Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,868,161 C→G 10.0% A438G (GCC→GGC)  xdhA → xanthine dehydrogenase subunit XdhA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,868,1610CG10.0% 113.9 / 5.9 40A438G (GCC→GGC) xdhAxanthine dehydrogenase subunit XdhA
Reads supporting (aligned to +/- strand):  ref base C (16/20);  new base G (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

CATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCGGGTGACCGCTTATGTC  >  NC_002947/4868024‑4868300
                                                                                                                                         |                                                                                                                                           
cATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGa                                                                                                                                 <  2:579319/150‑1 (MQ=255)
    gAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATTGAGCGCGCCTGCCAGGCCCTGGCCGAg                                                                                                                                   <  1:396320/144‑1 (MQ=255)
    gAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAg                                                                                                                                   >  2:396320/1‑144 (MQ=255)
                 tCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCg                                                                                                                >  2:104704/1‑150 (MQ=255)
                   aGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCCCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGAc                                                                                                              <  1:261966/150‑1 (MQ=255)
                       gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 <  1:249953/127‑7 (MQ=255)
                       gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 <  1:250144/127‑7 (MQ=255)
                       gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 >  2:250144/1‑121 (MQ=255)
                       gtgtGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCGGCCAGGGCCTGGcaggcgc                                                                                                                                 >  2:249953/1‑121 (MQ=255)
                         gtgCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCcgc                                                                                                        <  1:104704/150‑1 (MQ=255)
                            cGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGcc                                                                                                     <  1:618264/150‑1 (MQ=255)
                              tATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAg                                                                                                   >  1:664750/1‑150 (MQ=255)
                               aTCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGc                                                                                                  >  1:418140/1‑150 (MQ=255)
                                tCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCa                                                                                                 >  2:106996/1‑150 (MQ=255)
                                 cGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGCCTCCACCCCGCTCAGCGACTTCCGCGCCAGCaa                                                                                                <  1:700459/150‑1 (MQ=255)
                                 cGCCTTCGGCGGCATGGCGGCCATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCCGCGACTTCCGCGCCAGCaa                                                                                                <  2:387005/150‑1 (MQ=255)
                                         gcggcATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATAcc                                                                                        <  2:647638/150‑1 (MQ=255)
                                           ggcATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCGAGCAAGGaagagctc                                                                                      <  2:749521/150‑7 (MQ=255)
                                           ggcATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGc                                                                                      <  1:106996/150‑1 (MQ=255)
                                                  cggcgATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGa                                                                               >  1:3996/1‑150 (MQ=255)
                                                            cAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGaa                                                                     >  1:283663/1‑150 (MQ=255)
                                                             aaGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAAc                                                                    >  2:156354/1‑150 (MQ=255)
                                                                               gAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTc                                                  <  2:3996/150‑1 (MQ=255)
                                                                                 ggcggcGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCAt                                                <  1:327989/150‑1 (MQ=255)
                                                                                     gcgcTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGaa                                            <  1:70140/150‑1 (MQ=255)
                                                                                            gcgGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCaaa                                     <  1:82995/150‑1 (MQ=255)
                                                                                              ggCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAaca                                   >  2:335511/1‑150 (MQ=255)
                                                                                               gCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAacac                                  >  2:418404/1‑150 (MQ=255)
                                                                                                cAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTCCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACAcc                                 <  2:701125/150‑1 (MQ=255)
                                                                                                         ggACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACGTCGTACATCGa                        >  1:679298/1‑150 (MQ=255)
                                                                                                           aCCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATTCCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGaaa                      >  1:577818/1‑150 (MQ=255)
                                                                                                             cAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCCGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAAcc                    >  1:273506/1‑150 (MQ=255)
                                                                                                              aGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAAccc                   <  2:283663/150‑1 (MQ=255)
                                                                                                               ggCTGCCATCGCCCGCGCCTTCTAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCg                  <  2:418140/150‑1 (MQ=255)
                                                                                                                gCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCgg                 <  2:577818/150‑1 (MQ=255)
                                                                                                                    ccATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCgg                 >  1:579188/1‑146 (MQ=255)
                                                                                                                    ccATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCgg                 <  2:579188/146‑1 (MQ=255)
                                                                                                                               gcCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCGGGTGACCGCTTATGTc  >  2:531358/1‑150 (MQ=255)
                                                                                                                                     cAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTgcgc                                                           >  1:579870/1‑87 (MQ=255)
                                                                                                                                     cAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTgcgc                                                           <  2:579870/87‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CATCGAAGACGGTGTGGTCAGCGGTGTGCGTATCGCCTTCGGCGGCATGGCGGCGATTCCCAAGCGCGCCCGCGCCTGTGAGGCGGCGCTGCGCGGCAAGCCGTGGGACCAGGCTGCCATCGAGCGCGCCTGCCAGGCCCTGGCCGAGGACTTCACCCCGCTCAGCGACTTCCGCGCCAGCAAGGAATACCGCCTGCTGACCGCGCAGAACCTGCTGCGCAAGTATTTCATCGAACAGCAAACACCGTACATCGAAACCCGGGTGACCGCTTATGTC  >  NC_002947/4868024‑4868300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑