Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,868,154 T→G 9.3% C436G (TGC→GGC)  xdhA → xanthine dehydrogenase subunit XdhA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,868,1540TG 9.3% 105.2 / 4.7 43C436G (TGC→GGC) xdhAxanthine dehydrogenase subunit XdhA
Reads supporting (aligned to +/- strand):  ref base T (18/21);  new base G (2/2);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

CGCAGCCTTCAACCTGAGCATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCGGGTGACCGCTTATGTC  >  NC_002947/4868006‑4868300
                                                                                                                                                    |                                                                                                                                                  
cgcAGCCTTCAACCTGACCATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCACGCCCGCGCCTGTGAGGCGGCCCTGCGCGGCAGGGCGTGGCACCAGGCTGCCAGCCAGCGCGCCTg                                                                                                                                                   >  2:70140/1‑150 (MQ=255)
   aGCCTTCAACCTGAGCATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCa                                                                                                                                                >  2:618264/1‑150 (MQ=255)
   aGCCTTCAACCTGAGCATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCa                                                                                                                                                <  2:423311/150‑1 (MQ=255)
                  cATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGa                                                                                                                                 <  2:579319/150‑1 (MQ=255)
                      gAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATTGAGCGCGCCTGCCAGGCCCTGGCCGAg                                                                                                                                   <  1:396320/144‑1 (MQ=255)
                      gAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAg                                                                                                                                   >  2:396320/1‑144 (MQ=255)
                                   tCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCg                                                                                                                >  2:104704/1‑150 (MQ=255)
                                   tCAGAGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAgggc                                                                                                                                            <  2:222670/122‑3 (MQ=255)
                                   tCAGAGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAgggc                                                                                                                                            >  1:222670/1‑120 (MQ=255)
                                     aGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCCCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGAc                                                                                                              <  1:261966/150‑1 (MQ=255)
                                         gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 <  1:249953/127‑7 (MQ=255)
                                         gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 <  1:250144/127‑7 (MQ=255)
                                         gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 >  2:249953/1‑121 (MQ=255)
                                         gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 >  2:250144/1‑121 (MQ=255)
                                           gtgCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCcgc                                                                                                        <  1:104704/150‑1 (MQ=255)
                                              cGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGcc                                                                                                     <  1:618264/150‑1 (MQ=255)
                                                tATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAg                                                                                                   >  1:664750/1‑150 (MQ=255)
                                                 aTCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGc                                                                                                  >  1:418140/1‑150 (MQ=255)
                                                  tCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCa                                                                                                 >  2:106996/1‑150 (MQ=255)
                                                   cGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGCCTCCACCCCGCTCAGCGACTTCCGCGCCAGCaa                                                                                                <  1:700459/150‑1 (MQ=255)
                                                   cGCCTTCGGCGGCATGGCGGCCATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCCGCGACTTCCGCGCCAGCaa                                                                                                <  2:387005/150‑1 (MQ=255)
                                                           gcggcATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATAcc                                                                                        <  2:647638/150‑1 (MQ=255)
                                                             ggcATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCGAGCAAGGaagagctc                                                                                      <  2:749521/150‑7 (MQ=255)
                                                             ggcATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGc                                                                                      <  1:106996/150‑1 (MQ=255)
                                                                    cggcgATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGa                                                                               >  1:3996/1‑150 (MQ=255)
                                                                              cAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGaa                                                                     >  1:283663/1‑150 (MQ=255)
                                                                               aaGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAAc                                                                    >  2:156354/1‑150 (MQ=255)
                                                                                                 gAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTc                                                  <  2:3996/150‑1 (MQ=255)
                                                                                                   ggcggcGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCAt                                                <  1:327989/150‑1 (MQ=255)
                                                                                                       gcgcTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGaa                                            <  1:70140/150‑1 (MQ=255)
                                                                                                              gcgGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCaaa                                     <  1:82995/150‑1 (MQ=255)
                                                                                                                ggCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAaca                                   >  2:335511/1‑150 (MQ=255)
                                                                                                                 gCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAacac                                  >  2:418404/1‑150 (MQ=255)
                                                                                                                  cAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTCCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACAcc                                 <  2:701125/150‑1 (MQ=255)
                                                                                                                           ggACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACGTCGTACATCGa                        >  1:679298/1‑150 (MQ=255)
                                                                                                                             aCCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATTCCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGaaa                      >  1:577818/1‑150 (MQ=255)
                                                                                                                               cAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCCGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAAcc                    >  1:273506/1‑150 (MQ=255)
                                                                                                                                aGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAAccc                   <  2:283663/150‑1 (MQ=255)
                                                                                                                                 ggCTGCCATCGCCCGCGCCTTCTAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCg                  <  2:418140/150‑1 (MQ=255)
                                                                                                                                  gCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCgg                 <  2:577818/150‑1 (MQ=255)
                                                                                                                                      ccATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCgg                 <  2:579188/146‑1 (MQ=255)
                                                                                                                                      ccATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCgg                 >  1:579188/1‑146 (MQ=255)
                                                                                                                                                 gcCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCGGGTGACCGCTTATGTc  >  2:531358/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCAGCCTTCAACCTGAGCATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCGGGTGACCGCTTATGTC  >  NC_002947/4868006‑4868300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑