Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,666,126 G→A 12.5% A51T (GCC→ACC)  nuoK → NADH‑quinone oxidoreductase subunit K

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,666,1260GA12.5% 77.5 / 3.6 32A51T (GCC→ACC) nuoKNADH‑quinone oxidoreductase subunit K
Reads supporting (aligned to +/- strand):  ref base G (13/15);  new base A (2/2);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

TATCCCTCTCGAGCATGGGCTGGCGGTCGCCGGCATCCTGTTCTGCTTAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGC  >  NC_002947/4665984‑4666265
                                                                                                                                              |                                                                                                                                           
tATCCCTCTCGAGCATGGGCTGGCGGTCGCCGGCATCCTGTTCTGCTTAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGc                                                                                                                                      >  2:79200/1‑150 (MQ=255)
    ccTCTCGAGCATGGGCTGGCGGTCGCCGGCATCCTGTTCTGCTTAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCgg                                                                                                                                  <  2:651791/150‑2 (MQ=255)
                   cTGGCGGTCGCCGGCATCCTGTTCTGCGTAGGTGTGGTGGGCCTGGTGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGGCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTGGGGTCCAGCCCGAc                                                                                                                   <  2:137520/150‑1 (MQ=255)
                    tGGCGGTCGCCGGCATCCTGTTCTGCTTAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACg                                                                                                                  >  2:113225/1‑150 (MQ=255)
                                   tCCTGTTCTGCTTAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCa                                                                                                   <  1:681520/150‑1 (MQ=255)
                                        ttCTGCTTAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTg                                                                                              <  1:210453/150‑1 (MQ=255)
                                              ttAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTg                                                                                              <  2:732485/144‑1 (MQ=255)
                                              ttAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTg                                                                                              >  1:732485/1‑144 (MQ=255)
                                              ttAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATc                                                                                        <  1:113225/150‑1 (MQ=255)
                                                aGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTgg                                                                                                                               <  1:157189/109‑1 (MQ=255)
                                                aGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTgg                                                                                                                               >  2:157189/1‑109 (MQ=255)
                                                   tCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTgg                                                                                             <  1:228035/140‑1 (MQ=255)
                                                   tCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTgg                                                                                             >  2:228035/1‑140 (MQ=255)
                                                       gTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCa                                                                               >  1:573789/1‑150 (MQ=255)
                                                               gATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCAtt                                                                                                 >  1:254572/1‑124 (MQ=255)
                                                               gATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCAtt                                                                                                 <  2:254572/124‑1 (MQ=255)
                                                                   gTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGcc                                                                   >  2:743720/1‑150 (MQ=255)
                                                                    tCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCa                                                                  <  2:243746/150‑1 (MQ=255)
                                                                        ccgcAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCAt                                                              >  2:619875/1‑150 (MQ=255)
                                                                               aTCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATtggcctg                                                       <  1:164011/150‑1 (MQ=255)
                                                                               aTCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCACCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATtggcctg                                                       >  1:346716/1‑150 (MQ=255)
                                                                               aTCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCACCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATtggcctg                                                       >  1:346422/1‑150 (MQ=255)
                                                                                tCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATtggcctgg                                                      <  1:704715/150‑1 (MQ=255)
                                                                                         tGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCACCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCctgc                                             <  2:346422/150‑1 (MQ=255)
                                                                                         tGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCACCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCctgc                                             <  2:346716/150‑1 (MQ=255)
                                                                                                           tCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCt                           >  2:172646/1‑150 (MQ=255)
                                                                                                              tgatgaACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTg                                                                                  <  1:178721/92‑1 (MQ=255)
                                                                                                              tgatgaACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTg                                                                                  >  2:178721/1‑92 (MQ=255)
                                                                                                                  gaACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTCGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCacac                    >  1:715132/1‑150 (MQ=255)
                                                                                                                           tGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACAt           <  2:666085/150‑1 (MQ=255)
                                                                                                                              ccTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGa        <  1:172646/150‑1 (MQ=255)
                                                                                                                                    gTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGAtgctgc  >  1:490632/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TATCCCTCTCGAGCATGGGCTGGCGGTCGCCGGCATCCTGTTCTGCTTAGGTCTGGTTGGCCTGATGGTCCGCCGCAACATCCTCTTCGTGCTCATGAGCCTGGAAGTCATGATGAACGCCTCTGCCCTGGCGTTCGTCGTCGCCGGTGCCCGTTGGGTCCAGCCCGACGGTCAGGTGATGTTCATTCTGGTGATCAGCCTGGCAGCCGCCGAGGCCAGCATTGGCCTGGCCATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGC  >  NC_002947/4665984‑4666265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑