| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,645,521 | G→A | 7.2% | G14S (GGC→AGC) | fusB → | elongation factor G 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,645,521 | 0 | G | A | 7.2% | 150.6 / 2.8 | 56 | G14S (GGC→AGC) | fusB | elongation factor G 2 |
| Reads supporting (aligned to +/- strand): ref base G (28/23); new base A (2/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01 | |||||||||||
GGCCAGTGGATGCTGCAAGCCCAGACCGACTGACGGCCCGGTGGCAGGGCAGGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAACGCGGCATCACCAT > NC_002947/4645376‑4645669 | ggCCAGTGGATGCTGCAAGCCCAGACCGACTGACGGCCCGGTGGCAGGGCAGGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCAt > 1:511086/1‑150 (MQ=255) gATGCTGCAAGCCCAGACCGACTGACGGCCCGGTGGCAGGGCAGGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGccc > 1:683455/1‑150 (MQ=255) aTGCTGCAAGCCCAGACCGACTGACGGCCCGGTGGCAGGGCAGGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGGAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCa > 1:605853/1‑150 (MQ=255) aCCGACTGACGGCCCAGTGGCAGGGCAGGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCaa > 1:359795/1‑150 (MQ=255) gCCCGGTGGCAGGGCAGGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAgaccacgacca > 2:621076/1‑150 (MQ=255) ggcagggcagGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCg < 2:511086/150‑1 (MQ=255) gATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCAAGCTGTACCGCAATATCGGCATCGTCCCCCACGTGCATGCCTGCAAGACCACGACCCCCGAGAGGATccggcgc > 2:53589/1‑145 (MQ=255) tgTCTGCTAACTTGGTGGAATCCGTCCACCCATAGGTAGTCCGCCATGGCCCGCACAACGCCTATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCggg < 2:78680/149‑1 (MQ=255) tCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGt > 1:609168/1‑150 (MQ=255) tGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCa > 1:551461/1‑150 (MQ=255) gtggAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGTGGATCCTGTTCTACACCGGGGTCAACCACAAGAt < 2:483517/150‑1 (MQ=255) tggAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATg > 2:188789/1‑150 (MQ=255) gTCCACCCACAGGTAGTCCGCCATGGCCCGGACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGt > 2:9940/1‑150 (MQ=255) cacccacAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCa > 1:139460/1‑150 (MQ=255) acccacAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCAc > 1:207427/1‑150 (MQ=255) ccacAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGa < 2:271348/150‑1 (MQ=255) acaGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGGCGACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATg > 1:90085/1‑150 (MQ=255) aGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGc > 1:134873/1‑142 (MQ=255) aGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGc < 2:134873/142‑1 (MQ=255) gTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGa < 1:159407/144‑1 (MQ=255) gTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGa > 2:159407/1‑144 (MQ=255) gTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGgcgc > 2:695175/1‑150 (MQ=255) tCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCgcggcg > 2:337414/1‑150 (MQ=255) cGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGc > 1:579611/1‑54 (MQ=255) cGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGc < 2:579611/54‑1 (MQ=255) cGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGAc < 1:442403/150‑1 (MQ=255) ccATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATggg < 1:560373/124‑1 (MQ=255) ccATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATggg > 2:560373/1‑124 (MQ=255) tGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATgg < 1:621076/150‑1 (MQ=255) gCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGTAACGGCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGAc < 1:196672/150‑1 (MQ=255) gCCCGCACAACGCCCATCGAGCTGTACCGCAATATCAGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCggg < 2:472253/102‑1 (MQ=255) gCCCGCACAACGCCCATCGAGCTGTACCGCAATATCAGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCggg < 2:472242/102‑1 (MQ=255) gCCCGCACAACGCCCATCGAGCTGTACCGCAATATCAGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCggg > 1:472253/1‑102 (MQ=255) gCCCGCACAACGCCCATCGAGCTGTACCGCAATATCAGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCggg > 1:472242/1‑102 (MQ=255) acaACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATg > 2:390198/1‑150 (MQ=255) aCGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGcc < 1:9940/150‑1 (MQ=255) tctccATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGccc < 1:724931/147‑1 (MQ=255) gCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCa < 2:207427/150‑1 (MQ=255) ccATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAgg > 1:458981/1‑150 (MQ=255) cATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGtt > 2:187204/1‑78 (MQ=255) cATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGtt < 1:187204/78‑1 (MQ=255) cATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGa < 1:337414/150‑1 (MQ=255) cATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGAGCGAAGTGAACCCTGGCGCCGCGACCATGGACCGGATGGCCCaata > 2:724931/1‑147 (MQ=38) aTCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAg > 1:207328/1‑150 (MQ=255) cGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCa < 1:493832/150‑1 (MQ=255) gAGCTGCGCCACAATATCTGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAg < 2:605853/150‑1 (MQ=255) tGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAAc < 2:609168/150‑1 (MQ=255) ccGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAACGCgg < 1:695175/150‑1 (MQ=255) ccGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGGCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAACCCgg > 2:596126/1‑150 (MQ=255) cGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGTAGGAACGCGGc > 1:173787/1‑150 (MQ=255) cGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAACGCGGc < 2:139460/150‑1 (MQ=255) cGCAATATCGGCATCATCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCACGGACTGGATGGCCCAg > 1:378966/1‑135 (MQ=255) cGCAATATCGGCATCATCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCACGGACTGGATGGCCCAg < 2:378966/135‑1 (MQ=255) atCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAAcgc < 1:15971/141‑1 (MQ=255) atCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAAcgc > 2:15971/1‑141 (MQ=255) cGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAACGCGGcatcaccat < 2:683455/150‑1 (MQ=255) | GGCCAGTGGATGCTGCAAGCCCAGACCGACTGACGGCCCGGTGGCAGGGCAGGATTCCACCGGTCTGCTAACTTGGTGGAATCCGTCCACCCACAGGTAGTCCGCCATGGCCCGCACAACGCCCATCGAGCTGTACCGCAATATCGGCATCGTCGCCCACGTGGATGCCGGCAAGACCACGACCACCGAGCGGATCCTGTTCTACACCGGGGTCAACCACAAGATGGGCGAAGTGCACGATGGCGCGGCGACCATGGACTGGATGGCCCAGGAGCAGGAACGCGGCATCACCAT > NC_002947/4645376‑4645669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |