| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,470,281 | G→A | 10.9% | T318I (ACC→ATC) | PP_3961 ← | transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,470,281 | 0 | G | A | 10.9% | 114.7 / 6.1 | 46 | T318I (ACC→ATC) | PP_3961 | transporter |
| Reads supporting (aligned to +/- strand): ref base G (19/22); new base A (2/3); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
CCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAGGA > NC_002947/4470141‑4470425 | cccACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCga > 2:569273/1‑150 (MQ=255) ccACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGCgat < 2:287414/150‑2 (MQ=255) ccACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCgaa < 2:48526/150‑1 (MQ=255) tCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAgg > 2:166060/1‑150 (MQ=255) ccTCCCCAGTTGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCaa < 1:407457/150‑1 (MQ=255) tgcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCCGGACATCCCTATGGACAGTTTGCGGTCTGGCGCGATGCGCTCTACCAGAGGCATGGCCACTAAGATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGAt < 1:390660/150‑1 (MQ=255) gcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATg < 1:497486/150‑1 (MQ=255) gcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATg < 1:45268/150‑1 (MQ=255) gcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATg < 1:45264/150‑1 (MQ=255) tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc < 1:207001/120‑1 (MQ=255) tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc > 2:207001/1‑120 (MQ=255) gaCCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCccaccacca < 2:295149/150‑1 (MQ=255) ccAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc > 1:407767/1‑96 (MQ=255) ccAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc < 2:407767/96‑1 (MQ=255) ccAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGGCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGt > 1:703976/1‑150 (MQ=255) ggTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCAATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGt > 1:271019/1‑150 (MQ=255) aTCCCTTCGGTAAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGCGGATTACTGCGATCAGCACGGCGATGGACAGCCCGATGATGTTTGTAACGAAGAGGAGCGGGAAGGTTGTcgcc > 1:317624/1‑150 (MQ=255) cTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCa < 1:489712/102‑1 (MQ=255) cTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCa > 2:489712/1‑102 (MQ=255) gcttTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAacccacc > 2:473690/1‑150 (MQ=255) cttTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAacccac > 1:400413/1‑148 (MQ=255) cttTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAacccac < 2:400413/148‑1 (MQ=255) tttCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc < 2:98875/70‑1 (MQ=255) tttCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc > 1:98875/1‑70 (MQ=255) ggCAAGATCCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGt < 1:596045/150‑1 (MQ=255) gCACGATGCGCTCTAACCGAAACATCGCCACACACTCTGTCGTGCCGGAGGATATTCCCGCAACCAGCACGGCGATGGCCAGCCCAATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTa < 2:271019/150‑1 (MQ=255) cGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTagca > 2:224187/1‑150 (MQ=255) gATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACcag > 2:399446/1‑102 (MQ=255) gATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACcag < 1:399446/102‑1 (MQ=255) gATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGGAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTagcag > 1:303380/1‑150 (MQ=255) gATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTagcag > 2:244690/1‑150 (MQ=255) aTGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTagcagc < 2:455419/150‑1 (MQ=255) gcgcTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCtt < 1:166060/150‑1 (MQ=255) gcgcTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCtt > 2:388036/1‑150 (MQ=255) ctctACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc < 1:749809/54‑1 (MQ=255) ctctACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAAc > 2:749809/1‑54 (MQ=255) ccAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCg > 1:86818/1‑150 (MQ=255) ccAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCg < 1:224187/150‑1 (MQ=255) cAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCgg < 1:569273/150‑1 (MQ=255) cttCGCCCCGACCATGATCGTGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATc > 1:319423/3‑150 (MQ=255) ttCGCCCCGACCATGATCGTGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg > 2:44180/2‑93 (MQ=38) ttCGCCCCGACCATGATCGTGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg < 1:44180/92‑1 (MQ=38) cgaccATGATCGTGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCa < 2:319423/146‑1 (MQ=255) gATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAg < 1:473690/150‑1 (MQ=255) gATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAg < 2:86818/150‑1 (MQ=255) tCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAGGa > 2:427256/1‑150 (MQ=255) | CCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAGGA > NC_002947/4470141‑4470425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |