| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,909,967 | C→G | 14.0% | intergenic (+315/+164) | PP_4318 → / ← PP_4319 | transposase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,909,967 | 0 | C | G | 14.0% | 67.3 / 6.5 | 30 | intergenic (+315/+164) | PP_4318/PP_4319 | transposase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base G (2/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01 | |||||||||||
TTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAAGCAGAAGCAGCAGAAGC > NC_002947/4909820‑4910114 | tttCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCccatccat > 2:330254/1‑150 (MQ=37) gCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGc > 1:48168/1‑150 (MQ=32) gCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACACTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGc < 2:550279/150‑1 (MQ=17) cccTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGcc > 1:632468/1‑150 (MQ=32) gtcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCCGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAgcgc > 2:321570/3‑150 (MQ=17) gcgcGTCACGTTTTGTCAAACGCGAGAAAAAGTAACCAAAAAACACTGGCTCCTATCATCCGGCCTCTCCCCTACGCTCCGAGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGACCCATGTATGGGGCCtgtagcgc < 2:168497/150‑8 (MQ=11) cggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCtt < 1:479434/150‑1 (MQ=32) gccACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGTGGTTCCTTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGACCTCAGCGCTTGAc > 1:642801/3‑150 (MQ=255) ttGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATcc < 2:48168/150‑1 (MQ=32) cgaatgcgATAAAAAGTAATCAAAAAGCGCTGGCTTCGATTATCCGGCCCACGCGCCACGCTCCGGGGGTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGc < 2:552252/145‑1 (MQ=1) aaaCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGcc > 1:112502/1‑150 (MQ=32) aaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCt < 1:330254/150‑1 (MQ=32) ggCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCg < 1:62061/150‑1 (MQ=39) gCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCgg > 1:381321/1‑150 (MQ=39) tCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATACCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGcc > 2:730114/1‑150 (MQ=37) cGGGGTTCCCTCGCTACGGTCTTGTTCCCGGGAGTACGCGCTGCGGGCCCCATCCGTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGaa < 2:140098/150‑1 (MQ=255) gCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCt < 1:84266/150‑1 (MQ=255) cGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACa > 1:429320/1‑150 (MQ=255) gTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAg < 1:271924/150‑1 (MQ=255) cTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaag < 2:381321/150‑1 (MQ=255) cccGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGc > 2:20941/1‑119 (MQ=38) cccGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGc < 1:20941/119‑1 (MQ=38) gcTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCaaaa < 1:5738/134‑1 (MQ=255) gcTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCaaaa > 2:5738/1‑134 (MQ=255) ctgaGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGc > 2:399676/4‑99 (MQ=14) ctgaGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGc < 1:399676/96‑1 (MQ=14) aGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaagc < 1:581603/149‑1 (MQ=255) aGGCCCCATGGATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAaagcagaagcagaagcagaagc > 2:581603/1‑149 (MQ=255) ccccATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagca > 1:151804/1‑150 (MQ=255) cccATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCg < 2:403370/70‑1 (MQ=37) cccATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCg > 1:403370/1‑70 (MQ=37) ccatccatGGGGCCTCAGCGCTTGATGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagcaga > 2:490327/1‑150 (MQ=255) catccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagcagaa < 1:183900/150‑1 (MQ=255) catccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagcagaa < 2:642801/150‑1 (MQ=255) atccatGGGGCCTCAGCGCTTGACGGGCTTCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagcagaag > 2:28750/1‑150 (MQ=255) tccatGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAagcagaagcagcagaagc < 1:49628/150‑1 (MQ=255) | TTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGTCTTGCTCCCGGGAGTACGCGCTGCAGGCCCCATCCATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTTCTGAAGTCGCAATCTGCGTCGCCTGAACTACCGCGCGCTTACAAGCAAAAGCAGAAGCAGAAGCAGAAGCAGCAGAAGC > NC_002947/4909820‑4910114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |