| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,632,641 | G→C | 18.3% | noncoding (35/76 nt) | PP_t59 ← | tRNA‑Gly |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,632,641 | 0 | G | C | 18.3% | 63.1 / 6.0 | 27 | noncoding (35/76 nt) | PP_t59 | tRNA‑Gly |
| Reads supporting (aligned to +/- strand): ref base G (12/10); new base C (2/3); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAG > NC_002947/4632502‑4632786 | cGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggg < 1:434774/150‑3 (MQ=255) ggggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc > 1:393280/1‑150 (MQ=255) gTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctct < 2:393280/150‑1 (MQ=255) aGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAc > 1:42076/1‑150 (MQ=255) aaTGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCt > 2:99777/1‑150 (MQ=255) tggaacgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGGCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcg < 2:281731/148‑1 (MQ=255) cgaacgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCTGGAAACTAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgc < 2:273021/150‑1 (MQ=255) gaacgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgcg > 2:619929/1‑150 (MQ=255) cccGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCGTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGcc > 1:393064/1‑150 (MQ=255) cccGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGAACTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGcc > 1:478785/1‑150 (MQ=255) cGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCAt < 1:99777/150‑1 (MQ=255) cAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa < 1:287003/93‑1 (MQ=255) cAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa > 2:287003/1‑93 (MQ=255) ttCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAcccc > 2:303724/1‑150 (MQ=255) tCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCt > 2:68482/1‑150 (MQ=255) tGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGAt > 1:306034/1‑123 (MQ=255) tGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGAt < 2:306034/123‑1 (MQ=255) tGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCaaaaaa < 2:393064/150‑1 (MQ=255) ggaaaCTTGATCTCGTGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTcgc < 2:42076/146‑1 (MQ=255) tagagcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggc < 2:144022/140‑1 (MQ=255) tagagcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggc > 1:143988/8‑147 (MQ=255) tagagcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggc < 2:143988/140‑1 (MQ=255) tagagcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggc > 1:144022/8‑147 (MQ=255) cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcggc < 1:619929/150‑1 (MQ=255) gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcggccag > 2:563495/1‑150 (MQ=255) cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCTAGCCTTCGCGCAGTTCCGGCCTGGCGTCcagcag > 2:243554/1‑150 (MQ=39) cTTGGCAAGGTCGTGCTCTACCAACTCAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCcagcag < 1:303724/150‑1 (MQ=255) | CGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAG > NC_002947/4632502‑4632786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |