| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,900,115 | T→C | 15.4% | intergenic (+31/‑467) | PP_4308 → / → PP_4309 | AsnC family transcriptional regulator/NCS1 family transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,900,115 | 0 | T | C | 15.4% | 50.2 / 3.8 | 26 | intergenic (+31/‑467) | PP_4308/PP_4309 | AsnC family transcriptional regulator/NCS1 family transporter |
| Reads supporting (aligned to +/- strand): ref base T (11/11); new base C (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01 | |||||||||||
TCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAACT > NC_002947/4899974‑4900262 | tCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGCCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGa < 1:646915/150‑1 (MQ=255) gAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGc < 2:391563/150‑1 (MQ=255) gcagcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAaga > 2:75212/1‑145 (MQ=255) gcagcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAaga < 1:75212/145‑1 (MQ=255) agcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGt > 1:81711/1‑150 (MQ=255) acCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGaaa > 2:96883/1‑150 (MQ=255) ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAg > 1:646982/1‑150 (MQ=255) aaTGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAACGGCGGTTGCCGCCAATGGATTGATTCGCTCCTGTGCTGGCCTCTTAGCAGGCGTGCGCCCGAAGAGGCCACGACACGAAAAGCTAGGTAATTTTTGtt > 2:281163/1‑149 (MQ=255) tctgagaCCAGTACCGCGATCATCAAGCAGACCCCGGTCAAGCGGCGGTGGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGCCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAAc < 1:717610/147‑1 (MQ=255) gCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAAc < 1:96883/150‑1 (MQ=255) gagaCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTa > 1:312324/1‑150 (MQ=255) aaTACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATa > 1:289660/1‑150 (MQ=255) aCCGTGATTATCAAGAAGCCCCCGGTCAAGCGGCCGTTGCCGCCAATGGATTGCTTTGCTCCTGTGCTGGCCTCTTCGCAGGGGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGt < 2:646982/150‑1 (MQ=255) caAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTtgt < 2:616495/150‑1 (MQ=255) ccGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTttc > 2:607571/1‑150 (MQ=255) cGGTGAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAGCGTAACCATAGGTGCCATTTTTGTGTGCACTttct < 1:281163/150‑1 (MQ=255) cAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAg < 2:312324/150‑1 (MQ=255) ttGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACACTTCTGACCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCa > 1:39160/1‑150 (MQ=255) gccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCg > 1:733351/1‑150 (MQ=255) cTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTAtttt < 2:289660/150‑1 (MQ=255) ctctTCGCGGGCACGCCTGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCa > 1:653721/1‑150 (MQ=255) tctTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCGGCATTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCaa > 1:609688/1‑150 (MQ=255) cAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCCTTACACACTTTCCACCCTCTATTTTGACCAAATGATc < 1:607571/150‑1 (MQ=255) gCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACtt > 2:385993/1‑120 (MQ=255) gCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACtt < 1:385993/120‑1 (MQ=255) cGTGCCCGCGACGAGGCCAGTACAAGCACAGCTGCGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAACt < 2:39160/150‑1 (MQ=255) | TCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAACT > NC_002947/4899974‑4900262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |