Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,449,607 A→G 8.3% intergenic (+78/‑364) nicR → / → nicA HTH‑type transcriptional repressor NicR/nicotinate dehydrogenase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,449,6070AG 8.3% 132.1 / 3.1 48intergenic (+78/‑364)nicR/nicAHTH‑type transcriptional repressor NicR/nicotinate dehydrogenase subunit A
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base G (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

AGCGCGTTGCCATTTTGTACCTGCTGCGCAAGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTCCTACACACA  >  NC_002947/4449468‑4449752
                                                                                                                                           |                                                                                                                                                 
aGCGCGTTGCCATTTTGTACCTGCTGCGCAAGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGacac                                                                                                                                         <  1:475223/150‑1 (MQ=255)
                    ctgctgCGCAAGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAg                                                                                                                     <  2:333788/150‑1 (MQ=255)
                    ctgctgCGCAAGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAg                                                                                                                     >  2:274220/1‑150 (MQ=255)
                             aaGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCggtgg                                                                                                                               >  1:171502/1‑131 (MQ=255)
                             aaGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCggtgg                                                                                                                               <  2:171502/131‑1 (MQ=255)
                              aGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAg                                                                                                           <  1:169745/150‑1 (MQ=255)
                              aGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAg                                                                                                           >  2:739736/1‑150 (MQ=255)
                                  gatCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGc                                                                                                       >  1:193185/1‑150 (MQ=255)
                                  gatCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCCGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAgtatc                                                                                                       >  2:53596/1‑146 (MQ=255)
                                    tCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGcag                                                                                                     <  1:676349/150‑3 (MQ=255)
                                    tCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCgg                                                                                                     >  2:97777/1‑150 (MQ=255)
                                    tCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCgg                                                                                                     <  1:625278/150‑1 (MQ=255)
                                    tCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCgg                                                                                                     <  1:622648/150‑1 (MQ=255)
                                     cGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTg                                                                                                                                              >  2:718080/1‑108 (MQ=255)
                                     cGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTg                                                                                                                                              <  1:718080/108‑1 (MQ=255)
                                      gACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGt                                                                                                   <  2:653470/150‑1 (MQ=255)
                                      gACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGt                                                                                                   <  1:97777/150‑1 (MQ=255)
                                      gACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGt                                                                                                   <  1:34050/150‑1 (MQ=255)
                                      gACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGt                                                                                                   >  2:63725/1‑150 (MQ=255)
                                        cAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTc                                                                                                 >  1:285558/1‑150 (MQ=255)
                                                 gaACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTAGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGaa                                                                                        <  1:53596/150‑1 (MQ=255)
                                                  aaCGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAAt                                                                                       >  1:643574/1‑150 (MQ=255)
                                                                aTTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCctgct                                                                         <  1:274220/150‑1 (MQ=255)
                                                                aTTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGAGTGCACCCGCGAATGGGCCGGCCctgct                                                                         >  2:658481/1‑150 (MQ=255)
                                                                 ttgttgTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCGCATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCcgc                                                                                           <  1:596852/131‑1 (MQ=255)
                                                                 ttgttgTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCGCATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCcgc                                                                                           >  2:596852/1‑131 (MQ=255)
                                                                 ttgttgTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCGCATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCcgc                                                                                           <  1:596687/131‑1 (MQ=255)
                                                                 ttgttgTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCGCATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCcgc                                                                                           >  2:596687/1‑131 (MQ=255)
                                                                        gACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGa                                                                                                         <  1:592601/110‑1 (MQ=255)
                                                                        gACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGa                                                                                                         >  2:592601/1‑110 (MQ=255)
                                                                          cTGCCGCCATTGTCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAAtctc                                                               >  2:91931/1‑150 (MQ=255)
                                                                                 cATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGc                                                        >  1:436167/1‑150 (MQ=255)
                                                                                  aTTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGc                                                        <  1:514339/149‑1 (MQ=255)
                                                                                  aTTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGc                                                        >  2:514339/1‑149 (MQ=255)
                                                                                         tCACAGATACGCGGCCTCAGCACGAAACTGTTTCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCAcc                                                <  2:548466/150‑1 (MQ=21)
                                                                                                            gagaTCAAGCGCATCGCGAGCAAGCTCGCTCACATAGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGc                                                                      <  2:353852/109‑1 (MQ=255)
                                                                                                            gagaTCAAGCGCATCGCGAGCAAGCTCGCTCACATAGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGc                                                                      >  1:353852/1‑109 (MQ=255)
                                                                                                                 cAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTcgcg                        >  2:216714/1‑150 (MQ=255)
                                                                                                                    gcgcATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGt                     >  1:212687/1‑150 (MQ=255)
                                                                                                                        aTCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAAc                 <  2:212687/150‑1 (MQ=255)
                                                                                                                              aGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTc           <  1:63725/150‑1 (MQ=255)
                                                                                                                                   gctcgctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTCCTaca      <  1:164611/150‑1 (MQ=255)
                                                                                                                                   gctcgctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTCCTaca      >  1:431047/1‑150 (MQ=255)
                                                                                                                                     tcgctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTCCTacaca    <  2:399921/150‑1 (MQ=255)
                                                                                                                                     tcgctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTCCTacaca    <  1:188792/150‑1 (MQ=255)
                                                                                                                                       gctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTcc          <  2:291072/142‑1 (MQ=255)
                                                                                                                                       gctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTCCTacacaca  <  2:643574/150‑1 (MQ=255)
                                                                                                                                       gctcACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACAACCGTATTCGCGGGTAAACCCGCTcc          >  1:291072/1‑142 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
AGCGCGTTGCCATTTTGTACCTGCTGCGCAAGATGATCGACAGCGACGAGAACGCGGGCTGAAGATTGTTGTGACTGCCGCCATTGCCGTCACAGATACGCGGCCTGAGAGATCAAGCGCATCGCGAGCAAGCTCGCTCACATTGGACACGCATCGGTGGTTGGACACAGGCCGCCGTAGGAGCGGGTTCACCCGCGAATGGGCCGGCCCTGCTGGCCAATCTCCACCTGCCGATCACCGTTGCCGCCACCACCGTATTCGCGGGTAAACCCGCTCCTACACACA  >  NC_002947/4449468‑4449752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑