Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,073,997 T→C 9.5% H58R (CAC→CGC)  PP_5691 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,073,9970TC 9.5% 113.2 / 2.7 42H58R (CAC→CGC) PP_5691hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/21);  new base C (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GCTCATCACTTCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCATGCCAGTGAGCACGGCCATCACCGAAAAGACCAGGGACGCCAA  >  NC_002947/5073857‑5074141
                                                                                                                                            |                                                                                                                                                
gCTCATCACTTCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGt                                                                                                                                         <  1:70692/150‑1 (MQ=255)
         ttCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCt                                                                                                                                           >  2:388278/1‑139 (MQ=255)
         ttCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCt                                                                                                                                           <  1:388278/139‑1 (MQ=255)
         ttCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCa                                                                                                                                >  2:326759/1‑150 (MQ=255)
         ttCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTCGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCa                                                                                                                                >  2:543200/1‑150 (MQ=255)
          tCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCaa                                                                                                                               <  2:76134/150‑1 (MQ=255)
           ccGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCCCGGTGTTAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAg                                                                                                                              <  1:654672/150‑1 (MQ=255)
                      ttGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGCACTTCGACGATGGCGAATTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGt                                                                                                                   <  2:273989/150‑1 (MQ=255)
                      ttGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGGCATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGt                                                                                                                   <  2:742202/150‑1 (MQ=255)
                          tGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACaa                                                                                                               >  2:438282/1‑150 (MQ=255)
                          cttACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACGCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGCGCTCGCTGTCTACCATCAAGGTGAACTCGGTACaa                                                                                                               <  1:15875/148‑1 (MQ=255)
                             ccTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGATTGCGCTAGCTGTCTACCATCAAGGTGAACTCGGTGCAAccg                                                                                                            >  2:15875/2‑148 (MQ=255)
                             aCTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGa                                                                                                            >  1:345440/1‑150 (MQ=255)
                             aCTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGCGCTCGCTGTCTACCATCAAGGTGAACTCGGTACaa                                                                                                               >  2:15703/1‑147 (MQ=255)
                             aCTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGCGCTCGCTGTCTACCATCAAGGTGAACTCGGTACaa                                                                                                               <  1:15703/147‑1 (MQ=255)
                                  ttCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCCTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTc                                                                                                       <  1:288344/150‑1 (MQ=255)
                                      aTTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGc                                                                                                   >  1:454539/1‑150 (MQ=255)
                                       ttCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCt                                                                                                  >  2:268748/1‑150 (MQ=255)
                                          tcCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGc                                                                                               >  2:636367/1‑150 (MQ=255)
                                             aCCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGc                                                                                            <  1:350276/150‑1 (MQ=255)
                                               ccATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGa                                                                                          >  1:713815/1‑150 (MQ=255)
                                                cATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGaa                                                                                         <  2:713815/150‑1 (MQ=255)
                                                       ctTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTATACCTTCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAg                                                                                  >  2:20060/1‑150 (MQ=255)
                                                         tGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGt                                                                                <  1:636367/150‑1 (MQ=255)
                                                                  aTGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAAt                                                                       <  2:118127/150‑1 (MQ=255)
                                                                  aTGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAAt                                                                       <  1:268748/150‑1 (MQ=255)
                                                                    gctggAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGc                                                                     <  2:345440/147‑1 (MQ=255)
                                                                    gCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGc                                                                     <  2:713276/150‑1 (MQ=255)
                                                                           tCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCa                                                              >  2:466120/1‑150 (MQ=255)
                                                                             aCGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTg                                                                                                                           >  2:97491/1‑87 (MQ=255)
                                                                             aCGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTg                                                                                                                           <  1:97491/87‑1 (MQ=255)
                                                                                             cgaTGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCAt                                            <  1:481786/150‑1 (MQ=255)
                                                                                                   cGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCCTCCTCAACATCCTAGTTACAGCATGCCAGt                                      <  1:20060/150‑1 (MQ=255)
                                                                                                     aaCTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCATGCCAGTGa                                    <  1:438282/150‑1 (MQ=255)
                                                                                                                      aaTGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTAGATCCCACAAGCGTCCTGCCGGCAGGGATTAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCATGCCAGTGAGCACGGCCATCACCGaa                   >  1:280196/1‑150 (MQ=255)
                                                                                                                        tGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACaa                                                                                                               <  2:454694/56‑1 (MQ=255)
                                                                                                                        tGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACaa                                                                                                               >  1:454694/1‑56 (MQ=255)
                                                                                                                            aCATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATcc                                                        <  1:643477/107‑1 (MQ=255)
                                                                                                                            aCATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATcc                                                        >  2:643477/1‑107 (MQ=255)
                                                                                                                             cATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCATGCCAGTGAGCACGGCCATCACCGAAAAGACCa            <  1:466120/150‑1 (MQ=255)
                                                                                                                                       gCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCATGCCAGTGAGCACGTCCATCACCGAAAAGACCAGGGACGCCaa  >  1:565746/1‑150 (MQ=255)
                                                                                                                                       gCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCATGCCAGTGAGCACGGCCATCACCGAAAAGACCAGGGACGCCaa  >  1:2265/1‑150 (MQ=255)
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GCTCATCACTTCCGATGATAACTTGATGTACTCGTTCCATTCTCCACCCATCGCTCTTGCTCATCCATGCGGGAATCACGGCGTGAACTTCGACGATGGCGAACTCGGCATCTTGGCTAATGAGACATTCTTGGAGCTTGTGCTCGCTGTCTACCATCAAGGTGAACTCGGTACAACGACCGTCCTGCTCGCTGCGAATAGACAGGTGATACCAATGCGTCCGCAACATCCTAGTTACAGCATGCCAGTGAGCACGGCCATCACCGAAAAGACCAGGGACGCCAA  >  NC_002947/5073857‑5074141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑