Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,765,978 C→G 9.8% P196A (CCG→GCG)  fpvA → TonB‑dependent outer membrane ferripyoverdine receptor FpvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,765,9780CG 9.8% 120.0 / 5.1 41P196A (CCG→GCG) fpvATonB‑dependent outer membrane ferripyoverdine receptor FpvA
Reads supporting (aligned to +/- strand):  ref base C (16/21);  new base G (2/2);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

AGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTG  >  NC_002947/4765832‑4766109
                                                                                                                                                  |                                                                                                                                   
aGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCgg                                                                                                                                  <  1:483743/150‑1 (MQ=255)
     gcgACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAAc                                                                                                                             >  1:669009/1‑150 (MQ=255)
       gACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAg                                                                                                                           >  1:644690/1‑150 (MQ=255)
       aaCCAAACTGCCACTGACCCTGCGCGAACCCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAg                                                                                                                           <  1:388694/149‑1 (MQ=255)
           aaaCTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAg                                                                                                                       >  1:72985/1‑150 (MQ=255)
              cTGCCACTGACCCTGCGCGACACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGCCCAAGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTAc                                                                                                                    <  1:546387/150‑1 (MQ=255)
               tGCCACTGACCCTGCGCGACACCCCGCGGGCCGTCACCGTGATCACCCGCCAGCGCATTGACGACCACCCGGTGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACt                                                                                                                   <  1:202891/150‑1 (MQ=255)
                             cgcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCttt                                                                                                     <  1:486188/150‑1 (MQ=255)
                             cgcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCttt                                                                                                     <  2:342901/150‑1 (MQ=255)
                              gcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTc                                                                                                    >  2:525425/1‑150 (MQ=255)
                                   aCCCCGCAGGCCGTCACCGTGATTACCCGCCAGCGCATTGTTGACCAGTCGATTCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCTCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGTCTTTCCCATc                                                                                               <  2:154477/150‑1 (MQ=255)
                                       cGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGGCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATcaaca                                                                                           >  2:54633/1‑150 (MQ=255)
                                               gTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATg                                                                                   >  1:57807/1‑150 (MQ=255)
                                                     gTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCCCTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt                                                                             <  2:12198/150‑1 (MQ=255)
                                                      tGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATg                                                                            >  2:679399/1‑150 (MQ=255)
                                                        aTCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGc                                                                          >  1:640575/1‑150 (MQ=255)
                                                          cACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCt                                                                        >  2:40646/1‑150 (MQ=255)
                                                             ccGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTTGGCCGGGCGCGCGGCCGACatgc                                                                                                                         <  2:382923/98‑4 (MQ=38)
                                                             ccGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTTGGCCGGGCGCGCGGCCGACatgc                                                                                                                         >  1:382923/1‑95 (MQ=38)
                                                                      cATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTa                                                            <  1:69484/150‑1 (MQ=255)
                                                                                cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGAc                                                  <  2:72985/150‑1 (MQ=255)
                                                                                 aGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACt                                                 <  1:145017/150‑1 (MQ=255)
                                                                                    cGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTggg                                              <  2:644690/150‑1 (MQ=255)
                                                                                    cGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCAGCGACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTggg                                              <  2:669009/150‑1 (MQ=255)
                                                                                             gCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTGCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCgg                                     <  2:277676/150‑1 (MQ=255)
                                                                                               cTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCaacaac                                                                                         <  2:174111/96‑1 (MQ=255)
                                                                                               cTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCaacaac                                                                                         >  1:174111/1‑96 (MQ=255)
                                                                                                        gTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATgg                            >  1:604190/1‑148 (MQ=255)
                                                                                                        gTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATgg                            <  2:604190/148‑1 (MQ=255)
                                                                                                        gTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGgcg                          >  1:625605/1‑150 (MQ=255)
                                                                                                         tGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGc                                                                      <  1:396651/105‑1 (MQ=255)
                                                                                                         tGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGc                                                                      >  2:396651/1‑105 (MQ=255)
                                                                                                          ggTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGcc                        <  1:40646/150‑1 (MQ=255)
                                                                                                            tCCATGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCCACAGCGGGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCt                      >  1:209012/1‑150 (MQ=255)
                                                                                                            tCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACt                                                 <  2:599627/123‑1 (MQ=255)
                                                                                                            tCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACt                                                 >  1:599627/1‑123 (MQ=255)
                                                                                                                    aCTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACcgc              <  2:57807/150‑1 (MQ=255)
                                                                                                                    aCTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGACAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACcgc              >  1:616427/1‑150 (MQ=255)
                                                                                                                                cgcATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTg  <  2:640575/150‑1 (MQ=255)
                                                                                                                                    tgttggCCGGGCGCGCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTgg                                               <  2:219683/97‑1 (MQ=255)
                                                                                                                                    tGTTGGCCGGGCGCGCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTgg                                               >  1:219683/1‑101 (MQ=255)
                                                                                                                                                  |                                                                                                                                   
AGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTG  >  NC_002947/4765832‑4766109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑