| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,896,490 | G→A | 13.1% | intergenic (+78/‑130) | PP_4304 → / → sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,896,490 | 0 | G | A | 13.1% | 93.6 / 5.6 | 38 | intergenic (+78/‑130) | PP_4304/sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (18/15); new base A (2/3); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAACTCTTCACC > NC_002947/4896350‑4896637 | aCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgc > 1:273560‑M2/1‑141 (MQ=255) cGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaaga > 1:407579‑M2/1‑136 (MQ=255) aGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccg > 1:399228‑M2/1‑131 (MQ=255) ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCAt > 2:472710/1‑150 (MQ=255) tcGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGa < 1:165739/142‑1 (MQ=255) tcGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGa > 2:165739/1‑142 (MQ=255) gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCAtt > 1:135541/1‑150 (MQ=255) aaTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCAttt < 1:379374/150‑1 (MQ=255) gggTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCAtttttt < 2:428909/150‑1 (MQ=255) ttgttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGtt < 1:472710/150‑1 (MQ=255) tttCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaa < 2:594837‑M2/105‑13 (MQ=255) tttCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaa > 1:594837‑M2/1‑93 (MQ=255) ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTATGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtg < 2:157706‑M2/150‑92 (MQ=255) cgGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgcta < 2:686607‑M2/86‑32 (MQ=255) cgGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgcta > 1:686607‑M2/1‑55 (MQ=255) gggTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATc > 1:86397/1‑150 (MQ=255) gTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccg > 2:12771‑M2/1‑52 (MQ=255) gTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccg < 1:12771‑M2/71‑20 (MQ=255) aaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGccc < 2:86397/150‑1 (MQ=255) gCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgtggca < 2:381247‑M2/150‑107 (MQ=255) cTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGAtttacccgcgaagaggccggcgcaggcgctatc < 1:414450‑M2/76‑34 (MQ=255) cTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGAtttacccgcgaagaggccggcgcaggcgctatc > 2:414450‑M2/1‑43 (MQ=255) tCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggcctgcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtctt > 1:422582‑M2/1‑42 (MQ=255) tCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtctt < 2:422582‑M2/139‑98 (MQ=255) tCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGAtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgtggcagg < 2:711840‑M2/150‑109 (MQ=255) ccTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgag > 2:439516‑M2/1‑41 (MQ=255) ccTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgag < 1:439516‑M2/107‑67 (MQ=255) gcgcGGACCGTGCGCGGTCCCCCGTAGGAGCGGAtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgg < 1:674568‑M2/126‑93 (MQ=255) gcgcGGACCGTGCGCGGTCCCCCGTAGGAGCGGAtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgg > 2:674568‑M2/1‑34 (MQ=255) gcgGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgtggcaggtcgggcaatc < 2:455156‑M2/150‑119 (MQ=255) cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGtt > 2:263094/1‑94 (MQ=255) cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGtt < 1:263094/94‑1 (MQ=255) cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTGTTTGTTATTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGaaaa > 1:408920/1‑150 (MQ=255) gTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaa < 2:530938‑M2/84‑74 (MQ=255) gTAGGAGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaa > 1:530938‑M2/1‑11 (MQ=255) ttcgCGGGTAAACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAActct > 1:174464/4‑150 (MQ=255) ggAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAActct > 1:728613/1‑150 (MQ=255) aGCGGGtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgtggcaggtcgggcaatcatgctgcagctgctccccacgcatgg > 1:455156‑M2/1‑6 (MQ=255) gggTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAACTCTTCAcc < 2:135541/150‑1 (MQ=255) | ACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAACTCTTCACC > NC_002947/4896350‑4896637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |